Pollen Viability of <i>Fraxinus excelsior</i> in Storage Experiments and Investigations on the Potential Effect of Long-Range Transport
Fragmented ash populations due to ash dieback may lead to a limited gene flow and pollination success. Therefore, the viability of ash pollen plays a major role for the survival of the species. The extent to which the long-distance transport of pollen affects pollen viability was investigated with e...
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MDPI AG
2022-04-01
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Online Access: | https://www.mdpi.com/1999-4907/13/4/600 |
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author | Lisa Buchner Anna-Katharina Eisen Branko Šikoparija Susanne Jochner-Oette |
author_facet | Lisa Buchner Anna-Katharina Eisen Branko Šikoparija Susanne Jochner-Oette |
author_sort | Lisa Buchner |
collection | DOAJ |
description | Fragmented ash populations due to ash dieback may lead to a limited gene flow and pollination success. Therefore, the viability of ash pollen plays a major role for the survival of the species. The extent to which the long-distance transport of pollen affects pollen viability was investigated with experiments in a climate chamber using ash pollen samples from a seed orchard in Emmendingen, Germany. Furthermore, experiments with a volumetric pollen trap were conducted. A suitable storage temperature for ash pollen was determined by using four viability tests; TTC test, pollen germination, Alexander’s stain and Acetocarmine. An optimization of the germination medium was performed. We found a strong influence of prevailing temperatures on pollen viability, which decreased faster under warmer conditions. At moderate temperatures, viable pollen could still be observed after 28 days. Thus, a possible successful pollination can also be associated to long-range transported pollen. Storage experiments showed that pollen viability could be maintained longer at temperatures of −20 °C and −80 °C than at 4 °C. In particular, the TTC test has proven to be suitable for determining viability. Therefore, properly stored pollen can be used for breeding programs to support the survival of <i>Fraxinus excelsior</i>. |
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language | English |
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spelling | doaj.art-c165f8b7e0f4465884587bc6d5f985502023-11-23T08:14:45ZengMDPI AGForests1999-49072022-04-0113460010.3390/f13040600Pollen Viability of <i>Fraxinus excelsior</i> in Storage Experiments and Investigations on the Potential Effect of Long-Range TransportLisa Buchner0Anna-Katharina Eisen1Branko Šikoparija2Susanne Jochner-Oette3Physical Geography/Landscape Ecology and Sustainable Ecosystem Development, Catholic University of Eichstätt-Ingolstadt, 85072 Eichstätt, GermanyPhysical Geography/Landscape Ecology and Sustainable Ecosystem Development, Catholic University of Eichstätt-Ingolstadt, 85072 Eichstätt, GermanyBioSense Institute—Research Institute for Information Technologies in Biosystems, University of Novi Sad, 21102 Novi Sad, SerbiaPhysical Geography/Landscape Ecology and Sustainable Ecosystem Development, Catholic University of Eichstätt-Ingolstadt, 85072 Eichstätt, GermanyFragmented ash populations due to ash dieback may lead to a limited gene flow and pollination success. Therefore, the viability of ash pollen plays a major role for the survival of the species. The extent to which the long-distance transport of pollen affects pollen viability was investigated with experiments in a climate chamber using ash pollen samples from a seed orchard in Emmendingen, Germany. Furthermore, experiments with a volumetric pollen trap were conducted. A suitable storage temperature for ash pollen was determined by using four viability tests; TTC test, pollen germination, Alexander’s stain and Acetocarmine. An optimization of the germination medium was performed. We found a strong influence of prevailing temperatures on pollen viability, which decreased faster under warmer conditions. At moderate temperatures, viable pollen could still be observed after 28 days. Thus, a possible successful pollination can also be associated to long-range transported pollen. Storage experiments showed that pollen viability could be maintained longer at temperatures of −20 °C and −80 °C than at 4 °C. In particular, the TTC test has proven to be suitable for determining viability. Therefore, properly stored pollen can be used for breeding programs to support the survival of <i>Fraxinus excelsior</i>.https://www.mdpi.com/1999-4907/13/4/600AcetocarmineAlexander’s stainash diebackpollen germinationTTCvolumetric pollen trap |
spellingShingle | Lisa Buchner Anna-Katharina Eisen Branko Šikoparija Susanne Jochner-Oette Pollen Viability of <i>Fraxinus excelsior</i> in Storage Experiments and Investigations on the Potential Effect of Long-Range Transport Forests Acetocarmine Alexander’s stain ash dieback pollen germination TTC volumetric pollen trap |
title | Pollen Viability of <i>Fraxinus excelsior</i> in Storage Experiments and Investigations on the Potential Effect of Long-Range Transport |
title_full | Pollen Viability of <i>Fraxinus excelsior</i> in Storage Experiments and Investigations on the Potential Effect of Long-Range Transport |
title_fullStr | Pollen Viability of <i>Fraxinus excelsior</i> in Storage Experiments and Investigations on the Potential Effect of Long-Range Transport |
title_full_unstemmed | Pollen Viability of <i>Fraxinus excelsior</i> in Storage Experiments and Investigations on the Potential Effect of Long-Range Transport |
title_short | Pollen Viability of <i>Fraxinus excelsior</i> in Storage Experiments and Investigations on the Potential Effect of Long-Range Transport |
title_sort | pollen viability of i fraxinus excelsior i in storage experiments and investigations on the potential effect of long range transport |
topic | Acetocarmine Alexander’s stain ash dieback pollen germination TTC volumetric pollen trap |
url | https://www.mdpi.com/1999-4907/13/4/600 |
work_keys_str_mv | AT lisabuchner pollenviabilityofifraxinusexcelsioriinstorageexperimentsandinvestigationsonthepotentialeffectoflongrangetransport AT annakatharinaeisen pollenviabilityofifraxinusexcelsioriinstorageexperimentsandinvestigationsonthepotentialeffectoflongrangetransport AT brankosikoparija pollenviabilityofifraxinusexcelsioriinstorageexperimentsandinvestigationsonthepotentialeffectoflongrangetransport AT susannejochneroette pollenviabilityofifraxinusexcelsioriinstorageexperimentsandinvestigationsonthepotentialeffectoflongrangetransport |