Prevalence and distribution of subtypes of Blastocystis in Asiatic brush-tailed porcupines (Atherurus macrourus), bamboo rats (Rhizomys pruinosus), and masked palm civets (Paguma larvata) farmed in Hainan, China
Blastocystis sp. is an important gastrointestinal parasite with global distribution, prevalent in humans, farmed animals, and wildlife. Therefore, this study aimed to investigate the prevalence and genetic diversity of Blastocystis sp. in Asiatic brush-tailed porcupines (Atherurus macrourus), bamboo...
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EDP Sciences
2023-01-01
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| Series: | Parasite |
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| Online Access: | https://www.parasite-journal.org/articles/parasite/full_html/2023/01/parasite230128/parasite230128.html |
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| author | Zhao Wei Zhang Yun Li Jiaqi Ren Guangxu Qiang Yu Wang Yuan Lai Xiuyi Lei Sheng Liu Rui Chen Yuankun Huang Huicong Li Wenting Lu Gang Tan Feng |
| author_facet | Zhao Wei Zhang Yun Li Jiaqi Ren Guangxu Qiang Yu Wang Yuan Lai Xiuyi Lei Sheng Liu Rui Chen Yuankun Huang Huicong Li Wenting Lu Gang Tan Feng |
| author_sort | Zhao Wei |
| collection | DOAJ |
| description | Blastocystis sp. is an important gastrointestinal parasite with global distribution, prevalent in humans, farmed animals, and wildlife. Therefore, this study aimed to investigate the prevalence and genetic diversity of Blastocystis sp. in Asiatic brush-tailed porcupines (Atherurus macrourus), bamboo rats (Rhizomys pruinosus), and masked palm civets (Paguma larvata) in Hainan Province, China. A total of 900 fecal samples were collected from three farmed animal species including 257 porcupines, 360 rats, and 283 civets. Genomic DNA was extracted from each fecal sample and Blastocystis sp. was detected by PCR at the small subunit ribosomal RNA (SSU rRNA) gene. A phylogenetic tree was constructed using the maximum likelihood method. Blastocystis sp. was detected in 47 (5.2%) fecal samples: 12 (4.7%) Asiatic brush-tailed porcupines, 8 (2.2%) bamboo rats, and 27 (9.5%) masked palm civets. Three known Blastocystis sp. subtypes, including ST1, ST4, ST5, and one unnamed subtype (unST), were found in one, 19, 26, and one animal, respectively. Subtypes ST4 and unST were detected in porcupines, ST4 in rats, and ST1 and ST5 in civets. Our results suggest that the three farmed animal species reported in this study could serve as reservoirs for potentially zoonotic Blastocystis sp. subtypes and transmit this parasite to humans, other farmed animals, and wildlife. |
| first_indexed | 2024-03-11T12:13:04Z |
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| language | English |
| last_indexed | 2024-03-11T12:13:04Z |
| publishDate | 2023-01-01 |
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| series | Parasite |
| spelling | doaj.art-c1946b12c33f433993be420670bdebdf2023-11-07T10:39:54ZengEDP SciencesParasite1776-10422023-01-01304510.1051/parasite/2023048parasite230128Prevalence and distribution of subtypes of Blastocystis in Asiatic brush-tailed porcupines (Atherurus macrourus), bamboo rats (Rhizomys pruinosus), and masked palm civets (Paguma larvata) farmed in Hainan, ChinaZhao Wei0Zhang Yun1Li Jiaqi2Ren Guangxu3Qiang Yu4Wang Yuan5Lai Xiuyi6Lei Sheng7Liu Rui8Chen Yuankun9Huang Huicong10Li Wenting11Lu Gang12Tan Feng13Department of Parasitology, Wenzhou Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Department of Pathogenic Biology, Hainan Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Department of Pathogenic Biology, Hainan Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Department of Pathogenic Biology, Hainan Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Department of Pathogenic Biology, Hainan Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Department of Pathogenic Biology, Hainan Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Department of Pathogenic Biology, Hainan Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Department of Pathogenic Biology, Hainan Medical UniversityDepartment of Infectious and Tropical Diseases, the Second Affiliated Hospital of Hainan Medical UniversityDepartment of Infectious and Tropical Diseases, the Second Affiliated Hospital of Hainan Medical UniversityDepartment of Parasitology, Wenzhou Medical UniversityDepartment of Infectious and Tropical Diseases, the Second Affiliated Hospital of Hainan Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Department of Pathogenic Biology, Hainan Medical UniversityDepartment of Parasitology, Wenzhou Medical UniversityBlastocystis sp. is an important gastrointestinal parasite with global distribution, prevalent in humans, farmed animals, and wildlife. Therefore, this study aimed to investigate the prevalence and genetic diversity of Blastocystis sp. in Asiatic brush-tailed porcupines (Atherurus macrourus), bamboo rats (Rhizomys pruinosus), and masked palm civets (Paguma larvata) in Hainan Province, China. A total of 900 fecal samples were collected from three farmed animal species including 257 porcupines, 360 rats, and 283 civets. Genomic DNA was extracted from each fecal sample and Blastocystis sp. was detected by PCR at the small subunit ribosomal RNA (SSU rRNA) gene. A phylogenetic tree was constructed using the maximum likelihood method. Blastocystis sp. was detected in 47 (5.2%) fecal samples: 12 (4.7%) Asiatic brush-tailed porcupines, 8 (2.2%) bamboo rats, and 27 (9.5%) masked palm civets. Three known Blastocystis sp. subtypes, including ST1, ST4, ST5, and one unnamed subtype (unST), were found in one, 19, 26, and one animal, respectively. Subtypes ST4 and unST were detected in porcupines, ST4 in rats, and ST1 and ST5 in civets. Our results suggest that the three farmed animal species reported in this study could serve as reservoirs for potentially zoonotic Blastocystis sp. subtypes and transmit this parasite to humans, other farmed animals, and wildlife.https://www.parasite-journal.org/articles/parasite/full_html/2023/01/parasite230128/parasite230128.htmlblastocystis sp.zoonoticsubtypesfarm animalshainan (china) |
| spellingShingle | Zhao Wei Zhang Yun Li Jiaqi Ren Guangxu Qiang Yu Wang Yuan Lai Xiuyi Lei Sheng Liu Rui Chen Yuankun Huang Huicong Li Wenting Lu Gang Tan Feng Prevalence and distribution of subtypes of Blastocystis in Asiatic brush-tailed porcupines (Atherurus macrourus), bamboo rats (Rhizomys pruinosus), and masked palm civets (Paguma larvata) farmed in Hainan, China Parasite blastocystis sp. zoonotic subtypes farm animals hainan (china) |
| title | Prevalence and distribution of subtypes of Blastocystis in Asiatic brush-tailed porcupines (Atherurus macrourus), bamboo rats (Rhizomys pruinosus), and masked palm civets (Paguma larvata) farmed in Hainan, China |
| title_full | Prevalence and distribution of subtypes of Blastocystis in Asiatic brush-tailed porcupines (Atherurus macrourus), bamboo rats (Rhizomys pruinosus), and masked palm civets (Paguma larvata) farmed in Hainan, China |
| title_fullStr | Prevalence and distribution of subtypes of Blastocystis in Asiatic brush-tailed porcupines (Atherurus macrourus), bamboo rats (Rhizomys pruinosus), and masked palm civets (Paguma larvata) farmed in Hainan, China |
| title_full_unstemmed | Prevalence and distribution of subtypes of Blastocystis in Asiatic brush-tailed porcupines (Atherurus macrourus), bamboo rats (Rhizomys pruinosus), and masked palm civets (Paguma larvata) farmed in Hainan, China |
| title_short | Prevalence and distribution of subtypes of Blastocystis in Asiatic brush-tailed porcupines (Atherurus macrourus), bamboo rats (Rhizomys pruinosus), and masked palm civets (Paguma larvata) farmed in Hainan, China |
| title_sort | prevalence and distribution of subtypes of blastocystis in asiatic brush tailed porcupines atherurus macrourus bamboo rats rhizomys pruinosus and masked palm civets paguma larvata farmed in hainan china |
| topic | blastocystis sp. zoonotic subtypes farm animals hainan (china) |
| url | https://www.parasite-journal.org/articles/parasite/full_html/2023/01/parasite230128/parasite230128.html |
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