The RBPMSCreERT2-tdTomato mouse line for studying retinal and vascular relevant diseases
Summary: RNA-binding protein with multiple splicing (RBPMS) plays a crucial role in cardiac mesoderm specification and cardiovascular development, as well as being a typical marker for whole retinal ganglion cells (RGCs). However, there is a lack of animal models to spatiotemporally trace the locati...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223021880 |
| _version_ | 1797660134258769920 |
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| author | Guilan Li Yuanting Luo Qikai Zhang Wenfei Chen Kunbei Lai Yizhi Liu Yingfeng Zheng |
| author_facet | Guilan Li Yuanting Luo Qikai Zhang Wenfei Chen Kunbei Lai Yizhi Liu Yingfeng Zheng |
| author_sort | Guilan Li |
| collection | DOAJ |
| description | Summary: RNA-binding protein with multiple splicing (RBPMS) plays a crucial role in cardiac mesoderm specification and cardiovascular development, as well as being a typical marker for whole retinal ganglion cells (RGCs). However, there is a lack of animal models to spatiotemporally trace the location and function of RBPMS-expressing cells in vivo. In this study, we develop a tamoxifen-inducible RBPMS-tdTomato reporter mouse line to track RBPMS-expressing cells during embryogenesis and adulthood. This mouse line allows us to identify and locate RBPMS-tdTomato-positive cells among various tissues, especially in RGCs and smooth muscle cells, which assist to simulate related retinal degenerative diseases, model and examine choroidal neovascularization non-invasively in vivo. Our results show that the RBPMSCreERT2-tdTomato mouse line is a valuable tool for lineage tracing, disease modeling, drug screening, as well as isolating specific target cells. |
| first_indexed | 2024-03-11T18:25:22Z |
| format | Article |
| id | doaj.art-c165d89d517940499ce3e24d4497ad3e |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-11T18:25:22Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-c165d89d517940499ce3e24d4497ad3e2023-10-14T04:45:11ZengElsevieriScience2589-00422023-11-012611108111The RBPMSCreERT2-tdTomato mouse line for studying retinal and vascular relevant diseasesGuilan Li0Yuanting Luo1Qikai Zhang2Wenfei Chen3Kunbei Lai4Yizhi Liu5Yingfeng Zheng6State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou 510060, China; Research Units of Ocular Development and Regeneration, Chinese Academy of Medical Sciences, Beijing 100730, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou 510060, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou 510060, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou 510060, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou 510060, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou 510060, China; Research Units of Ocular Development and Regeneration, Chinese Academy of Medical Sciences, Beijing 100730, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou 510060, China; Research Units of Ocular Development and Regeneration, Chinese Academy of Medical Sciences, Beijing 100730, China; Corresponding authorSummary: RNA-binding protein with multiple splicing (RBPMS) plays a crucial role in cardiac mesoderm specification and cardiovascular development, as well as being a typical marker for whole retinal ganglion cells (RGCs). However, there is a lack of animal models to spatiotemporally trace the location and function of RBPMS-expressing cells in vivo. In this study, we develop a tamoxifen-inducible RBPMS-tdTomato reporter mouse line to track RBPMS-expressing cells during embryogenesis and adulthood. This mouse line allows us to identify and locate RBPMS-tdTomato-positive cells among various tissues, especially in RGCs and smooth muscle cells, which assist to simulate related retinal degenerative diseases, model and examine choroidal neovascularization non-invasively in vivo. Our results show that the RBPMSCreERT2-tdTomato mouse line is a valuable tool for lineage tracing, disease modeling, drug screening, as well as isolating specific target cells.http://www.sciencedirect.com/science/article/pii/S2589004223021880Natural sciencesBiological sciencesPhysiologyBiological sciences research methodologies |
| spellingShingle | Guilan Li Yuanting Luo Qikai Zhang Wenfei Chen Kunbei Lai Yizhi Liu Yingfeng Zheng The RBPMSCreERT2-tdTomato mouse line for studying retinal and vascular relevant diseases iScience Natural sciences Biological sciences Physiology Biological sciences research methodologies |
| title | The RBPMSCreERT2-tdTomato mouse line for studying retinal and vascular relevant diseases |
| title_full | The RBPMSCreERT2-tdTomato mouse line for studying retinal and vascular relevant diseases |
| title_fullStr | The RBPMSCreERT2-tdTomato mouse line for studying retinal and vascular relevant diseases |
| title_full_unstemmed | The RBPMSCreERT2-tdTomato mouse line for studying retinal and vascular relevant diseases |
| title_short | The RBPMSCreERT2-tdTomato mouse line for studying retinal and vascular relevant diseases |
| title_sort | rbpmscreert2 tdtomato mouse line for studying retinal and vascular relevant diseases |
| topic | Natural sciences Biological sciences Physiology Biological sciences research methodologies |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223021880 |
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