From Normal to Obesity and Back: The Associations between Mitochondrial DNA Copy Number, Gender, and Body Mass Index
Mitochondrial DNA (mtDNA) encodes core subunits of oxidative phosphorylation complexes and, as a result of intricate regulatory crosstalk between nuclear and mitochondrial genomes, the total number of mtDNA copies fits the requirements of each cell type. Deviations from the physiological number of m...
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MDPI AG
2019-05-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/8/5/430 |
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| author | Daria Skuratovskaia Larisa Litvinova Maria Vulf Pavel Zatolokin Konstantin Popadin Ilia Mazunin |
| author_facet | Daria Skuratovskaia Larisa Litvinova Maria Vulf Pavel Zatolokin Konstantin Popadin Ilia Mazunin |
| author_sort | Daria Skuratovskaia |
| collection | DOAJ |
| description | Mitochondrial DNA (mtDNA) encodes core subunits of oxidative phosphorylation complexes and, as a result of intricate regulatory crosstalk between nuclear and mitochondrial genomes, the total number of mtDNA copies fits the requirements of each cell type. Deviations from the physiological number of mtDNA copies are expected to be deleterious and might cause some inherited diseases and normal ageing. We studied 46 obese patients with type 2 diabetes (T2DM) one year after a laparoscopic sleeve gastrectomy (LSG) and Roux-en-Y gastric bypass (RYGB). The results were compared with normal-weight patients without T2DM (control group 1) (body mass index (BMI) = 22.5 ± 3.01 kg/m<sup>2</sup>) and patients with obesity without T2DM (control group 2) (BMI = 36 ± 3.45 kg/m<sup>2</sup>). We detected an increase of mtDNA copy number in the cells of the buffy coat obtained from peripheral blood, sampled one year after bariatric surgery. We also found that average mtDNA copy number as well as its dynamics (before and after the surgery) are gender-specific. To the best of our knowledge, this is the first evidence for the restoration of mtDNA copy number in obese patients after LSG and RYGB. |
| first_indexed | 2024-03-12T06:40:28Z |
| format | Article |
| id | doaj.art-c179079ce8eb4f70ac13a8ac8c9a4e9e |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T06:40:28Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-c179079ce8eb4f70ac13a8ac8c9a4e9e2023-09-03T01:03:11ZengMDPI AGCells2073-44092019-05-018543010.3390/cells8050430cells8050430From Normal to Obesity and Back: The Associations between Mitochondrial DNA Copy Number, Gender, and Body Mass IndexDaria Skuratovskaia0Larisa Litvinova1Maria Vulf2Pavel Zatolokin3Konstantin Popadin4Ilia Mazunin5Laboratory of Immunology and Cell Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236016, RussiaLaboratory of Immunology and Cell Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236016, RussiaLaboratory of Immunology and Cell Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236016, RussiaLaboratory of Immunology and Cell Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236016, RussiaCenter for Mitochondrial Functional Genomics, Institute of Living Systems, Immanuel Kant Baltic Federal University, Kaliningrad 236040, RussiaCenter for Mitochondrial Functional Genomics, Institute of Living Systems, Immanuel Kant Baltic Federal University, Kaliningrad 236040, RussiaMitochondrial DNA (mtDNA) encodes core subunits of oxidative phosphorylation complexes and, as a result of intricate regulatory crosstalk between nuclear and mitochondrial genomes, the total number of mtDNA copies fits the requirements of each cell type. Deviations from the physiological number of mtDNA copies are expected to be deleterious and might cause some inherited diseases and normal ageing. We studied 46 obese patients with type 2 diabetes (T2DM) one year after a laparoscopic sleeve gastrectomy (LSG) and Roux-en-Y gastric bypass (RYGB). The results were compared with normal-weight patients without T2DM (control group 1) (body mass index (BMI) = 22.5 ± 3.01 kg/m<sup>2</sup>) and patients with obesity without T2DM (control group 2) (BMI = 36 ± 3.45 kg/m<sup>2</sup>). We detected an increase of mtDNA copy number in the cells of the buffy coat obtained from peripheral blood, sampled one year after bariatric surgery. We also found that average mtDNA copy number as well as its dynamics (before and after the surgery) are gender-specific. To the best of our knowledge, this is the first evidence for the restoration of mtDNA copy number in obese patients after LSG and RYGB.https://www.mdpi.com/2073-4409/8/5/430mtDNA copiesbariatric surgerytype 2 diabetesobesityddPCR |
| spellingShingle | Daria Skuratovskaia Larisa Litvinova Maria Vulf Pavel Zatolokin Konstantin Popadin Ilia Mazunin From Normal to Obesity and Back: The Associations between Mitochondrial DNA Copy Number, Gender, and Body Mass Index Cells mtDNA copies bariatric surgery type 2 diabetes obesity ddPCR |
| title | From Normal to Obesity and Back: The Associations between Mitochondrial DNA Copy Number, Gender, and Body Mass Index |
| title_full | From Normal to Obesity and Back: The Associations between Mitochondrial DNA Copy Number, Gender, and Body Mass Index |
| title_fullStr | From Normal to Obesity and Back: The Associations between Mitochondrial DNA Copy Number, Gender, and Body Mass Index |
| title_full_unstemmed | From Normal to Obesity and Back: The Associations between Mitochondrial DNA Copy Number, Gender, and Body Mass Index |
| title_short | From Normal to Obesity and Back: The Associations between Mitochondrial DNA Copy Number, Gender, and Body Mass Index |
| title_sort | from normal to obesity and back the associations between mitochondrial dna copy number gender and body mass index |
| topic | mtDNA copies bariatric surgery type 2 diabetes obesity ddPCR |
| url | https://www.mdpi.com/2073-4409/8/5/430 |
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