Characterizing Mobilized Virulence Factors and Multidrug Resistance Genes in Carbapenemase-Producing Klebsiella pneumoniae in a Sri Lankan Hospital
Limited data is available on the epidemiology and characteristics of carbapenem-resistant Enterobacteriaceae (CRE) and their associated plasmids or virulence determinants from Sri Lanka. Through whole genome sequencing of CREs from the intensive care units of a Sri Lankan teaching hospital, we ident...
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| Format: | Article |
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Frontiers Media S.A.
2018-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02044/full |
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| author | Chendi Zhu Veranja Liyanapathirana Carmen Li Vasanthi Pinto Mamie Hui Norman Lo Kam T. Wong Nilanthi Dissanayake Margaret Ip |
| author_facet | Chendi Zhu Veranja Liyanapathirana Carmen Li Vasanthi Pinto Mamie Hui Norman Lo Kam T. Wong Nilanthi Dissanayake Margaret Ip |
| author_sort | Chendi Zhu |
| collection | DOAJ |
| description | Limited data is available on the epidemiology and characteristics of carbapenem-resistant Enterobacteriaceae (CRE) and their associated plasmids or virulence determinants from Sri Lanka. Through whole genome sequencing of CREs from the intensive care units of a Sri Lankan teaching hospital, we identified a carbapenemase gene, blaOXA–181 in 10 carbapenemase-producing Klebsiella pneumoniae isolates (two strains of ST437 and eight strains of ST147) from 379 respiratory specimens. blaOXA–181 was carried in three variants of ColE-type plasmids. K. pneumoniae strains with ompK36 variants showed high minimum inhibitory concentrations to carbapenem. Furthermore, genes encoding for extended spectrum β-lactamases (ESBL), plasmid-mediated quinolone resistance (PMQR) determinants (qnr, aac(6′)-Ib-cr, and oqxAB) were present in all 10 strains. Amino acid substitution in chromosomal quinolone resistance-determining regions (QRDRs) gyrA (Ser83Ile) and parC (Ser80Ile) were also observed. All strains had yersiniabactin genes on mobile element ICEkp. Strict infection control practices and judicious use of antibiotics are warranted to prevent further spread of multidrug-resistant K. pneumoniae. |
| first_indexed | 2024-12-13T18:46:17Z |
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| id | doaj.art-c195912d973b480d811db3ea0d9b1bbf |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T18:46:17Z |
| publishDate | 2018-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c195912d973b480d811db3ea0d9b1bbf2022-12-21T23:35:04ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-08-01910.3389/fmicb.2018.02044408038Characterizing Mobilized Virulence Factors and Multidrug Resistance Genes in Carbapenemase-Producing Klebsiella pneumoniae in a Sri Lankan HospitalChendi Zhu0Veranja Liyanapathirana1Carmen Li2Vasanthi Pinto3Mamie Hui4Norman Lo5Kam T. Wong6Nilanthi Dissanayake7Margaret Ip8Department of Microbiology, The Chinese University of Hong Kong, Shatin, Hong KongDepartment of Microbiology, Faculty of Medicine, University of Peradeniya, Kandy, Sri LankaDepartment of Microbiology, The Chinese University of Hong Kong, Shatin, Hong KongDepartment of Anesthesiology and Critical Care, Faculty of Medicine, University of Peradeniya, Kandy, Sri LankaDepartment of Microbiology, The Chinese University of Hong Kong, Shatin, Hong KongDepartment of Microbiology, The Chinese University of Hong Kong, Shatin, Hong KongDepartment of Microbiology, The Chinese University of Hong Kong, Shatin, Hong KongDepartment of Microbiology, Faculty of Medicine, University of Peradeniya, Kandy, Sri LankaDepartment of Microbiology, The Chinese University of Hong Kong, Shatin, Hong KongLimited data is available on the epidemiology and characteristics of carbapenem-resistant Enterobacteriaceae (CRE) and their associated plasmids or virulence determinants from Sri Lanka. Through whole genome sequencing of CREs from the intensive care units of a Sri Lankan teaching hospital, we identified a carbapenemase gene, blaOXA–181 in 10 carbapenemase-producing Klebsiella pneumoniae isolates (two strains of ST437 and eight strains of ST147) from 379 respiratory specimens. blaOXA–181 was carried in three variants of ColE-type plasmids. K. pneumoniae strains with ompK36 variants showed high minimum inhibitory concentrations to carbapenem. Furthermore, genes encoding for extended spectrum β-lactamases (ESBL), plasmid-mediated quinolone resistance (PMQR) determinants (qnr, aac(6′)-Ib-cr, and oqxAB) were present in all 10 strains. Amino acid substitution in chromosomal quinolone resistance-determining regions (QRDRs) gyrA (Ser83Ile) and parC (Ser80Ile) were also observed. All strains had yersiniabactin genes on mobile element ICEkp. Strict infection control practices and judicious use of antibiotics are warranted to prevent further spread of multidrug-resistant K. pneumoniae.https://www.frontiersin.org/article/10.3389/fmicb.2018.02044/fullOXA-181quinoloneyersiniabactinKlebsiella pneumoniaeSri Lanka |
| spellingShingle | Chendi Zhu Veranja Liyanapathirana Carmen Li Vasanthi Pinto Mamie Hui Norman Lo Kam T. Wong Nilanthi Dissanayake Margaret Ip Characterizing Mobilized Virulence Factors and Multidrug Resistance Genes in Carbapenemase-Producing Klebsiella pneumoniae in a Sri Lankan Hospital Frontiers in Microbiology OXA-181 quinolone yersiniabactin Klebsiella pneumoniae Sri Lanka |
| title | Characterizing Mobilized Virulence Factors and Multidrug Resistance Genes in Carbapenemase-Producing Klebsiella pneumoniae in a Sri Lankan Hospital |
| title_full | Characterizing Mobilized Virulence Factors and Multidrug Resistance Genes in Carbapenemase-Producing Klebsiella pneumoniae in a Sri Lankan Hospital |
| title_fullStr | Characterizing Mobilized Virulence Factors and Multidrug Resistance Genes in Carbapenemase-Producing Klebsiella pneumoniae in a Sri Lankan Hospital |
| title_full_unstemmed | Characterizing Mobilized Virulence Factors and Multidrug Resistance Genes in Carbapenemase-Producing Klebsiella pneumoniae in a Sri Lankan Hospital |
| title_short | Characterizing Mobilized Virulence Factors and Multidrug Resistance Genes in Carbapenemase-Producing Klebsiella pneumoniae in a Sri Lankan Hospital |
| title_sort | characterizing mobilized virulence factors and multidrug resistance genes in carbapenemase producing klebsiella pneumoniae in a sri lankan hospital |
| topic | OXA-181 quinolone yersiniabactin Klebsiella pneumoniae Sri Lanka |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.02044/full |
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