Preparation and structural analysis of fucomannogalactan and β-1,6-glucan from Grifola frondosa mycelium
Introduction: Polysaccharides, key components present in Grifola frondosa, can be divided into those derived from fruiting bodies, mycelium, and fermentation broth based on their source. The structure of G. frondosa fruiting body-derived polysaccharides has been fully characterized. However, the str...
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2023.1227288/full |
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| author | Jie Geng Guining Wang Jiao Guo Xiao Han Yunhe Qu Yifa Zhou Guihua Tai Lin Sun Hairong Cheng |
| author_facet | Jie Geng Guining Wang Jiao Guo Xiao Han Yunhe Qu Yifa Zhou Guihua Tai Lin Sun Hairong Cheng |
| author_sort | Jie Geng |
| collection | DOAJ |
| description | Introduction: Polysaccharides, key components present in Grifola frondosa, can be divided into those derived from fruiting bodies, mycelium, and fermentation broth based on their source. The structure of G. frondosa fruiting body-derived polysaccharides has been fully characterized. However, the structure of G. frondosa mycelium-derived polysaccharides remains to be elucidated.Methods: In this study, we obtained mycelia from G. frondosa by liquid fermentation and extracted them with water and alkaline solution. Then, the mycelia were isolated and purified to obtain homogeneity and systematically characterized by methylation and FT infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopy.Results and discussion: Structural analysis showed that two neutral fractions (WGFP-N-a and AGFP-N-a1) have a common backbone composed of α-1,6-D-Me-Galp and α-1,6-D-Galp that were substituted at O-2 by 1,2-Manp, α-1,3-L-Fucp, and α-T-D-Manp and thus are identified as fucomannogalactans. WGFP-A-a, AGFP-A-b, and AGFP-A-c are β-1,6-glucans with different molecular weights and are branched with β-1,3-D-Glcp and T-D-Glcp at the O-3 of Glc. Our results provide important structural information about G. frondosa mycelium-derived polysaccharides and provide the basis for their further development and application. |
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| last_indexed | 2024-03-12T17:00:08Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Chemistry |
| spelling | doaj.art-c197389462724fd084c075ae13ddc9412023-08-07T17:54:45ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462023-08-011110.3389/fchem.2023.12272881227288Preparation and structural analysis of fucomannogalactan and β-1,6-glucan from Grifola frondosa myceliumJie Geng0Guining Wang1Jiao Guo2Xiao Han3Yunhe Qu4Yifa Zhou5Guihua Tai6Lin Sun7Hairong Cheng8Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, Glycoconjugate Engineering Research Center of the Ministry of Education, School of Life Sciences, Northeast Normal University, Changchun, ChinaJilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, Glycoconjugate Engineering Research Center of the Ministry of Education, School of Life Sciences, Northeast Normal University, Changchun, ChinaJilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, Glycoconjugate Engineering Research Center of the Ministry of Education, School of Life Sciences, Northeast Normal University, Changchun, ChinaJilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, Glycoconjugate Engineering Research Center of the Ministry of Education, School of Life Sciences, Northeast Normal University, Changchun, ChinaCentral Laboratory, Changchun Normal University, Changchun, ChinaJilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, Glycoconjugate Engineering Research Center of the Ministry of Education, School of Life Sciences, Northeast Normal University, Changchun, ChinaJilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, Glycoconjugate Engineering Research Center of the Ministry of Education, School of Life Sciences, Northeast Normal University, Changchun, ChinaJilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, Glycoconjugate Engineering Research Center of the Ministry of Education, School of Life Sciences, Northeast Normal University, Changchun, ChinaJilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, Glycoconjugate Engineering Research Center of the Ministry of Education, School of Life Sciences, Northeast Normal University, Changchun, ChinaIntroduction: Polysaccharides, key components present in Grifola frondosa, can be divided into those derived from fruiting bodies, mycelium, and fermentation broth based on their source. The structure of G. frondosa fruiting body-derived polysaccharides has been fully characterized. However, the structure of G. frondosa mycelium-derived polysaccharides remains to be elucidated.Methods: In this study, we obtained mycelia from G. frondosa by liquid fermentation and extracted them with water and alkaline solution. Then, the mycelia were isolated and purified to obtain homogeneity and systematically characterized by methylation and FT infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopy.Results and discussion: Structural analysis showed that two neutral fractions (WGFP-N-a and AGFP-N-a1) have a common backbone composed of α-1,6-D-Me-Galp and α-1,6-D-Galp that were substituted at O-2 by 1,2-Manp, α-1,3-L-Fucp, and α-T-D-Manp and thus are identified as fucomannogalactans. WGFP-A-a, AGFP-A-b, and AGFP-A-c are β-1,6-glucans with different molecular weights and are branched with β-1,3-D-Glcp and T-D-Glcp at the O-3 of Glc. Our results provide important structural information about G. frondosa mycelium-derived polysaccharides and provide the basis for their further development and application.https://www.frontiersin.org/articles/10.3389/fchem.2023.1227288/fullGrifola frondosamyceliumfucomannogalactanglucanstructure analysis |
| spellingShingle | Jie Geng Guining Wang Jiao Guo Xiao Han Yunhe Qu Yifa Zhou Guihua Tai Lin Sun Hairong Cheng Preparation and structural analysis of fucomannogalactan and β-1,6-glucan from Grifola frondosa mycelium Frontiers in Chemistry Grifola frondosa mycelium fucomannogalactan glucan structure analysis |
| title | Preparation and structural analysis of fucomannogalactan and β-1,6-glucan from Grifola frondosa mycelium |
| title_full | Preparation and structural analysis of fucomannogalactan and β-1,6-glucan from Grifola frondosa mycelium |
| title_fullStr | Preparation and structural analysis of fucomannogalactan and β-1,6-glucan from Grifola frondosa mycelium |
| title_full_unstemmed | Preparation and structural analysis of fucomannogalactan and β-1,6-glucan from Grifola frondosa mycelium |
| title_short | Preparation and structural analysis of fucomannogalactan and β-1,6-glucan from Grifola frondosa mycelium |
| title_sort | preparation and structural analysis of fucomannogalactan and β 1 6 glucan from grifola frondosa mycelium |
| topic | Grifola frondosa mycelium fucomannogalactan glucan structure analysis |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2023.1227288/full |
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