Molecular detection of feline hemoplasmas and retroviruses in free-roaming and shelter cats within a university campus
Objectives The aim of the present study was to assess the frequency of hemoplasma, feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) infections in cats living in an on-campus shelter and free-roaming cats within a university campus in Brazil. Methods Blood samples were tested usin...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2023-02-01
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| Series: | Journal of Feline Medicine and Surgery Open Reports |
| Online Access: | https://doi.org/10.1177/20551169221148672 |
| _version_ | 1811157710218985472 |
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| author | Ana Carolina Yamakawa Amanda Haisi Louise Bach Kmetiuk Maysa Pellizzaro Juliana Cristina Rebonato Mendes Aurea Maria Oliveira Canavessi Leila Sabrina Ullmann Wagner Antônio Chiba de Castro João Pessoa Araújo Júnior Andrea Pires dos Santos Alexander Welker Biondo |
| author_facet | Ana Carolina Yamakawa Amanda Haisi Louise Bach Kmetiuk Maysa Pellizzaro Juliana Cristina Rebonato Mendes Aurea Maria Oliveira Canavessi Leila Sabrina Ullmann Wagner Antônio Chiba de Castro João Pessoa Araújo Júnior Andrea Pires dos Santos Alexander Welker Biondo |
| author_sort | Ana Carolina Yamakawa |
| collection | DOAJ |
| description | Objectives The aim of the present study was to assess the frequency of hemoplasma, feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) infections in cats living in an on-campus shelter and free-roaming cats within a university campus in Brazil. Methods Blood samples were tested using quantitative PCR for hemoplasma, FIV and FeLV. Positive hemoplasma samples were sequenced. Associations between hemoplasma detection and living situation, sex, flea and/or tick parasitism, and coinfection with FIV and FeLV, were assessed using Fisher’s exact test and the respective odds ratios were calculated. Results Overall, 6/45 (13.3%) cats tested positive: four (8.9%) were infected with ‘ Candidatus Mycoplasma haemominutum’ and two (4.4%) with Mycoplasma haemofelis . All positive samples were from free-roaming cats (6/15; 40.0%) and had statistically significantly lower packed cell volumes ( P = 0.037). Although 5/23 (21.7%) males and 1/22 (4.6%) females were positive, no statistically significant association between sex and hemoplasma infection was found ( P = 0.19). Viral quantitative PCR (qPCR) was performed on 43/45 samples, among which 2/43 (4.7%) were positive for FIV and none for FeLV. Only one cat (2.3%) was coinfected with hemoplasma and FIV ( P = 0.26). In addition, 4/6 (66.7%) cats that tested positive for hemoplasmas were infested by fleas ( P = 0.0014) and/or ticks ( P = 0.25). Conclusions and relevance These results show that even if the free-roaming cat population is clinically healthy and has adequate access to food, it may present flea infestation and hemoplasma infection with lower packed cell volume values. |
| first_indexed | 2024-04-10T05:10:51Z |
| format | Article |
| id | doaj.art-c16bcc146b0b48888835878aaa86edc3 |
| institution | Directory Open Access Journal |
| issn | 2055-1169 |
| language | English |
| last_indexed | 2024-04-10T05:10:51Z |
| publishDate | 2023-02-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Feline Medicine and Surgery Open Reports |
| spelling | doaj.art-c16bcc146b0b48888835878aaa86edc32023-03-09T07:33:22ZengSAGE PublishingJournal of Feline Medicine and Surgery Open Reports2055-11692023-02-01910.1177/20551169221148672Molecular detection of feline hemoplasmas and retroviruses in free-roaming and shelter cats within a university campusAna Carolina Yamakawa0Amanda Haisi1Louise Bach Kmetiuk2Maysa Pellizzaro3Juliana Cristina Rebonato Mendes4Aurea Maria Oliveira Canavessi5Leila Sabrina Ullmann6Wagner Antônio Chiba de Castro7João Pessoa Araújo Júnior8Andrea Pires dos Santos9Alexander Welker Biondo10Biotechnology Institute, São Paulo State University (UNESP), Botucatu, SP, BrazilBiotechnology Institute, São Paulo State University (UNESP), Botucatu, SP, BrazilGraduate College of Cell and Molecular Biology, Federal University of Paraná (UFPR), R dos Funcionários, Curitiba, PR, BrazilInstitute of Collective Health, Federal University of Bahia, Salvador, BA, BrazilGraduate College of Cell and Molecular Biology, Federal University of Paraná (UFPR), R dos Funcionários, Curitiba, PR, BrazilDepartment of Animal Science, São Paulo University (USP), Piracicaba, SP, BrazilBiotechnology Institute, São Paulo State University (UNESP), Botucatu, SP, BrazilLatin-American Institute of Life and Nature Sciences, Federal University for Latin American Integration, Foz do Iguaçu, PR, BrazilBiotechnology Institute, São Paulo State University (UNESP), Botucatu, SP, BrazilDepartment of Comparative Pathobiology, Purdue University, West Lafayette, IN, USADepartment of Comparative Pathobiology, Purdue University, West Lafayette, IN, USAObjectives The aim of the present study was to assess the frequency of hemoplasma, feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) infections in cats living in an on-campus shelter and free-roaming cats within a university campus in Brazil. Methods Blood samples were tested using quantitative PCR for hemoplasma, FIV and FeLV. Positive hemoplasma samples were sequenced. Associations between hemoplasma detection and living situation, sex, flea and/or tick parasitism, and coinfection with FIV and FeLV, were assessed using Fisher’s exact test and the respective odds ratios were calculated. Results Overall, 6/45 (13.3%) cats tested positive: four (8.9%) were infected with ‘ Candidatus Mycoplasma haemominutum’ and two (4.4%) with Mycoplasma haemofelis . All positive samples were from free-roaming cats (6/15; 40.0%) and had statistically significantly lower packed cell volumes ( P = 0.037). Although 5/23 (21.7%) males and 1/22 (4.6%) females were positive, no statistically significant association between sex and hemoplasma infection was found ( P = 0.19). Viral quantitative PCR (qPCR) was performed on 43/45 samples, among which 2/43 (4.7%) were positive for FIV and none for FeLV. Only one cat (2.3%) was coinfected with hemoplasma and FIV ( P = 0.26). In addition, 4/6 (66.7%) cats that tested positive for hemoplasmas were infested by fleas ( P = 0.0014) and/or ticks ( P = 0.25). Conclusions and relevance These results show that even if the free-roaming cat population is clinically healthy and has adequate access to food, it may present flea infestation and hemoplasma infection with lower packed cell volume values.https://doi.org/10.1177/20551169221148672 |
| spellingShingle | Ana Carolina Yamakawa Amanda Haisi Louise Bach Kmetiuk Maysa Pellizzaro Juliana Cristina Rebonato Mendes Aurea Maria Oliveira Canavessi Leila Sabrina Ullmann Wagner Antônio Chiba de Castro João Pessoa Araújo Júnior Andrea Pires dos Santos Alexander Welker Biondo Molecular detection of feline hemoplasmas and retroviruses in free-roaming and shelter cats within a university campus Journal of Feline Medicine and Surgery Open Reports |
| title | Molecular detection of feline hemoplasmas and retroviruses in free-roaming and shelter cats within a university campus |
| title_full | Molecular detection of feline hemoplasmas and retroviruses in free-roaming and shelter cats within a university campus |
| title_fullStr | Molecular detection of feline hemoplasmas and retroviruses in free-roaming and shelter cats within a university campus |
| title_full_unstemmed | Molecular detection of feline hemoplasmas and retroviruses in free-roaming and shelter cats within a university campus |
| title_short | Molecular detection of feline hemoplasmas and retroviruses in free-roaming and shelter cats within a university campus |
| title_sort | molecular detection of feline hemoplasmas and retroviruses in free roaming and shelter cats within a university campus |
| url | https://doi.org/10.1177/20551169221148672 |
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