Quantitation of glutathione S-transferases in rice (Oryza sativa L.) roots exposed to cadmium by liquid chromatography–tandem mass spectrometry using isotope-labeled wing peptides as an internal standard

Quantitation of glutathione S-transferases in rice (Oryza sativa L.) roots exposed to cadmium by liquid chromatography–tandem mass spectrometry using isotope-labeled wing peptides as an internal standard

Abstract Background Plant glutathione S-transferases (GSTs, EC 2.5.1.18) are multifunctional enzymes involved in heavy metal cellular detoxification by conjugating the tripeptide (g-Glu-Cys-Gly) glutathione to heavy metals. Previous studies demonstrated that individual rice GSTs were differentially...

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Bibliographic Details
Main Authors: Zhenzhen Cao, Renxiang Mou, Zhaoyun Cao, Xiaoyan Lin, Youning Ma, Zhiwei Zhu, Mingxue Chen
Format: Article
Language:English
Published: BMC 2017-08-01
Series:Plant Methods
Subjects:
Glutathione S-transferases
Cadmium
Liquid chromatography–tandem mass spectrometry
Signature peptide
Isotope-labeled internal standard
Online Access:http://link.springer.com/article/10.1186/s13007-017-0214-2

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