Reelin Alleviates Mesenchymal Stem Cell Senescence and Reduces Pathological α-Synuclein Expression in an In Vitro Model of Parkinson’s Disease
Parkinson’s disease (PD) is one of the most common neurodegenerative diseases. The mechanisms underlying PD remain to be fully elucidated, and research into treatments for this condition is ongoing. Recent advances in genetic research have shed light on the mechanisms underlying PD. In this study, w...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/7/1066 |
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| author | Eunju Cho Joonsang Park Kyungri Kim Min-Gi Kim Sung-Rae Cho |
| author_facet | Eunju Cho Joonsang Park Kyungri Kim Min-Gi Kim Sung-Rae Cho |
| author_sort | Eunju Cho |
| collection | DOAJ |
| description | Parkinson’s disease (PD) is one of the most common neurodegenerative diseases. The mechanisms underlying PD remain to be fully elucidated, and research into treatments for this condition is ongoing. Recent advances in genetic research have shed light on the mechanisms underlying PD. In this study, we used PD and control mesenchymal stem cells (MSCs) obtained from adipose tissues to confirm the differences between groups at the cellular and molecular levels. The results revealed that in PD MSCs, cell viability was clearly lower, and the rate of cell senescence was higher compared to the controls. Next, to compare the gene expression in PD and control cells, transcriptome analysis was performed. Genes in pathways, including extracellular matrix (ECM) receptor interaction, P53 signaling, and focal adhesion, were down-regulated in PD. Among genes related to ECM receptor interaction, <i>RELN</i> gene expression was markedly decreased in PD cells; however, after being treated with recombinant Reelin protein, a significant increase in cell viability and a decrease in α-Synuclein aggregation and cell senescence were observed. In conclusion, Reelin affects PD by positively influencing the cell characteristics. Our findings will facilitate research into new treatments for PD. |
| first_indexed | 2024-03-10T09:38:35Z |
| format | Article |
| id | doaj.art-c1a9693b1ca74e899cca2d9360576972 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T09:38:35Z |
| publishDate | 2021-07-01 |
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| series | Genes |
| spelling | doaj.art-c1a9693b1ca74e899cca2d93605769722023-11-22T03:51:06ZengMDPI AGGenes2073-44252021-07-01127106610.3390/genes12071066Reelin Alleviates Mesenchymal Stem Cell Senescence and Reduces Pathological α-Synuclein Expression in an In Vitro Model of Parkinson’s DiseaseEunju Cho0Joonsang Park1Kyungri Kim2Min-Gi Kim3Sung-Rae Cho4Department and Research Institute of Rehabilitation Medicine, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment and Research Institute of Rehabilitation Medicine, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment and Research Institute of Rehabilitation Medicine, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment and Research Institute of Rehabilitation Medicine, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment and Research Institute of Rehabilitation Medicine, Yonsei University College of Medicine, Seoul 03722, KoreaParkinson’s disease (PD) is one of the most common neurodegenerative diseases. The mechanisms underlying PD remain to be fully elucidated, and research into treatments for this condition is ongoing. Recent advances in genetic research have shed light on the mechanisms underlying PD. In this study, we used PD and control mesenchymal stem cells (MSCs) obtained from adipose tissues to confirm the differences between groups at the cellular and molecular levels. The results revealed that in PD MSCs, cell viability was clearly lower, and the rate of cell senescence was higher compared to the controls. Next, to compare the gene expression in PD and control cells, transcriptome analysis was performed. Genes in pathways, including extracellular matrix (ECM) receptor interaction, P53 signaling, and focal adhesion, were down-regulated in PD. Among genes related to ECM receptor interaction, <i>RELN</i> gene expression was markedly decreased in PD cells; however, after being treated with recombinant Reelin protein, a significant increase in cell viability and a decrease in α-Synuclein aggregation and cell senescence were observed. In conclusion, Reelin affects PD by positively influencing the cell characteristics. Our findings will facilitate research into new treatments for PD.https://www.mdpi.com/2073-4425/12/7/1066parkinson’s diseasetranscriptome analysisextracellular matrix-receptor related genesReelin |
| spellingShingle | Eunju Cho Joonsang Park Kyungri Kim Min-Gi Kim Sung-Rae Cho Reelin Alleviates Mesenchymal Stem Cell Senescence and Reduces Pathological α-Synuclein Expression in an In Vitro Model of Parkinson’s Disease Genes parkinson’s disease transcriptome analysis extracellular matrix-receptor related genes Reelin |
| title | Reelin Alleviates Mesenchymal Stem Cell Senescence and Reduces Pathological α-Synuclein Expression in an In Vitro Model of Parkinson’s Disease |
| title_full | Reelin Alleviates Mesenchymal Stem Cell Senescence and Reduces Pathological α-Synuclein Expression in an In Vitro Model of Parkinson’s Disease |
| title_fullStr | Reelin Alleviates Mesenchymal Stem Cell Senescence and Reduces Pathological α-Synuclein Expression in an In Vitro Model of Parkinson’s Disease |
| title_full_unstemmed | Reelin Alleviates Mesenchymal Stem Cell Senescence and Reduces Pathological α-Synuclein Expression in an In Vitro Model of Parkinson’s Disease |
| title_short | Reelin Alleviates Mesenchymal Stem Cell Senescence and Reduces Pathological α-Synuclein Expression in an In Vitro Model of Parkinson’s Disease |
| title_sort | reelin alleviates mesenchymal stem cell senescence and reduces pathological α synuclein expression in an in vitro model of parkinson s disease |
| topic | parkinson’s disease transcriptome analysis extracellular matrix-receptor related genes Reelin |
| url | https://www.mdpi.com/2073-4425/12/7/1066 |
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