Dynamic force microscopy for imaging of viruses under physiological conditions
<p>Dynamic force microscopy (DFM) allows imaging of the structure and the assessment of the function of biological specimens in their physiological environment. In DFM, the cantilever is oscillated at a given frequency and touches the sample only at the end of its downward movement. Accordingl...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2004-01-01
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| Series: | Biological Procedures Online |
| Subjects: | |
| Online Access: | http://www.biologicalprocedures.com/bpo/arts/1/80/m80.htm |
| _version_ | 1811294571975409664 |
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| author | Kienberger Ferry Zhu Rong Moser Rosita Rankl Christian Blaas Dieter Hinterdorfer Peter |
| author_facet | Kienberger Ferry Zhu Rong Moser Rosita Rankl Christian Blaas Dieter Hinterdorfer Peter |
| author_sort | Kienberger Ferry |
| collection | DOAJ |
| description | <p>Dynamic force microscopy (DFM) allows imaging of the structure and the assessment of the function of biological specimens in their physiological environment. In DFM, the cantilever is oscillated at a given frequency and touches the sample only at the end of its downward movement. Accordingly, the problem of lateral forces displacing or even destroying bio-molecules is virtually inexistent as the contact time and friction forces are reduced. Here, we describe the use of DFM in studies of human rhinovirus serotype 2 (HRV2) weakly adhering to mica surfaces. The capsid of HRV2 was reproducibly imaged without any displacement of the virus. Release of the genomic RNA from the virions was initiated by exposure to low pH buffer and snapshots of the extrusion process were obtained. In the following, the technical details of previous DFM investigations of HRV2 are summarized. |
| first_indexed | 2024-04-13T05:19:32Z |
| format | Article |
| id | doaj.art-c16e4801ac164ed6993d1dcbf11cf474 |
| institution | Directory Open Access Journal |
| issn | 1480-9222 |
| language | English |
| last_indexed | 2024-04-13T05:19:32Z |
| publishDate | 2004-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Procedures Online |
| spelling | doaj.art-c16e4801ac164ed6993d1dcbf11cf4742022-12-22T03:00:47ZengBMCBiological Procedures Online1480-92222004-01-016112012810.1251/bpo80Dynamic force microscopy for imaging of viruses under physiological conditionsKienberger FerryZhu RongMoser RositaRankl ChristianBlaas DieterHinterdorfer Peter<p>Dynamic force microscopy (DFM) allows imaging of the structure and the assessment of the function of biological specimens in their physiological environment. In DFM, the cantilever is oscillated at a given frequency and touches the sample only at the end of its downward movement. Accordingly, the problem of lateral forces displacing or even destroying bio-molecules is virtually inexistent as the contact time and friction forces are reduced. Here, we describe the use of DFM in studies of human rhinovirus serotype 2 (HRV2) weakly adhering to mica surfaces. The capsid of HRV2 was reproducibly imaged without any displacement of the virus. Release of the genomic RNA from the virions was initiated by exposure to low pH buffer and snapshots of the extrusion process were obtained. In the following, the technical details of previous DFM investigations of HRV2 are summarized.http://www.biologicalprocedures.com/bpo/arts/1/80/m80.htmMicroscopy, Atomic ForceRhinovirus |
| spellingShingle | Kienberger Ferry Zhu Rong Moser Rosita Rankl Christian Blaas Dieter Hinterdorfer Peter Dynamic force microscopy for imaging of viruses under physiological conditions Biological Procedures Online Microscopy, Atomic Force Rhinovirus |
| title | Dynamic force microscopy for imaging of viruses under physiological conditions |
| title_full | Dynamic force microscopy for imaging of viruses under physiological conditions |
| title_fullStr | Dynamic force microscopy for imaging of viruses under physiological conditions |
| title_full_unstemmed | Dynamic force microscopy for imaging of viruses under physiological conditions |
| title_short | Dynamic force microscopy for imaging of viruses under physiological conditions |
| title_sort | dynamic force microscopy for imaging of viruses under physiological conditions |
| topic | Microscopy, Atomic Force Rhinovirus |
| url | http://www.biologicalprocedures.com/bpo/arts/1/80/m80.htm |
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