miR-26b enhances radiosensitivity of hepatocellular carcinoma cells by targeting EphA2
Objective(s): Although low-dose radiotherapy (RT) that involves low collateral damage is more suitable for hepatocellular carcinoma (HCC) than traditional high-dose RT, but to achieve satisfactory therapeutic effect with low-dose RT, it is necessary to sensitize HCC cells to irradiation. This study...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2016-08-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_7466_cb9fbd875247deab0dd04ecd495054ab.pdf |
| _version_ | 1818777149619830784 |
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| author | Qiao Jin Xiang Jun Li Pei Guo Cao |
| author_facet | Qiao Jin Xiang Jun Li Pei Guo Cao |
| author_sort | Qiao Jin |
| collection | DOAJ |
| description | Objective(s): Although low-dose radiotherapy (RT) that involves low collateral damage is more suitable for hepatocellular carcinoma (HCC) than traditional high-dose RT, but to achieve satisfactory therapeutic effect with low-dose RT, it is necessary to sensitize HCC cells to irradiation. This study was aimed to determine whether radiosensitivity of HCC cells can be enhanced using miR-26b by targeting erythropoietin producing human hepatocelluar A2 (EphA2).
Materials and Methods: The levels of miR-26b and EphA2 expression in multiple HCC cell lines were assessed by qPCR and western blotting, respectively, and compared with those in a hepatic cell line. HCC 97H cells were transfected with miR-26b mimics, EphA2-ShRNA or EphA2 over-expression vector before exposure to low-dose irradiation.
Results:Different degrees of miR-26b down-regulation and EphA2 up-regulation were observed in all HCC cell lines, among which the HCC 97H cell line expressed the lowest level of miR-26b and highest level of EphA2. EphA2 was verified as the target of miR-26b by dual luciferase reporter assay. HCC 97H cells transfected with miR-26b mimics or EphA2-ShRNA reduced the expression of EphA2 protein, with significantly lower cell proliferation rate and cell invasion ability and higher apoptosis rate in response to low-dose irradiation than those in the non-transfected cells. These results were reversed after EphA2 was overexpressed by transfection with the EphA2 overexpression vector. Co-transfection with miR-26b mimics and EphA2 overexpression vector barely altered EphA2 expression level and cell response to low-dose irradiation.
Conclusion: These data suggest that miR-26b enhances radiosensitivity of HCC 97H cells by targeting EphA2 protein. |
| first_indexed | 2024-12-18T11:24:14Z |
| format | Article |
| id | doaj.art-c177508209a4419f96ed88b64072e02c |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-18T11:24:14Z |
| publishDate | 2016-08-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-c177508209a4419f96ed88b64072e02c2022-12-21T21:09:46ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742016-08-0119885185710.22038/ijbms.2016.74667466miR-26b enhances radiosensitivity of hepatocellular carcinoma cells by targeting EphA2Qiao Jin0Xiang Jun Li1Pei Guo Cao2Department of Oncology, Third Xiangya Hospital, Central South University, NO.138, Tong Zi Po Road, Yue Lu District, Chang Sha, Hunan Province, China 410013Department of Oncology, Third Xiangya Hospital, Central South University, NO.138, Tong Zi Po Road, Yue Lu District, Chang Sha, Hunan Province, China 410013Department of Oncology, Third Xiangya Hospital, Central South University, NO.138, Tong Zi Po Road, Yue Lu District, Chang Sha, Hunan Province, China 410013Objective(s): Although low-dose radiotherapy (RT) that involves low collateral damage is more suitable for hepatocellular carcinoma (HCC) than traditional high-dose RT, but to achieve satisfactory therapeutic effect with low-dose RT, it is necessary to sensitize HCC cells to irradiation. This study was aimed to determine whether radiosensitivity of HCC cells can be enhanced using miR-26b by targeting erythropoietin producing human hepatocelluar A2 (EphA2). Materials and Methods: The levels of miR-26b and EphA2 expression in multiple HCC cell lines were assessed by qPCR and western blotting, respectively, and compared with those in a hepatic cell line. HCC 97H cells were transfected with miR-26b mimics, EphA2-ShRNA or EphA2 over-expression vector before exposure to low-dose irradiation. Results:Different degrees of miR-26b down-regulation and EphA2 up-regulation were observed in all HCC cell lines, among which the HCC 97H cell line expressed the lowest level of miR-26b and highest level of EphA2. EphA2 was verified as the target of miR-26b by dual luciferase reporter assay. HCC 97H cells transfected with miR-26b mimics or EphA2-ShRNA reduced the expression of EphA2 protein, with significantly lower cell proliferation rate and cell invasion ability and higher apoptosis rate in response to low-dose irradiation than those in the non-transfected cells. These results were reversed after EphA2 was overexpressed by transfection with the EphA2 overexpression vector. Co-transfection with miR-26b mimics and EphA2 overexpression vector barely altered EphA2 expression level and cell response to low-dose irradiation. Conclusion: These data suggest that miR-26b enhances radiosensitivity of HCC 97H cells by targeting EphA2 protein.http://ijbms.mums.ac.ir/article_7466_cb9fbd875247deab0dd04ecd495054ab.pdfEphA2HCC 97H cellsHepatocellular carcinomamiR-26Radiosensitivity |
| spellingShingle | Qiao Jin Xiang Jun Li Pei Guo Cao miR-26b enhances radiosensitivity of hepatocellular carcinoma cells by targeting EphA2 Iranian Journal of Basic Medical Sciences EphA2 HCC 97H cells Hepatocellular carcinoma miR-26 Radiosensitivity |
| title | miR-26b enhances radiosensitivity of hepatocellular carcinoma cells by targeting EphA2 |
| title_full | miR-26b enhances radiosensitivity of hepatocellular carcinoma cells by targeting EphA2 |
| title_fullStr | miR-26b enhances radiosensitivity of hepatocellular carcinoma cells by targeting EphA2 |
| title_full_unstemmed | miR-26b enhances radiosensitivity of hepatocellular carcinoma cells by targeting EphA2 |
| title_short | miR-26b enhances radiosensitivity of hepatocellular carcinoma cells by targeting EphA2 |
| title_sort | mir 26b enhances radiosensitivity of hepatocellular carcinoma cells by targeting epha2 |
| topic | EphA2 HCC 97H cells Hepatocellular carcinoma miR-26 Radiosensitivity |
| url | http://ijbms.mums.ac.ir/article_7466_cb9fbd875247deab0dd04ecd495054ab.pdf |
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