A low-carbon high inulin diet improves intestinal mucosal barrier function and immunity against infectious diseases in goats
IntroductionAbrupt weaning is a major stressful event, contributing to intestinal abnormalities and immune system dysfunction in weaned kids. Inulin is a prebiotic fiber with many positive functions, including promoting intestinal fermentation and enhancing host immunity in monogastric animals. Howe...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.1098651/full |
| _version_ | 1797955993813909504 |
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| author | Chunmei Yuan Chunmei Yuan Shuiping Wang Kefyalew Gebeyew Xin Yang Xin Yang Shaoxun Tang Chuanshe Zhou Chuanshe Zhou Nazir Ahmad Khan Nazir Ahmad Khan Zhiliang Tan Yong Liu |
| author_facet | Chunmei Yuan Chunmei Yuan Shuiping Wang Kefyalew Gebeyew Xin Yang Xin Yang Shaoxun Tang Chuanshe Zhou Chuanshe Zhou Nazir Ahmad Khan Nazir Ahmad Khan Zhiliang Tan Yong Liu |
| author_sort | Chunmei Yuan |
| collection | DOAJ |
| description | IntroductionAbrupt weaning is a major stressful event, contributing to intestinal abnormalities and immune system dysfunction in weaned kids. Inulin is a prebiotic fiber with many positive functions, including promoting intestinal fermentation and enhancing host immunity in monogastric animals. However, the effects of a high-inulin, energy-rich diet on ruminal fermentation characteristics, methane emission, growth performance, and immune systems of weaned kids have not been investigated.MethodsA fully automated in vitro fermentation system was used to investigate ruminal fermentation characteristics and methane emission of a mixed substrate of inulin and fat powder (1.31: 1) in comparison with maize grain-based starter concentrate. During a 1-week adaptation and 4-week trial phase, 18 weaned kids (8.97 ± 0.19 kg) were randomly assigned to two groups, one with a conventional diet (83% maize grain; CON) and the other with a low-carbon, high-inulin diet (41.5% maize grain, 14.4% fat powder, 18.9% inulin; INU).ResultsIn the in vitro rumen fermentation experiment, the total gas production was not different (p > 0.05); however, a lower (p < 0.05) methane production was observed for INU as compared to CON. The average daily gain and the ratio of feed intake and growth performance of kids fed with INU were higher (p < 0.05) than those fed with CON. Serum concentrations of alanine transaminase (ALT) and lactate dehydrogenase (LDH) were lower (p < 0.05), whereas the concentration of high-density lipoprotein (HDL) and cholesterol (CHOL) were higher (p < 0.05) in kids fed with the INU diet as compared CON. Dietary inulin significantly increased (p < 0.05) the secretion of immunoglobulins (IgA, IgG, and IgM) and inflammatory cytokines (IFN-γ and IL-10) in ileum tissue. Although no differences (p > 0.05) were observed in mRNA expression of tight junction markers, the INU diet tended to increase (p = 0.09) gene expression of ribosomal protein S6 kinase beta-1 (P70S6K) in the mammalian target of rapamycin (mTOR) pathway of longissimus dorsi muscle.ConclusionOur findings highlighted that a low-carbon high-inulin energy-rich diet could be used as a promising strategy to improve gut immunity and growth performance of weaned kids under abrupt weaning stress and reduce methane production. |
| first_indexed | 2024-04-10T23:43:00Z |
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| id | doaj.art-c1abba4a1592400a8e370d103fa8e9c8 |
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| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-10T23:43:00Z |
| publishDate | 2023-01-01 |
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| series | Frontiers in Veterinary Science |
| spelling | doaj.art-c1abba4a1592400a8e370d103fa8e9c82023-01-11T06:26:50ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-01-01910.3389/fvets.2022.10986511098651A low-carbon high inulin diet improves intestinal mucosal barrier function and immunity against infectious diseases in goatsChunmei Yuan0Chunmei Yuan1Shuiping Wang2Kefyalew Gebeyew3Xin Yang4Xin Yang5Shaoxun Tang6Chuanshe Zhou7Chuanshe Zhou8Nazir Ahmad Khan9Nazir Ahmad Khan10Zhiliang Tan11Yong Liu12Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan, ChinaUniversity of the Chinese Academy of Sciences, Beijing, ChinaChongqing Key Laboratory of Herbivore Science, College of Animal Science and Technology, Southwest University, Chongqing, ChinaKey Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan, ChinaKey Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan, ChinaChongqing Key Laboratory of Herbivore Science, College of Animal Science and Technology, Southwest University, Chongqing, ChinaKey Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan, ChinaKey Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan, ChinaUniversity of the Chinese Academy of Sciences, Beijing, ChinaKey Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan, ChinaDepartment of Animal Nutrition, The University of Agriculture, Peshawar, KP, PakistanKey Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan, ChinaKey Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan, ChinaIntroductionAbrupt weaning is a major stressful event, contributing to intestinal abnormalities and immune system dysfunction in weaned kids. Inulin is a prebiotic fiber with many positive functions, including promoting intestinal fermentation and enhancing host immunity in monogastric animals. However, the effects of a high-inulin, energy-rich diet on ruminal fermentation characteristics, methane emission, growth performance, and immune systems of weaned kids have not been investigated.MethodsA fully automated in vitro fermentation system was used to investigate ruminal fermentation characteristics and methane emission of a mixed substrate of inulin and fat powder (1.31: 1) in comparison with maize grain-based starter concentrate. During a 1-week adaptation and 4-week trial phase, 18 weaned kids (8.97 ± 0.19 kg) were randomly assigned to two groups, one with a conventional diet (83% maize grain; CON) and the other with a low-carbon, high-inulin diet (41.5% maize grain, 14.4% fat powder, 18.9% inulin; INU).ResultsIn the in vitro rumen fermentation experiment, the total gas production was not different (p > 0.05); however, a lower (p < 0.05) methane production was observed for INU as compared to CON. The average daily gain and the ratio of feed intake and growth performance of kids fed with INU were higher (p < 0.05) than those fed with CON. Serum concentrations of alanine transaminase (ALT) and lactate dehydrogenase (LDH) were lower (p < 0.05), whereas the concentration of high-density lipoprotein (HDL) and cholesterol (CHOL) were higher (p < 0.05) in kids fed with the INU diet as compared CON. Dietary inulin significantly increased (p < 0.05) the secretion of immunoglobulins (IgA, IgG, and IgM) and inflammatory cytokines (IFN-γ and IL-10) in ileum tissue. Although no differences (p > 0.05) were observed in mRNA expression of tight junction markers, the INU diet tended to increase (p = 0.09) gene expression of ribosomal protein S6 kinase beta-1 (P70S6K) in the mammalian target of rapamycin (mTOR) pathway of longissimus dorsi muscle.ConclusionOur findings highlighted that a low-carbon high-inulin energy-rich diet could be used as a promising strategy to improve gut immunity and growth performance of weaned kids under abrupt weaning stress and reduce methane production.https://www.frontiersin.org/articles/10.3389/fvets.2022.1098651/fullinulinintestinal immunitygrowth performancemTOR pathwayweaned goats |
| spellingShingle | Chunmei Yuan Chunmei Yuan Shuiping Wang Kefyalew Gebeyew Xin Yang Xin Yang Shaoxun Tang Chuanshe Zhou Chuanshe Zhou Nazir Ahmad Khan Nazir Ahmad Khan Zhiliang Tan Yong Liu A low-carbon high inulin diet improves intestinal mucosal barrier function and immunity against infectious diseases in goats Frontiers in Veterinary Science inulin intestinal immunity growth performance mTOR pathway weaned goats |
| title | A low-carbon high inulin diet improves intestinal mucosal barrier function and immunity against infectious diseases in goats |
| title_full | A low-carbon high inulin diet improves intestinal mucosal barrier function and immunity against infectious diseases in goats |
| title_fullStr | A low-carbon high inulin diet improves intestinal mucosal barrier function and immunity against infectious diseases in goats |
| title_full_unstemmed | A low-carbon high inulin diet improves intestinal mucosal barrier function and immunity against infectious diseases in goats |
| title_short | A low-carbon high inulin diet improves intestinal mucosal barrier function and immunity against infectious diseases in goats |
| title_sort | low carbon high inulin diet improves intestinal mucosal barrier function and immunity against infectious diseases in goats |
| topic | inulin intestinal immunity growth performance mTOR pathway weaned goats |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.1098651/full |
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