Laminin Levels Regulate Tissue Migration and Anterior-Posterior Polarity during Egg Morphogenesis in Drosophila
Basement membranes (BMs) are specialized extracellular matrices required for tissue organization and organ formation. We study the role of laminin and its integrin receptor in the regulation of tissue migration during Drosophila oogenesis. Egg production in Drosophila involves the collective migrati...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-07-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124717308288 |
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| author | María C. Díaz de la Loza Alfonsa Díaz-Torres Federico Zurita Alicia E. Rosales-Nieves Emad Moeendarbary Kristian Franze María D. Martín-Bermudo Acaimo González-Reyes |
| author_facet | María C. Díaz de la Loza Alfonsa Díaz-Torres Federico Zurita Alicia E. Rosales-Nieves Emad Moeendarbary Kristian Franze María D. Martín-Bermudo Acaimo González-Reyes |
| author_sort | María C. Díaz de la Loza |
| collection | DOAJ |
| description | Basement membranes (BMs) are specialized extracellular matrices required for tissue organization and organ formation. We study the role of laminin and its integrin receptor in the regulation of tissue migration during Drosophila oogenesis. Egg production in Drosophila involves the collective migration of follicle cells (FCs) over the BM to shape the mature egg. We show that laminin content in the BM increases with time, whereas integrin amounts in FCs do not vary significantly. Manipulation of integrin and laminin levels reveals that a dynamic balance of integrin-laminin amounts determines the onset and speed of FC migration. Thus, the interplay of ligand-receptor levels regulates tissue migration in vivo. Laminin depletion also affects the ultrastructure and biophysical properties of the BM and results in anterior-posterior misorientation of developing follicles. Laminin emerges as a key player in the regulation of collective cell migration, tissue stiffness, and the organization of anterior-posterior polarity in Drosophila. |
| first_indexed | 2024-12-23T05:25:09Z |
| format | Article |
| id | doaj.art-c177ef9acf044e52823a0b9e5be7451a |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-23T05:25:09Z |
| publishDate | 2017-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-c177ef9acf044e52823a0b9e5be7451a2022-12-21T17:58:37ZengElsevierCell Reports2211-12472017-07-0120121122310.1016/j.celrep.2017.06.031Laminin Levels Regulate Tissue Migration and Anterior-Posterior Polarity during Egg Morphogenesis in DrosophilaMaría C. Díaz de la Loza0Alfonsa Díaz-Torres1Federico Zurita2Alicia E. Rosales-Nieves3Emad Moeendarbary4Kristian Franze5María D. Martín-Bermudo6Acaimo González-Reyes7Centro Andaluz de Biología del Desarrollo, CSIC/Universidad Pablo de Olavide/JA, Carretera de Utrera km 1, 41013 Sevilla, SpainCentro Andaluz de Biología del Desarrollo, CSIC/Universidad Pablo de Olavide/JA, Carretera de Utrera km 1, 41013 Sevilla, SpainDepartamento de Genética e Instituto de Biotecnología, Universidad de Granada, Centro de Investigación Biomédica, 18071 Granada, SpainCentro Andaluz de Biología del Desarrollo, CSIC/Universidad Pablo de Olavide/JA, Carretera de Utrera km 1, 41013 Sevilla, SpainDepartment of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3DY, UKDepartment of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3DY, UKCentro Andaluz de Biología del Desarrollo, CSIC/Universidad Pablo de Olavide/JA, Carretera de Utrera km 1, 41013 Sevilla, SpainCentro Andaluz de Biología del Desarrollo, CSIC/Universidad Pablo de Olavide/JA, Carretera de Utrera km 1, 41013 Sevilla, SpainBasement membranes (BMs) are specialized extracellular matrices required for tissue organization and organ formation. We study the role of laminin and its integrin receptor in the regulation of tissue migration during Drosophila oogenesis. Egg production in Drosophila involves the collective migration of follicle cells (FCs) over the BM to shape the mature egg. We show that laminin content in the BM increases with time, whereas integrin amounts in FCs do not vary significantly. Manipulation of integrin and laminin levels reveals that a dynamic balance of integrin-laminin amounts determines the onset and speed of FC migration. Thus, the interplay of ligand-receptor levels regulates tissue migration in vivo. Laminin depletion also affects the ultrastructure and biophysical properties of the BM and results in anterior-posterior misorientation of developing follicles. Laminin emerges as a key player in the regulation of collective cell migration, tissue stiffness, and the organization of anterior-posterior polarity in Drosophila.http://www.sciencedirect.com/science/article/pii/S2211124717308288collective migrationepithelial migrationlamininintegrinanterior-posterior polarityDrosophila oogenesis |
| spellingShingle | María C. Díaz de la Loza Alfonsa Díaz-Torres Federico Zurita Alicia E. Rosales-Nieves Emad Moeendarbary Kristian Franze María D. Martín-Bermudo Acaimo González-Reyes Laminin Levels Regulate Tissue Migration and Anterior-Posterior Polarity during Egg Morphogenesis in Drosophila Cell Reports collective migration epithelial migration laminin integrin anterior-posterior polarity Drosophila oogenesis |
| title | Laminin Levels Regulate Tissue Migration and Anterior-Posterior Polarity during Egg Morphogenesis in Drosophila |
| title_full | Laminin Levels Regulate Tissue Migration and Anterior-Posterior Polarity during Egg Morphogenesis in Drosophila |
| title_fullStr | Laminin Levels Regulate Tissue Migration and Anterior-Posterior Polarity during Egg Morphogenesis in Drosophila |
| title_full_unstemmed | Laminin Levels Regulate Tissue Migration and Anterior-Posterior Polarity during Egg Morphogenesis in Drosophila |
| title_short | Laminin Levels Regulate Tissue Migration and Anterior-Posterior Polarity during Egg Morphogenesis in Drosophila |
| title_sort | laminin levels regulate tissue migration and anterior posterior polarity during egg morphogenesis in drosophila |
| topic | collective migration epithelial migration laminin integrin anterior-posterior polarity Drosophila oogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S2211124717308288 |
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