Engineering mesophilic GH11 xylanase from Cellulomonas flavigena by rational design of N-terminus substitution

Xylanase, a glycoside hydrolase, is widely used in the food, papermaking, and textile industries; however, most xylanases are inactive at high temperatures. In this study, a xylanase gene, CFXyl3, was cloned from Cellulomonas flavigena and expressed in Escherichia coli BL21 (DE3). To improve the the...

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Bibliographic Details
Main Authors: Wenzhuo Tian, Ziyang Zhang, Cuiping Yang, Piwu Li, Jing Xiao, Ruiming Wang, Peng Du, Nan Li, Junqing Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-11-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2022.1044291/full