Osseointegration Potential Assessment of Bone Graft Materials Loaded with Mesenchymal Stem Cells in Peri-Implant Bone Defects
Many studies have been exploring the use of bone graft materials (BGMs) and mesenchymal stem cells in bone defect reconstruction. However, the regeneration potential of Algipore (highly purified hydroxyapatite) and Biphasic (hydroxyapatite/beta-tricalcium phosphate) BGMs combined with bone marrow–de...
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MDPI AG
2024-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/2/862 |
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| author | Kuo-Fang Tseng Shiau-Ting Shiu Chia-Yi Hung Ya-Hui Chan Tze-Jian Chee Pai-Chun Huang Pin-Chuang Lai Sheng-Wei Feng |
| author_facet | Kuo-Fang Tseng Shiau-Ting Shiu Chia-Yi Hung Ya-Hui Chan Tze-Jian Chee Pai-Chun Huang Pin-Chuang Lai Sheng-Wei Feng |
| author_sort | Kuo-Fang Tseng |
| collection | DOAJ |
| description | Many studies have been exploring the use of bone graft materials (BGMs) and mesenchymal stem cells in bone defect reconstruction. However, the regeneration potential of Algipore (highly purified hydroxyapatite) and Biphasic (hydroxyapatite/beta-tricalcium phosphate) BGMs combined with bone marrow–derived mesenchymal stem cells (BMSCs) remains unclear. Therefore, we evaluated their osseointegration capacities in reconstructing peri-implant bone defects. The cellular characteristics of BMSCs and the material properties of Algipore and Biphasic were assessed in vitro. Four experimental groups—Algipore, Biphasic, Algipore+BMSCs, and Biphasic+BMSCs—were designed in a rabbit tibia peri-implant defect model. Implant stability parameters were measured. After 4 and 8 weeks of healing, all samples were evaluated using micro-CT, histological, and histomorphometric analysis. In the energy-dispersive X-ray spectroscopy experiment, the Ca/P ratio was higher for Algipore (1.67) than for Biphasic (1.44). The ISQ values continuously increased, and the PTV values gradually decreased for all groups during the healing period. Both Algipore and Biphasic BGM promoted new bone regeneration. Higher implant stability and bone volume density were observed when Algipore and Biphasic BGMs were combined with BMSCs. Biphasic BGM exhibited a faster degradation rate than Algipore BGM. Notably, after eight weeks of healing, Algipore with BSMCs showed more bone–implant contact than Biphasic alone (<i>p</i> < 0.05). Both Algipore and Biphasic are efficient in reconstructing peri-implant bone defects. In addition, Algipore BGM incorporation with BSMCs displayed the best performance in enhancing implant stability and osseointegration potential. |
| first_indexed | 2024-03-08T09:54:09Z |
| format | Article |
| id | doaj.art-c1aa74ed14f949c19223273d56f28cd5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T09:54:09Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c1aa74ed14f949c19223273d56f28cd52024-01-29T13:55:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-01-0125286210.3390/ijms25020862Osseointegration Potential Assessment of Bone Graft Materials Loaded with Mesenchymal Stem Cells in Peri-Implant Bone DefectsKuo-Fang Tseng0Shiau-Ting Shiu1Chia-Yi Hung2Ya-Hui Chan3Tze-Jian Chee4Pai-Chun Huang5Pin-Chuang Lai6Sheng-Wei Feng7School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei City 110301, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei City 110301, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei City 110301, TaiwanSchool of Oral Hygiene, College of Oral Medicine, Taipei Medical University, Taipei City 110301, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei City 110301, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei City 110301, TaiwanDepartment of Periodontics, School of Dentistry, University of Missouri-Kansas City, Kansas City, MO 64108, USASchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei City 110301, TaiwanMany studies have been exploring the use of bone graft materials (BGMs) and mesenchymal stem cells in bone defect reconstruction. However, the regeneration potential of Algipore (highly purified hydroxyapatite) and Biphasic (hydroxyapatite/beta-tricalcium phosphate) BGMs combined with bone marrow–derived mesenchymal stem cells (BMSCs) remains unclear. Therefore, we evaluated their osseointegration capacities in reconstructing peri-implant bone defects. The cellular characteristics of BMSCs and the material properties of Algipore and Biphasic were assessed in vitro. Four experimental groups—Algipore, Biphasic, Algipore+BMSCs, and Biphasic+BMSCs—were designed in a rabbit tibia peri-implant defect model. Implant stability parameters were measured. After 4 and 8 weeks of healing, all samples were evaluated using micro-CT, histological, and histomorphometric analysis. In the energy-dispersive X-ray spectroscopy experiment, the Ca/P ratio was higher for Algipore (1.67) than for Biphasic (1.44). The ISQ values continuously increased, and the PTV values gradually decreased for all groups during the healing period. Both Algipore and Biphasic BGM promoted new bone regeneration. Higher implant stability and bone volume density were observed when Algipore and Biphasic BGMs were combined with BMSCs. Biphasic BGM exhibited a faster degradation rate than Algipore BGM. Notably, after eight weeks of healing, Algipore with BSMCs showed more bone–implant contact than Biphasic alone (<i>p</i> < 0.05). Both Algipore and Biphasic are efficient in reconstructing peri-implant bone defects. In addition, Algipore BGM incorporation with BSMCs displayed the best performance in enhancing implant stability and osseointegration potential.https://www.mdpi.com/1422-0067/25/2/862implant stabilityosseointegrationperi-implant defectbone graft materialsmesenchymal stem cells |
| spellingShingle | Kuo-Fang Tseng Shiau-Ting Shiu Chia-Yi Hung Ya-Hui Chan Tze-Jian Chee Pai-Chun Huang Pin-Chuang Lai Sheng-Wei Feng Osseointegration Potential Assessment of Bone Graft Materials Loaded with Mesenchymal Stem Cells in Peri-Implant Bone Defects International Journal of Molecular Sciences implant stability osseointegration peri-implant defect bone graft materials mesenchymal stem cells |
| title | Osseointegration Potential Assessment of Bone Graft Materials Loaded with Mesenchymal Stem Cells in Peri-Implant Bone Defects |
| title_full | Osseointegration Potential Assessment of Bone Graft Materials Loaded with Mesenchymal Stem Cells in Peri-Implant Bone Defects |
| title_fullStr | Osseointegration Potential Assessment of Bone Graft Materials Loaded with Mesenchymal Stem Cells in Peri-Implant Bone Defects |
| title_full_unstemmed | Osseointegration Potential Assessment of Bone Graft Materials Loaded with Mesenchymal Stem Cells in Peri-Implant Bone Defects |
| title_short | Osseointegration Potential Assessment of Bone Graft Materials Loaded with Mesenchymal Stem Cells in Peri-Implant Bone Defects |
| title_sort | osseointegration potential assessment of bone graft materials loaded with mesenchymal stem cells in peri implant bone defects |
| topic | implant stability osseointegration peri-implant defect bone graft materials mesenchymal stem cells |
| url | https://www.mdpi.com/1422-0067/25/2/862 |
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