Colorectal Cancer Cells Adhere to and Migrate Along the Neurons of the Enteric Nervous System
In several types of cancers, tumor cells invade adjacent tissues by migrating along the resident nerves of the tumor microenvironment. This process, called perineural invasion, typically occurs along extrinsic nerves, with Schwann cells providing physical guidance for the tumor cells. However, in th...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-01-01
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| Series: | Cellular and Molecular Gastroenterology and Hepatology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352345X17301480 |
| _version_ | 1811304986366181376 |
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| author | Emilie Duchalais Christophe Guilluy Steven Nedellec Melissa Touvron Anne Bessard Yann Touchefeu Céline Bossard Hélène Boudin Guy Louarn Michel Neunlist Laurianne Van Landeghem |
| author_facet | Emilie Duchalais Christophe Guilluy Steven Nedellec Melissa Touvron Anne Bessard Yann Touchefeu Céline Bossard Hélène Boudin Guy Louarn Michel Neunlist Laurianne Van Landeghem |
| author_sort | Emilie Duchalais |
| collection | DOAJ |
| description | In several types of cancers, tumor cells invade adjacent tissues by migrating along the resident nerves of the tumor microenvironment. This process, called perineural invasion, typically occurs along extrinsic nerves, with Schwann cells providing physical guidance for the tumor cells. However, in the colorectal cancer microenvironment, the most abundant nervous structures belong to the nonmyelinated intrinsic enteric nervous system (ENS). In this study, we investigated whether colon cancer cells interact with the ENS.
Methods: Tumor epithelial cells (TECs) from human primary colon adenocarcinomas and cell lines were cocultured with primary cultures of ENS and cultures of human ENS plexus explants. By combining confocal and atomic force microscopy, as well as video microscopy, we assessed tumor cell adhesion and migration on the ENS. We identified the adhesion proteins involved using a proteomics approach based on biotin/streptavidin interaction, and their implication was confirmed further using selective blocking antibodies.
Results: TEC adhered preferentially and with stronger adhesion forces to enteric nervous structures than to mesenchymal cells. TEC adhesion to ENS involved direct interactions with enteric neurons. Enteric neuron removal from ENS cultures led to a significant decrease in tumor cell adhesion. TECs migrated significantly longer and further when adherent on ENS compared with on mesenchymal cells, and their trajectory faithfully followed ENS structures. Blocking N-cadherin and L1CAM decreased TEC migration along ENS structures.
Conclusions: Our data show that the enteric neuronal network guides tumor cell migration, partly via L1CAM and N-cadherin. These results open a new avenue of research on the underlying mechanisms and consequences of perineural invasion in colorectal cancer. |
| first_indexed | 2024-04-13T08:17:13Z |
| format | Article |
| id | doaj.art-c1a241bb18b04076b030fcd1822be9a5 |
| institution | Directory Open Access Journal |
| issn | 2352-345X |
| language | English |
| last_indexed | 2024-04-13T08:17:13Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cellular and Molecular Gastroenterology and Hepatology |
| spelling | doaj.art-c1a241bb18b04076b030fcd1822be9a52022-12-22T02:54:44ZengElsevierCellular and Molecular Gastroenterology and Hepatology2352-345X2018-01-0151314910.1016/j.jcmgh.2017.10.002Colorectal Cancer Cells Adhere to and Migrate Along the Neurons of the Enteric Nervous SystemEmilie Duchalais0Christophe Guilluy1Steven Nedellec2Melissa Touvron3Anne Bessard4Yann Touchefeu5Céline Bossard6Hélène Boudin7Guy Louarn8Michel Neunlist9Laurianne Van Landeghem10Inserm U1235, Institut des Maladies de l'Appareil Digestif, Nantes, FranceUniversité de Nantes, Nantes, FranceUniversité de Nantes, Nantes, FranceInserm U1235, Institut des Maladies de l'Appareil Digestif, Nantes, FranceInserm U1235, Institut des Maladies de l'Appareil Digestif, Nantes, FranceInserm U1235, Institut des Maladies de l'Appareil Digestif, Nantes, FranceUniversité de Nantes, Nantes, FranceInserm U1235, Institut des Maladies de l'Appareil Digestif, Nantes, FranceUniversité de Nantes, Nantes, FranceInserm U1235, Institut des Maladies de l'Appareil Digestif, Nantes, FranceInserm U1235, Institut des Maladies de l'Appareil Digestif, Nantes, FranceIn several types of cancers, tumor cells invade adjacent tissues by migrating along the resident nerves of the tumor microenvironment. This process, called perineural invasion, typically occurs along extrinsic nerves, with Schwann cells providing physical guidance for the tumor cells. However, in the colorectal cancer microenvironment, the most abundant nervous structures belong to the nonmyelinated intrinsic enteric nervous system (ENS). In this study, we investigated whether colon cancer cells interact with the ENS. Methods: Tumor epithelial cells (TECs) from human primary colon adenocarcinomas and cell lines were cocultured with primary cultures of ENS and cultures of human ENS plexus explants. By combining confocal and atomic force microscopy, as well as video microscopy, we assessed tumor cell adhesion and migration on the ENS. We identified the adhesion proteins involved using a proteomics approach based on biotin/streptavidin interaction, and their implication was confirmed further using selective blocking antibodies. Results: TEC adhered preferentially and with stronger adhesion forces to enteric nervous structures than to mesenchymal cells. TEC adhesion to ENS involved direct interactions with enteric neurons. Enteric neuron removal from ENS cultures led to a significant decrease in tumor cell adhesion. TECs migrated significantly longer and further when adherent on ENS compared with on mesenchymal cells, and their trajectory faithfully followed ENS structures. Blocking N-cadherin and L1CAM decreased TEC migration along ENS structures. Conclusions: Our data show that the enteric neuronal network guides tumor cell migration, partly via L1CAM and N-cadherin. These results open a new avenue of research on the underlying mechanisms and consequences of perineural invasion in colorectal cancer.http://www.sciencedirect.com/science/article/pii/S2352345X17301480Colorectal CancerEnteric NeuronsAdhesionMigration |
| spellingShingle | Emilie Duchalais Christophe Guilluy Steven Nedellec Melissa Touvron Anne Bessard Yann Touchefeu Céline Bossard Hélène Boudin Guy Louarn Michel Neunlist Laurianne Van Landeghem Colorectal Cancer Cells Adhere to and Migrate Along the Neurons of the Enteric Nervous System Cellular and Molecular Gastroenterology and Hepatology Colorectal Cancer Enteric Neurons Adhesion Migration |
| title | Colorectal Cancer Cells Adhere to and Migrate Along the Neurons of the Enteric Nervous System |
| title_full | Colorectal Cancer Cells Adhere to and Migrate Along the Neurons of the Enteric Nervous System |
| title_fullStr | Colorectal Cancer Cells Adhere to and Migrate Along the Neurons of the Enteric Nervous System |
| title_full_unstemmed | Colorectal Cancer Cells Adhere to and Migrate Along the Neurons of the Enteric Nervous System |
| title_short | Colorectal Cancer Cells Adhere to and Migrate Along the Neurons of the Enteric Nervous System |
| title_sort | colorectal cancer cells adhere to and migrate along the neurons of the enteric nervous system |
| topic | Colorectal Cancer Enteric Neurons Adhesion Migration |
| url | http://www.sciencedirect.com/science/article/pii/S2352345X17301480 |
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