Evaluation of the Saponin Content in <i>Panax vietnamensis</i> Acclimatized to Lam Dong Province by HPLC–UV/CAD

<i>Panax vietnamensis</i>, or Vietnamese ginseng (VG), an endemic <i>Panax</i> species in Vietnam, possesses a unique saponin profile and interesting biological activities. This plant is presently in danger of extinction due to over-exploitation, resulting in many preservatio...

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Main Authors: Huy Truong Nguyen, Kim Long Vu-Huynh, Hien Minh Nguyen, Huong Thuy Le, Thi Hong Van Le, Jeong Hill Park, Minh Duc Nguyen
Format: Article
Language:English
Published: MDPI AG 2021-09-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/26/17/5373
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author Huy Truong Nguyen
Kim Long Vu-Huynh
Hien Minh Nguyen
Huong Thuy Le
Thi Hong Van Le
Jeong Hill Park
Minh Duc Nguyen
author_facet Huy Truong Nguyen
Kim Long Vu-Huynh
Hien Minh Nguyen
Huong Thuy Le
Thi Hong Van Le
Jeong Hill Park
Minh Duc Nguyen
author_sort Huy Truong Nguyen
collection DOAJ
description <i>Panax vietnamensis</i>, or Vietnamese ginseng (VG), an endemic <i>Panax</i> species in Vietnam, possesses a unique saponin profile and interesting biological activities. This plant is presently in danger of extinction due to over-exploitation, resulting in many preservation efforts towards the geographical acclimatization of VG. Yet, no information on the saponin content of the acclimatized VG, an important quality indicator, is available. Here, we analyzed the saponin content in the underground parts of two- to five-year-old VG plants acclimatized to Lam Dong province. Nine characteristic saponins, including notoginsenoside-R1, ginsenoside-Rg1, -Rb1, -Rd, majonoside-R1, -R2 vina-ginsenoside-R2, -R11, and pseudoginsenoside-RT4, were simultaneously determined by HPLC coupled with UV and with a charged aerosol detector (CAD). Analyzing the results illustrated that the detection of characteristic ocotillol-type saponins in VG by CAD presented a superior capacity compared with that of UV, thus implying a preferential choice of CAD for the analysis of VG. The quantitative results indicating the saponin content in the underground parts of VG showed an increasing tendency from two to five years old, with the root and the rhizome exhibiting different saponin accumulation patterns. This is the first study that reveals the preliminary success of VG acclimatization and thereby encourages the continuing efforts to develop this valuable saponin-rich plant.
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spelling doaj.art-c18a7271653042738ecacb5c04e127222023-11-22T11:03:09ZengMDPI AGMolecules1420-30492021-09-012617537310.3390/molecules26175373Evaluation of the Saponin Content in <i>Panax vietnamensis</i> Acclimatized to Lam Dong Province by HPLC–UV/CADHuy Truong Nguyen0Kim Long Vu-Huynh1Hien Minh Nguyen2Huong Thuy Le3Thi Hong Van Le4Jeong Hill Park5Minh Duc Nguyen6Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, VietnamFaculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, VietnamFaculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, VietnamFaculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, VietnamFaculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City 700000, VietnamCollege of Pharmacy, Seoul National University, Seoul 08826, KoreaFaculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, Vietnam<i>Panax vietnamensis</i>, or Vietnamese ginseng (VG), an endemic <i>Panax</i> species in Vietnam, possesses a unique saponin profile and interesting biological activities. This plant is presently in danger of extinction due to over-exploitation, resulting in many preservation efforts towards the geographical acclimatization of VG. Yet, no information on the saponin content of the acclimatized VG, an important quality indicator, is available. Here, we analyzed the saponin content in the underground parts of two- to five-year-old VG plants acclimatized to Lam Dong province. Nine characteristic saponins, including notoginsenoside-R1, ginsenoside-Rg1, -Rb1, -Rd, majonoside-R1, -R2 vina-ginsenoside-R2, -R11, and pseudoginsenoside-RT4, were simultaneously determined by HPLC coupled with UV and with a charged aerosol detector (CAD). Analyzing the results illustrated that the detection of characteristic ocotillol-type saponins in VG by CAD presented a superior capacity compared with that of UV, thus implying a preferential choice of CAD for the analysis of VG. The quantitative results indicating the saponin content in the underground parts of VG showed an increasing tendency from two to five years old, with the root and the rhizome exhibiting different saponin accumulation patterns. This is the first study that reveals the preliminary success of VG acclimatization and thereby encourages the continuing efforts to develop this valuable saponin-rich plant.https://www.mdpi.com/1420-3049/26/17/5373<i>Panax vietnamensis</i>Vietnamese ginsengHPLC–CADmajonosidevina-ginsenosideocotillol
spellingShingle Huy Truong Nguyen
Kim Long Vu-Huynh
Hien Minh Nguyen
Huong Thuy Le
Thi Hong Van Le
Jeong Hill Park
Minh Duc Nguyen
Evaluation of the Saponin Content in <i>Panax vietnamensis</i> Acclimatized to Lam Dong Province by HPLC–UV/CAD
Molecules
<i>Panax vietnamensis</i>
Vietnamese ginseng
HPLC–CAD
majonoside
vina-ginsenoside
ocotillol
title Evaluation of the Saponin Content in <i>Panax vietnamensis</i> Acclimatized to Lam Dong Province by HPLC–UV/CAD
title_full Evaluation of the Saponin Content in <i>Panax vietnamensis</i> Acclimatized to Lam Dong Province by HPLC–UV/CAD
title_fullStr Evaluation of the Saponin Content in <i>Panax vietnamensis</i> Acclimatized to Lam Dong Province by HPLC–UV/CAD
title_full_unstemmed Evaluation of the Saponin Content in <i>Panax vietnamensis</i> Acclimatized to Lam Dong Province by HPLC–UV/CAD
title_short Evaluation of the Saponin Content in <i>Panax vietnamensis</i> Acclimatized to Lam Dong Province by HPLC–UV/CAD
title_sort evaluation of the saponin content in i panax vietnamensis i acclimatized to lam dong province by hplc uv cad
topic <i>Panax vietnamensis</i>
Vietnamese ginseng
HPLC–CAD
majonoside
vina-ginsenoside
ocotillol
url https://www.mdpi.com/1420-3049/26/17/5373
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