Bioactivity-Guided Isolation of Ethyl-<em>p</em>-methoxycinnamate, an Anti-inflammatory Constituent, from <em>Kaempferia galanga</em> L. Extracts
This study evaluated the anti-inflammatory effect of <em>Kaempferia galanga </em>(KG) using an activity-guided approach. KG rhizomes were serially extracted with petroleum ether, chloroform, methanol and water. These extracts (2 g/kg each) were tested for their ability to...
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MDPI AG
2012-07-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/17/7/8720 |
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| author | Ashfaq Ahmed Rabia Altaf Mun Fei Yam Item J. Atangwho Mohd Zaini Asmawi Amirin Sadikun Muhammad Ihtisham Umar |
| author_facet | Ashfaq Ahmed Rabia Altaf Mun Fei Yam Item J. Atangwho Mohd Zaini Asmawi Amirin Sadikun Muhammad Ihtisham Umar |
| author_sort | Ashfaq Ahmed |
| collection | DOAJ |
| description | This study evaluated the anti-inflammatory effect of <em>Kaempferia galanga </em>(KG) using an activity-guided approach. KG rhizomes were serially extracted with petroleum ether, chloroform, methanol and water. These extracts (2 g/kg each) were tested for their ability to inhibit carrageenan-induced rat paw edema. The chloroform extract was found to exert the highest inhibition (42.9%) compared to control (<em>p</em> < 0.001), hence it was further fractionated by washing serially with hexane, hexane-chloroform (1:1) and chloroform. The chloroform fraction (1 g/kg) showed the highest inhibitory effect (51.9%, <em>p</em> < 0.001) on carrageenan-induced edema. This chloroform fraction was further fractionated with hexane-chloroform (1:3) and chloroform, and of the two fractions, the hexane-chloroform sub-fraction was the most effective in inhibiting edema (53.7%, <em>p</em> < 0.001). GC-MS analysis of the active sub-fraction identified ethyl-<em>p</em>-methoxycinnamate (EPMC) as the major component, which was re-crystallized. EPMC dose-dependently inhibited carrageenan-induced edema with an MIC of 100 mg/kg. Moreover, in an <em>in vitro</em> study, EPMC non-selectively inhibited the activities of cyclooxygenases 1 and 2, with IC<sub>50</sub> values of 1.12 µM and 0.83 µM respectively. These results validate the anti-inflammatory activity of KG which may be exerted by the inhibition of cyclooxygenases 1 and 2. EPMC isolated from this plant may be the active anti-inflammatory agent. |
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| language | English |
| last_indexed | 2024-12-22T02:19:07Z |
| publishDate | 2012-07-01 |
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| series | Molecules |
| spelling | doaj.art-c184ac56ef594978a5c52c47d5f951ec2022-12-21T18:42:12ZengMDPI AGMolecules1420-30492012-07-011778720873410.3390/molecules17078720Bioactivity-Guided Isolation of Ethyl-<em>p</em>-methoxycinnamate, an Anti-inflammatory Constituent, from <em>Kaempferia galanga</em> L. ExtractsAshfaq AhmedRabia AltafMun Fei YamItem J. AtangwhoMohd Zaini AsmawiAmirin SadikunMuhammad Ihtisham UmarThis study evaluated the anti-inflammatory effect of <em>Kaempferia galanga </em>(KG) using an activity-guided approach. KG rhizomes were serially extracted with petroleum ether, chloroform, methanol and water. These extracts (2 g/kg each) were tested for their ability to inhibit carrageenan-induced rat paw edema. The chloroform extract was found to exert the highest inhibition (42.9%) compared to control (<em>p</em> < 0.001), hence it was further fractionated by washing serially with hexane, hexane-chloroform (1:1) and chloroform. The chloroform fraction (1 g/kg) showed the highest inhibitory effect (51.9%, <em>p</em> < 0.001) on carrageenan-induced edema. This chloroform fraction was further fractionated with hexane-chloroform (1:3) and chloroform, and of the two fractions, the hexane-chloroform sub-fraction was the most effective in inhibiting edema (53.7%, <em>p</em> < 0.001). GC-MS analysis of the active sub-fraction identified ethyl-<em>p</em>-methoxycinnamate (EPMC) as the major component, which was re-crystallized. EPMC dose-dependently inhibited carrageenan-induced edema with an MIC of 100 mg/kg. Moreover, in an <em>in vitro</em> study, EPMC non-selectively inhibited the activities of cyclooxygenases 1 and 2, with IC<sub>50</sub> values of 1.12 µM and 0.83 µM respectively. These results validate the anti-inflammatory activity of KG which may be exerted by the inhibition of cyclooxygenases 1 and 2. EPMC isolated from this plant may be the active anti-inflammatory agent.http://www.mdpi.com/1420-3049/17/7/8720ethyl-<em>p</em>-methoxycinnamatecarrageenan-induced edemacyclooxygenase<em>Kaempferia galanga</em><em> </em>anti-inflammatory activity |
| spellingShingle | Ashfaq Ahmed Rabia Altaf Mun Fei Yam Item J. Atangwho Mohd Zaini Asmawi Amirin Sadikun Muhammad Ihtisham Umar Bioactivity-Guided Isolation of Ethyl-<em>p</em>-methoxycinnamate, an Anti-inflammatory Constituent, from <em>Kaempferia galanga</em> L. Extracts Molecules ethyl-<em>p</em>-methoxycinnamate carrageenan-induced edema cyclooxygenase <em>Kaempferia galanga</em> <em> </em>anti-inflammatory activity |
| title | Bioactivity-Guided Isolation of Ethyl-<em>p</em>-methoxycinnamate, an Anti-inflammatory Constituent, from <em>Kaempferia galanga</em> L. Extracts |
| title_full | Bioactivity-Guided Isolation of Ethyl-<em>p</em>-methoxycinnamate, an Anti-inflammatory Constituent, from <em>Kaempferia galanga</em> L. Extracts |
| title_fullStr | Bioactivity-Guided Isolation of Ethyl-<em>p</em>-methoxycinnamate, an Anti-inflammatory Constituent, from <em>Kaempferia galanga</em> L. Extracts |
| title_full_unstemmed | Bioactivity-Guided Isolation of Ethyl-<em>p</em>-methoxycinnamate, an Anti-inflammatory Constituent, from <em>Kaempferia galanga</em> L. Extracts |
| title_short | Bioactivity-Guided Isolation of Ethyl-<em>p</em>-methoxycinnamate, an Anti-inflammatory Constituent, from <em>Kaempferia galanga</em> L. Extracts |
| title_sort | bioactivity guided isolation of ethyl lt em gt p lt em gt methoxycinnamate an anti inflammatory constituent from lt em gt kaempferia galanga lt em gt l extracts |
| topic | ethyl-<em>p</em>-methoxycinnamate carrageenan-induced edema cyclooxygenase <em>Kaempferia galanga</em> <em> </em>anti-inflammatory activity |
| url | http://www.mdpi.com/1420-3049/17/7/8720 |
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