Identification and Expression Profiling of <i>TGA</i> Transcription Factor Genes in Sugarcane Reveals the Roles in Response to <i>Sporisorium scitamineum</i> Infection
TGA transcription factor (TF) family genes play a major role in the regulation of plant growth and development as well as in the defense against pathogen attack. Little is known about the <i>TGA</i> family genes and their functions in sugarcane. Here, a total of 16 <i>TGA</i>...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/12/10/1644 |
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| author | Zhengying Luo Xin Hu Zhuandi Wu Xinlong Liu Caiwen Wu Qianchun Zeng |
| author_facet | Zhengying Luo Xin Hu Zhuandi Wu Xinlong Liu Caiwen Wu Qianchun Zeng |
| author_sort | Zhengying Luo |
| collection | DOAJ |
| description | TGA transcription factor (TF) family genes play a major role in the regulation of plant growth and development as well as in the defense against pathogen attack. Little is known about the <i>TGA</i> family genes and their functions in sugarcane. Here, a total of 16 <i>TGA</i> members were identified in the sugarcane genome by bioinformatic approaches. All members exhibited similar conserved motifs and contained a bZIP domain and a DOG1 domain, except for <i>ShTGA15/16</i>. Phylogenetic analysis demonstrated that 16 <i>ShTGA</i> family genes could be divided into eight clades, and evolved differently from <i>Arabidopsis</i> <i>TGAs</i>. All <i>ShTGA</i> family genes suffered a purifying selection during evolution. A wide range of cis-regulatory elements were found in the promoter of <i>ShTGA</i> genes including hormone regulatory elements, adversity response elements, light responsive elements, and growth and development regulatory elements. Most <i>ShTGA</i> expressions were increased in bud growth and developmental processes except for <i>ShTGA10</i><i>/11</i>. It is worth noting that the expression of <i>ShTGA13</i> was decreased after sugarcane was infected with <i>Sporisorium scitamineum</i>, and it was highly expressed in the resistant variety compared to the susceptible variety. Adding IAA, GA<sub>3</sub> and SA restored the expression of <i>ShTGA13</i>, suggesting an association with plant hormone regulatory pathways. Our study provides a framework for further functional studies of important <i>ShTGA</i> genes in development and stress response, and uncovered a previously unrecognized role of <i>ShTGA13</i> in regulating resistance against <i>S. scitamineum</i>. |
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| language | English |
| last_indexed | 2024-03-09T20:55:48Z |
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| series | Agriculture |
| spelling | doaj.art-c1a95837951240b4bce48a562a730f7d2023-11-23T22:21:45ZengMDPI AGAgriculture2077-04722022-10-011210164410.3390/agriculture12101644Identification and Expression Profiling of <i>TGA</i> Transcription Factor Genes in Sugarcane Reveals the Roles in Response to <i>Sporisorium scitamineum</i> InfectionZhengying Luo0Xin Hu1Zhuandi Wu2Xinlong Liu3Caiwen Wu4Qianchun Zeng5College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, ChinaYunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Kaiyuan 661699, ChinaYunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Kaiyuan 661699, ChinaYunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Kaiyuan 661699, ChinaYunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Kaiyuan 661699, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, ChinaTGA transcription factor (TF) family genes play a major role in the regulation of plant growth and development as well as in the defense against pathogen attack. Little is known about the <i>TGA</i> family genes and their functions in sugarcane. Here, a total of 16 <i>TGA</i> members were identified in the sugarcane genome by bioinformatic approaches. All members exhibited similar conserved motifs and contained a bZIP domain and a DOG1 domain, except for <i>ShTGA15/16</i>. Phylogenetic analysis demonstrated that 16 <i>ShTGA</i> family genes could be divided into eight clades, and evolved differently from <i>Arabidopsis</i> <i>TGAs</i>. All <i>ShTGA</i> family genes suffered a purifying selection during evolution. A wide range of cis-regulatory elements were found in the promoter of <i>ShTGA</i> genes including hormone regulatory elements, adversity response elements, light responsive elements, and growth and development regulatory elements. Most <i>ShTGA</i> expressions were increased in bud growth and developmental processes except for <i>ShTGA10</i><i>/11</i>. It is worth noting that the expression of <i>ShTGA13</i> was decreased after sugarcane was infected with <i>Sporisorium scitamineum</i>, and it was highly expressed in the resistant variety compared to the susceptible variety. Adding IAA, GA<sub>3</sub> and SA restored the expression of <i>ShTGA13</i>, suggesting an association with plant hormone regulatory pathways. Our study provides a framework for further functional studies of important <i>ShTGA</i> genes in development and stress response, and uncovered a previously unrecognized role of <i>ShTGA13</i> in regulating resistance against <i>S. scitamineum</i>.https://www.mdpi.com/2077-0472/12/10/1644sugarcaneTGA transcription factorgene expressionevolutionary analysis<i>Sporisorium scitamineum</i> |
| spellingShingle | Zhengying Luo Xin Hu Zhuandi Wu Xinlong Liu Caiwen Wu Qianchun Zeng Identification and Expression Profiling of <i>TGA</i> Transcription Factor Genes in Sugarcane Reveals the Roles in Response to <i>Sporisorium scitamineum</i> Infection Agriculture sugarcane TGA transcription factor gene expression evolutionary analysis <i>Sporisorium scitamineum</i> |
| title | Identification and Expression Profiling of <i>TGA</i> Transcription Factor Genes in Sugarcane Reveals the Roles in Response to <i>Sporisorium scitamineum</i> Infection |
| title_full | Identification and Expression Profiling of <i>TGA</i> Transcription Factor Genes in Sugarcane Reveals the Roles in Response to <i>Sporisorium scitamineum</i> Infection |
| title_fullStr | Identification and Expression Profiling of <i>TGA</i> Transcription Factor Genes in Sugarcane Reveals the Roles in Response to <i>Sporisorium scitamineum</i> Infection |
| title_full_unstemmed | Identification and Expression Profiling of <i>TGA</i> Transcription Factor Genes in Sugarcane Reveals the Roles in Response to <i>Sporisorium scitamineum</i> Infection |
| title_short | Identification and Expression Profiling of <i>TGA</i> Transcription Factor Genes in Sugarcane Reveals the Roles in Response to <i>Sporisorium scitamineum</i> Infection |
| title_sort | identification and expression profiling of i tga i transcription factor genes in sugarcane reveals the roles in response to i sporisorium scitamineum i infection |
| topic | sugarcane TGA transcription factor gene expression evolutionary analysis <i>Sporisorium scitamineum</i> |
| url | https://www.mdpi.com/2077-0472/12/10/1644 |
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