Evaluation of the doxycycline release from AH26 sealer-doxycycline combination: An ex vivo study
INTRODUCTION: The purpose of this ex vivo study was to determine the releasing characteristics and doxycycline dentinal diffusion of AH26 sealer-doxycycline combination from apical 3mm of tooth root and apical foramen. MATERIALS & METHODS: One-hundred and two recently extracted single-rooted hum...
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| Format: | Article |
| Language: | English |
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Iranian Association of Endodontists; Iranian Center for Endodontic Research
2011-09-01
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| Series: | Iranian Endodontic Journal |
| Subjects: | |
| Online Access: | http://journals.sbmu.ac.ir/index.php/iej/article/view/2459/2228 |
| _version_ | 1817970094017347584 |
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| author | Kazem Ashofteh Yazdi Noushin Shokouhinejad Esmaeil Moazeni Sina Mirzayi Rad |
| author_facet | Kazem Ashofteh Yazdi Noushin Shokouhinejad Esmaeil Moazeni Sina Mirzayi Rad |
| author_sort | Kazem Ashofteh Yazdi |
| collection | DOAJ |
| description | INTRODUCTION: The purpose of this ex vivo study was to determine the releasing characteristics and doxycycline dentinal diffusion of AH26 sealer-doxycycline combination from apical 3mm of tooth root and apical foramen. MATERIALS & METHODS: One-hundred and two recently extracted single-rooted human teeth were decoronated and prepared with #3 and #4 Gates-Glidden drills and rotary Mtwo files. Smear layer was removed; all surfaces except for apical 3mm of each root were sealed with two coats of nail polish. To quantify the release and diffusion of the doxycycline at different time intervals (30 min, 48 and 72 h) after root canal obturation, the samples were randomly divided into three groups (n=30; 0.5 h, 48 h, 72 h). To evaluate the release of doxycycline from AH26 sealer-doxycycline combination at six concentrations of antibiotic including 0.5%, 1%, 2%, 5%, 10% and 20%; each experimental group was divided into six equal subgroups (n=5). Root canals were filled with gutta-percha and AH26-doxycycline combinations and then were placed in vials containing 1.25mL of phosphate buffer saline solution (PBS). After 30 min, 48 and 72 h, the amount of doxycycline released from specimens into PBS were determined by measuring the absorbance values using UV spectrophotometry at λmax=350 nm. Data were analyzed using two-way ANOVA. RESULTS: The findings of this study revealed that AH26 sealer-doxycycline combination released variable measures of antibiotic at each time interval and in the various concentrations. At 30 min, no statistically significant differences were obtained between the results of subgroups, but at 48 and 72 h these differences were significant (P < 0.001). The results also showed that differences between 0.5 h, 48 h and 72 h were significant within subgroups (P < 0.01). CONCLUSION: Under the conditions of this ex vivo study, doxycycline can be released from AH26 sealer-antibiotic combination through 3mm of apical root and apical foramen at 30 min, 48 and 72 h after mixing the sealer with doxycycline at concentrations of 0.5% up to 20%. |
| first_indexed | 2024-04-13T20:29:46Z |
| format | Article |
| id | doaj.art-c1977b01832b418c948d9f7296aa3ee6 |
| institution | Directory Open Access Journal |
| issn | 1735-7497 2008-2746 |
| language | English |
| last_indexed | 2024-04-13T20:29:46Z |
| publishDate | 2011-09-01 |
| publisher | Iranian Association of Endodontists; Iranian Center for Endodontic Research |
| record_format | Article |
| series | Iranian Endodontic Journal |
| spelling | doaj.art-c1977b01832b418c948d9f7296aa3ee62022-12-22T02:31:12ZengIranian Association of Endodontists; Iranian Center for Endodontic ResearchIranian Endodontic Journal1735-74972008-27462011-09-0164160167Evaluation of the doxycycline release from AH26 sealer-doxycycline combination: An ex vivo studyKazem Ashofteh YazdiNoushin ShokouhinejadEsmaeil MoazeniSina Mirzayi RadINTRODUCTION: The purpose of this ex vivo study was to determine the releasing characteristics and doxycycline dentinal diffusion of AH26 sealer-doxycycline combination from apical 3mm of tooth root and apical foramen. MATERIALS & METHODS: One-hundred and two recently extracted single-rooted human teeth were decoronated and prepared with #3 and #4 Gates-Glidden drills and rotary Mtwo files. Smear layer was removed; all surfaces except for apical 3mm of each root were sealed with two coats of nail polish. To quantify the release and diffusion of the doxycycline at different time intervals (30 min, 48 and 72 h) after root canal obturation, the samples were randomly divided into three groups (n=30; 0.5 h, 48 h, 72 h). To evaluate the release of doxycycline from AH26 sealer-doxycycline combination at six concentrations of antibiotic including 0.5%, 1%, 2%, 5%, 10% and 20%; each experimental group was divided into six equal subgroups (n=5). Root canals were filled with gutta-percha and AH26-doxycycline combinations and then were placed in vials containing 1.25mL of phosphate buffer saline solution (PBS). After 30 min, 48 and 72 h, the amount of doxycycline released from specimens into PBS were determined by measuring the absorbance values using UV spectrophotometry at λmax=350 nm. Data were analyzed using two-way ANOVA. RESULTS: The findings of this study revealed that AH26 sealer-doxycycline combination released variable measures of antibiotic at each time interval and in the various concentrations. At 30 min, no statistically significant differences were obtained between the results of subgroups, but at 48 and 72 h these differences were significant (P < 0.001). The results also showed that differences between 0.5 h, 48 h and 72 h were significant within subgroups (P < 0.01). CONCLUSION: Under the conditions of this ex vivo study, doxycycline can be released from AH26 sealer-antibiotic combination through 3mm of apical root and apical foramen at 30 min, 48 and 72 h after mixing the sealer with doxycycline at concentrations of 0.5% up to 20%.http://journals.sbmu.ac.ir/index.php/iej/article/view/2459/2228AH26 sealerDoxycyclineRoot canal |
| spellingShingle | Kazem Ashofteh Yazdi Noushin Shokouhinejad Esmaeil Moazeni Sina Mirzayi Rad Evaluation of the doxycycline release from AH26 sealer-doxycycline combination: An ex vivo study Iranian Endodontic Journal AH26 sealer Doxycycline Root canal |
| title | Evaluation of the doxycycline release from AH26 sealer-doxycycline combination: An ex vivo study |
| title_full | Evaluation of the doxycycline release from AH26 sealer-doxycycline combination: An ex vivo study |
| title_fullStr | Evaluation of the doxycycline release from AH26 sealer-doxycycline combination: An ex vivo study |
| title_full_unstemmed | Evaluation of the doxycycline release from AH26 sealer-doxycycline combination: An ex vivo study |
| title_short | Evaluation of the doxycycline release from AH26 sealer-doxycycline combination: An ex vivo study |
| title_sort | evaluation of the doxycycline release from ah26 sealer doxycycline combination an ex vivo study |
| topic | AH26 sealer Doxycycline Root canal |
| url | http://journals.sbmu.ac.ir/index.php/iej/article/view/2459/2228 |
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