A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain
Imaging the expression patterns of reporter constructs is a powerful tool to dissect the neuronal circuits of perception and behavior in the adult brain of Drosophila, one of the major models for studying brain functions. To date, several Drosophila brain templates and digital atlases have been buil...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-03-01
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| Series: | Frontiers in Neuroinformatics |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fninf.2018.00013/full |
| _version_ | 1818624559432073216 |
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| author | Ignacio Arganda-Carreras Ignacio Arganda-Carreras Ignacio Arganda-Carreras Tudor Manoliu Nicolas Mazuras Florian Schulze Juan E. Iglesias Katja Bühler Arnim Jenett François Rouyer Philippe Andrey |
| author_facet | Ignacio Arganda-Carreras Ignacio Arganda-Carreras Ignacio Arganda-Carreras Tudor Manoliu Nicolas Mazuras Florian Schulze Juan E. Iglesias Katja Bühler Arnim Jenett François Rouyer Philippe Andrey |
| author_sort | Ignacio Arganda-Carreras |
| collection | DOAJ |
| description | Imaging the expression patterns of reporter constructs is a powerful tool to dissect the neuronal circuits of perception and behavior in the adult brain of Drosophila, one of the major models for studying brain functions. To date, several Drosophila brain templates and digital atlases have been built to automatically analyze and compare collections of expression pattern images. However, there has been no systematic comparison of performances between alternative atlasing strategies and registration algorithms. Here, we objectively evaluated the performance of different strategies for building adult Drosophila brain templates and atlases. In addition, we used state-of-the-art registration algorithms to generate a new group-wise inter-sex atlas. Our results highlight the benefit of statistical atlases over individual ones and show that the newly proposed inter-sex atlas outperformed existing solutions for automated registration and annotation of expression patterns. Over 3,000 images from the Janelia Farm FlyLight collection were registered using the proposed strategy. These registered expression patterns can be searched and compared with a new version of the BrainBaseWeb system and BrainGazer software. We illustrate the validity of our methodology and brain atlas with registration-based predictions of expression patterns in a subset of clock neurons. The described registration framework should benefit to brain studies in Drosophila and other insect species. |
| first_indexed | 2024-12-16T18:58:53Z |
| format | Article |
| id | doaj.art-c1a78a32223e4a7dbc7b80b305226a2d |
| institution | Directory Open Access Journal |
| issn | 1662-5196 |
| language | English |
| last_indexed | 2024-12-16T18:58:53Z |
| publishDate | 2018-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroinformatics |
| spelling | doaj.art-c1a78a32223e4a7dbc7b80b305226a2d2022-12-21T22:20:26ZengFrontiers Media S.A.Frontiers in Neuroinformatics1662-51962018-03-011210.3389/fninf.2018.00013332241A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult BrainIgnacio Arganda-Carreras0Ignacio Arganda-Carreras1Ignacio Arganda-Carreras2Tudor Manoliu3Nicolas Mazuras4Florian Schulze5Juan E. Iglesias6Katja Bühler7Arnim Jenett8François Rouyer9Philippe Andrey10Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, Versailles, FranceIkerbasque, Basque Foundation for Science, Bilbao, SpainDonostia International Physics Center, Donostia-San Sebastian, SpainInstitut des Neurosciences Paris-Saclay, Université Paris Sud, CNRS, Université Paris-Saclay, Gif-sur-Yvette, FranceInstitut des Neurosciences Paris-Saclay, Université Paris Sud, CNRS, Université Paris-Saclay, Gif-sur-Yvette, FranceVRVis Zentrum für Virtual Reality und Visualisierung Forschungs-GmbH, Vienna, AustriaBasque Center on Cognition, Brain and Language, Donostia-San Sebastian, SpainVRVis Zentrum für Virtual Reality und Visualisierung Forschungs-GmbH, Vienna, AustriaTefor Core Facility, Institut des Neurosciences Paris-Saclay, Université Paris Sud, CNRS, Université Paris-Saclay, Gif-sur-Yvette, FranceInstitut des Neurosciences Paris-Saclay, Université Paris Sud, CNRS, Université Paris-Saclay, Gif-sur-Yvette, FranceInstitut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, Versailles, FranceImaging the expression patterns of reporter constructs is a powerful tool to dissect the neuronal circuits of perception and behavior in the adult brain of Drosophila, one of the major models for studying brain functions. To date, several Drosophila brain templates and digital atlases have been built to automatically analyze and compare collections of expression pattern images. However, there has been no systematic comparison of performances between alternative atlasing strategies and registration algorithms. Here, we objectively evaluated the performance of different strategies for building adult Drosophila brain templates and atlases. In addition, we used state-of-the-art registration algorithms to generate a new group-wise inter-sex atlas. Our results highlight the benefit of statistical atlases over individual ones and show that the newly proposed inter-sex atlas outperformed existing solutions for automated registration and annotation of expression patterns. Over 3,000 images from the Janelia Farm FlyLight collection were registered using the proposed strategy. These registered expression patterns can be searched and compared with a new version of the BrainBaseWeb system and BrainGazer software. We illustrate the validity of our methodology and brain atlas with registration-based predictions of expression patterns in a subset of clock neurons. The described registration framework should benefit to brain studies in Drosophila and other insect species.http://journal.frontiersin.org/article/10.3389/fninf.2018.00013/fullDrosophila adult brainanatomical atlasconfocal microscopybrain mappingaverage brain templatediffeomorphic image registration |
| spellingShingle | Ignacio Arganda-Carreras Ignacio Arganda-Carreras Ignacio Arganda-Carreras Tudor Manoliu Nicolas Mazuras Florian Schulze Juan E. Iglesias Katja Bühler Arnim Jenett François Rouyer Philippe Andrey A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain Frontiers in Neuroinformatics Drosophila adult brain anatomical atlas confocal microscopy brain mapping average brain template diffeomorphic image registration |
| title | A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain |
| title_full | A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain |
| title_fullStr | A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain |
| title_full_unstemmed | A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain |
| title_short | A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain |
| title_sort | statistically representative atlas for mapping neuronal circuits in the drosophila adult brain |
| topic | Drosophila adult brain anatomical atlas confocal microscopy brain mapping average brain template diffeomorphic image registration |
| url | http://journal.frontiersin.org/article/10.3389/fninf.2018.00013/full |
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