Alterations of Oxidative Phosphorylation Complexes in Papillary Thyroid Carcinoma
The papillary thyroid carcinoma (PTC) is the most common malignant tumor of the thyroid gland, with disruptive mutations in mitochondrial complex I subunits reported at very low frequency. Furthermore, metabolic diversity of PTC has been postulated owing to variable messenger RNA (mRNA) expression o...
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MDPI AG
2018-05-01
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| Series: | Cells |
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| Online Access: | http://www.mdpi.com/2073-4409/7/5/40 |
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| author | Franz A. Zimmermann Daniel Neureiter Wolfgang Sperl Johannes A. Mayr Barbara Kofler |
| author_facet | Franz A. Zimmermann Daniel Neureiter Wolfgang Sperl Johannes A. Mayr Barbara Kofler |
| author_sort | Franz A. Zimmermann |
| collection | DOAJ |
| description | The papillary thyroid carcinoma (PTC) is the most common malignant tumor of the thyroid gland, with disruptive mutations in mitochondrial complex I subunits reported at very low frequency. Furthermore, metabolic diversity of PTC has been postulated owing to variable messenger RNA (mRNA) expression of genes encoding subunits of the oxidative phosphorylation (OXHPOS) complexes. The aim of the present study was to evaluate the metabolic diversity of the OXPHOS system at the protein level by using immunohistochemical staining. Analysis of 18 human PTCs revealed elevated mitochondrial biogenesis but significantly lower levels of OXPHOS complex I in the tumor tissue (p < 0.0001) compared to the adjacent normal tissue. In contrast, OXPHOS complexes II–V were increased in the majority of PTCs. In three PTCs, we found pathologic mutations within mitochondrially encoded complex I subunits. Our data indicate that PTCs are characterized by an oncocytic metabolic signature that is in low complex I is combined with elevated mitochondrial mass and high complex II–V levels, which might be an important factor for tumor formation. |
| first_indexed | 2024-03-12T20:04:06Z |
| format | Article |
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| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T20:04:06Z |
| publishDate | 2018-05-01 |
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| spelling | doaj.art-c18a86e3f44f493c83bbea0490add8802023-08-02T02:14:53ZengMDPI AGCells2073-44092018-05-01754010.3390/cells7050040cells7050040Alterations of Oxidative Phosphorylation Complexes in Papillary Thyroid CarcinomaFranz A. Zimmermann0Daniel Neureiter1Wolfgang Sperl2Johannes A. Mayr3Barbara Kofler4Research Program for Receptor Biochemistry and Tumor Metabolism, University Hospital Salzburg, Paracelsus Medical University, Salzburg 5020, AustriaDepartment of Pathology, University Hospital Salzburg, Paracelsus Medical University, Salzburg 5020, AustriaDepartment of Pediatrics, University Hospital Salzburg, Paracelsus Medical University, Salzburg 5020, AustriaDepartment of Pediatrics, University Hospital Salzburg, Paracelsus Medical University, Salzburg 5020, AustriaResearch Program for Receptor Biochemistry and Tumor Metabolism, University Hospital Salzburg, Paracelsus Medical University, Salzburg 5020, AustriaThe papillary thyroid carcinoma (PTC) is the most common malignant tumor of the thyroid gland, with disruptive mutations in mitochondrial complex I subunits reported at very low frequency. Furthermore, metabolic diversity of PTC has been postulated owing to variable messenger RNA (mRNA) expression of genes encoding subunits of the oxidative phosphorylation (OXHPOS) complexes. The aim of the present study was to evaluate the metabolic diversity of the OXPHOS system at the protein level by using immunohistochemical staining. Analysis of 18 human PTCs revealed elevated mitochondrial biogenesis but significantly lower levels of OXPHOS complex I in the tumor tissue (p < 0.0001) compared to the adjacent normal tissue. In contrast, OXPHOS complexes II–V were increased in the majority of PTCs. In three PTCs, we found pathologic mutations within mitochondrially encoded complex I subunits. Our data indicate that PTCs are characterized by an oncocytic metabolic signature that is in low complex I is combined with elevated mitochondrial mass and high complex II–V levels, which might be an important factor for tumor formation.http://www.mdpi.com/2073-4409/7/5/40papillary thyroid carcinomamitochondriarespiratory chain complex I |
| spellingShingle | Franz A. Zimmermann Daniel Neureiter Wolfgang Sperl Johannes A. Mayr Barbara Kofler Alterations of Oxidative Phosphorylation Complexes in Papillary Thyroid Carcinoma Cells papillary thyroid carcinoma mitochondria respiratory chain complex I |
| title | Alterations of Oxidative Phosphorylation Complexes in Papillary Thyroid Carcinoma |
| title_full | Alterations of Oxidative Phosphorylation Complexes in Papillary Thyroid Carcinoma |
| title_fullStr | Alterations of Oxidative Phosphorylation Complexes in Papillary Thyroid Carcinoma |
| title_full_unstemmed | Alterations of Oxidative Phosphorylation Complexes in Papillary Thyroid Carcinoma |
| title_short | Alterations of Oxidative Phosphorylation Complexes in Papillary Thyroid Carcinoma |
| title_sort | alterations of oxidative phosphorylation complexes in papillary thyroid carcinoma |
| topic | papillary thyroid carcinoma mitochondria respiratory chain complex I |
| url | http://www.mdpi.com/2073-4409/7/5/40 |
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