Analysis of a Dengue Virus Outbreak in Rosso, Senegal 2021
Senegal is hyperendemic for dengue. Since 2017, outbreaks have been noticed annually in many regions around the country, marked by the co-circulation of DENV1-3. On 8 October 2021, a Dengue virus outbreak in the Rosso health post (sentinel site of the syndromic surveillance network) located in the n...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Tropical Medicine and Infectious Disease |
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| Online Access: | https://www.mdpi.com/2414-6366/7/12/420 |
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| author | Idrissa Dieng Mamadou Aliou Barry Cheikh Talla Bocar Sow Oumar Faye Moussa Moise Diagne Ousseynou Sene Oumar Ndiaye Boly Diop Cheikh Tidiane Diagne Gamou Fall Amadou Alpha Sall Cheikh Loucoubar Ousmane Faye |
| author_facet | Idrissa Dieng Mamadou Aliou Barry Cheikh Talla Bocar Sow Oumar Faye Moussa Moise Diagne Ousseynou Sene Oumar Ndiaye Boly Diop Cheikh Tidiane Diagne Gamou Fall Amadou Alpha Sall Cheikh Loucoubar Ousmane Faye |
| author_sort | Idrissa Dieng |
| collection | DOAJ |
| description | Senegal is hyperendemic for dengue. Since 2017, outbreaks have been noticed annually in many regions around the country, marked by the co-circulation of DENV1-3. On 8 October 2021, a Dengue virus outbreak in the Rosso health post (sentinel site of the syndromic surveillance network) located in the north of the country was notified to the WHO Collaborating Center for arboviruses and hemorrhagic fever viruses at Institut Pasteur de Dakar. A multidisciplinary team was then sent for epidemiological and virologic investigations. This study describes the results from investigations during an outbreak in Senegal using a rapid diagnostic test (RDT) for the combined detection of dengue virus non-structural protein 1 (NS1) and IgM/IgG. For confirmation, samples were also tested by real-time RT-PCR and IgM ELISA at the reference lab in Dakar. qRT-PCR positive samples were subjected to whole genome sequencing using nanopore technology. Virologic analysis scored 102 positives cases (RT-PCR, NS1 antigen detection and/or IgM) out of 173 enrolled patients; interestingly, virus serotyping showed that the outbreak was caused by the DENV-1, a serotype different from DENV-2 involved during the outbreak in Rosso three years earlier, indicating a serotype replacement. Nearly all field-tested NS1 positives samples were confirmed by qRT-PCR with a concordance of 92.3%. Whole genome sequencing and phylogenetic analysis of strains suggested a re-introduction in Rosso of a DENV-1 strain different to the one responsible for the outbreak in the Louga area five years before. Findings call for improved dengue virus surveillance in Senegal, with a wide deployment of DENV antigenic tests, which allow easy on-site diagnosis of suspected cases and early detection of outbreaks. This work highlights the need for continuous monitoring of circulating serotypes which is crucial for a better understanding of viral epidemiology around the country. |
| first_indexed | 2024-03-09T15:47:00Z |
| format | Article |
| id | doaj.art-c18fd9afd92d456492fc6526c4573a18 |
| institution | Directory Open Access Journal |
| issn | 2414-6366 |
| language | English |
| last_indexed | 2024-03-09T15:47:00Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Tropical Medicine and Infectious Disease |
| spelling | doaj.art-c18fd9afd92d456492fc6526c4573a182023-11-24T18:28:17ZengMDPI AGTropical Medicine and Infectious Disease2414-63662022-12-0171242010.3390/tropicalmed7120420Analysis of a Dengue Virus Outbreak in Rosso, Senegal 2021Idrissa Dieng0Mamadou Aliou Barry1Cheikh Talla2Bocar Sow3Oumar Faye4Moussa Moise Diagne5Ousseynou Sene6Oumar Ndiaye7Boly Diop8Cheikh Tidiane Diagne9Gamou Fall10Amadou Alpha Sall11Cheikh Loucoubar12Ousmane Faye13Arboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institute Pasteur de Dakar, Dakar 220, SenegalEpidemiology, Clinical Research and Data Science Department, Institute Pasteur de Dakar, Dakar 220, SenegalEpidemiology, Clinical Research and Data Science Department, Institute Pasteur de Dakar, Dakar 220, SenegalEpidemiology, Clinical Research and Data Science Department, Institute Pasteur de Dakar, Dakar 220, SenegalArboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institute Pasteur de Dakar, Dakar 220, SenegalArboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institute Pasteur de Dakar, Dakar 220, SenegalArboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institute Pasteur de Dakar, Dakar 220, SenegalArboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institute Pasteur de Dakar, Dakar 220, SenegalMinistry of Health, Direction of Prevention, Dakar 220, SenegalArboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institute Pasteur de Dakar, Dakar 220, SenegalArboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institute Pasteur de Dakar, Dakar 220, SenegalArboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institute Pasteur de Dakar, Dakar 220, SenegalEpidemiology, Clinical Research and Data Science Department, Institute Pasteur de Dakar, Dakar 220, SenegalArboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institute Pasteur de Dakar, Dakar 220, SenegalSenegal is hyperendemic for dengue. Since 2017, outbreaks have been noticed annually in many regions around the country, marked by the co-circulation of DENV1-3. On 8 October 2021, a Dengue virus outbreak in the Rosso health post (sentinel site of the syndromic surveillance network) located in the north of the country was notified to the WHO Collaborating Center for arboviruses and hemorrhagic fever viruses at Institut Pasteur de Dakar. A multidisciplinary team was then sent for epidemiological and virologic investigations. This study describes the results from investigations during an outbreak in Senegal using a rapid diagnostic test (RDT) for the combined detection of dengue virus non-structural protein 1 (NS1) and IgM/IgG. For confirmation, samples were also tested by real-time RT-PCR and IgM ELISA at the reference lab in Dakar. qRT-PCR positive samples were subjected to whole genome sequencing using nanopore technology. Virologic analysis scored 102 positives cases (RT-PCR, NS1 antigen detection and/or IgM) out of 173 enrolled patients; interestingly, virus serotyping showed that the outbreak was caused by the DENV-1, a serotype different from DENV-2 involved during the outbreak in Rosso three years earlier, indicating a serotype replacement. Nearly all field-tested NS1 positives samples were confirmed by qRT-PCR with a concordance of 92.3%. Whole genome sequencing and phylogenetic analysis of strains suggested a re-introduction in Rosso of a DENV-1 strain different to the one responsible for the outbreak in the Louga area five years before. Findings call for improved dengue virus surveillance in Senegal, with a wide deployment of DENV antigenic tests, which allow easy on-site diagnosis of suspected cases and early detection of outbreaks. This work highlights the need for continuous monitoring of circulating serotypes which is crucial for a better understanding of viral epidemiology around the country.https://www.mdpi.com/2414-6366/7/12/420DENV-1RossoNS1 RDTsoutbreak responseserotype replacementre-introduction |
| spellingShingle | Idrissa Dieng Mamadou Aliou Barry Cheikh Talla Bocar Sow Oumar Faye Moussa Moise Diagne Ousseynou Sene Oumar Ndiaye Boly Diop Cheikh Tidiane Diagne Gamou Fall Amadou Alpha Sall Cheikh Loucoubar Ousmane Faye Analysis of a Dengue Virus Outbreak in Rosso, Senegal 2021 Tropical Medicine and Infectious Disease DENV-1 Rosso NS1 RDTs outbreak response serotype replacement re-introduction |
| title | Analysis of a Dengue Virus Outbreak in Rosso, Senegal 2021 |
| title_full | Analysis of a Dengue Virus Outbreak in Rosso, Senegal 2021 |
| title_fullStr | Analysis of a Dengue Virus Outbreak in Rosso, Senegal 2021 |
| title_full_unstemmed | Analysis of a Dengue Virus Outbreak in Rosso, Senegal 2021 |
| title_short | Analysis of a Dengue Virus Outbreak in Rosso, Senegal 2021 |
| title_sort | analysis of a dengue virus outbreak in rosso senegal 2021 |
| topic | DENV-1 Rosso NS1 RDTs outbreak response serotype replacement re-introduction |
| url | https://www.mdpi.com/2414-6366/7/12/420 |
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