Microbiological assessment of the effectiveness of standard therapy in atopic dermatitis
Background. Atopic dermatitis is an inflammatory skin disease characterized by recurrent lesions and intense pruritus. Nowadays there is a stepwise approach to the treatment of atopic dermatitis, which is defined by disease intensity and complications such as secondary skin infections. However, the...
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| Format: | Article |
| Language: | English |
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State Scientific Center of Dermatovenereology and Cosmetology
2023-07-01
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| Series: | Vestnik Dermatologii i Venerologii |
| Subjects: | |
| Online Access: | https://vestnikdv.ru/jour/article/viewFile/1364/pdf |
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| author | Olga Yu. Olisova Oxana A. Svitich Alexander V. Poddubikov Nune O. Vartanova Mariia B. Potapova |
| author_facet | Olga Yu. Olisova Oxana A. Svitich Alexander V. Poddubikov Nune O. Vartanova Mariia B. Potapova |
| author_sort | Olga Yu. Olisova |
| collection | DOAJ |
| description | Background. Atopic dermatitis is an inflammatory skin disease characterized by recurrent lesions and intense pruritus. Nowadays there is a stepwise approach to the treatment of atopic dermatitis, which is defined by disease intensity and complications such as secondary skin infections. However, the current management of atopic dermatitis may not always lead to the expected outcome due to not only immune dysregulation of both adaptive and innate immunity but also imbalance of the skin microbiome.
Aims. The aim of the study was to evaluate changes in the composition of the skin microbiome in both lesional and non-lesional skin in patients with atopic dermatitis during standard treatment.
Materials and methods. Twenty patients with atopic dermatitis and twenty six healthy controls over 18 years old were included into the study. All microbiome samples were obtained from lesional and non-lesional skin sites of atopic dermatitis patients before and after therapy. Whereas samples from healthy controls were taken once from a flexor surface of the elbow. Species identification of clinical isolates were identified using MALDI Biotyper Sirius (Bruker Daltonics).
Results. At baseline, the prevalence of S. aureus colonization among patients with atopic dermatitis was 34.20% in lesional skin and 32.50% in non-lesional skin. After treatment, there was a significant decrease in the prevalence of S. aureus carriage in both lesional and non-lesional skin areas (р 0.05). However, no significant difference was observed in the proportion of all other staphylococci (р 0.1). Interestingly, S. aureus was not found in healthy controls.
Conclusions. The results of the study demonstrated the effectiveness of standard therapy for managing patients with atopic dermatitis as it had a positive impact on the skin microbial community and showed a decrease in S. aureus proportion after the treatment. |
| first_indexed | 2024-03-12T20:33:36Z |
| format | Article |
| id | doaj.art-c1ac96f0cd0840c19587624cbd1edc53 |
| institution | Directory Open Access Journal |
| issn | 0042-4609 2313-6294 |
| language | English |
| last_indexed | 2024-03-12T20:33:36Z |
| publishDate | 2023-07-01 |
| publisher | State Scientific Center of Dermatovenereology and Cosmetology |
| record_format | Article |
| series | Vestnik Dermatologii i Venerologii |
| spelling | doaj.art-c1ac96f0cd0840c19587624cbd1edc532023-08-01T18:03:07ZengState Scientific Center of Dermatovenereology and CosmetologyVestnik Dermatologii i Venerologii0042-46092313-62942023-07-01993445210.25208/vdv13641224Microbiological assessment of the effectiveness of standard therapy in atopic dermatitisOlga Yu. Olisova0https://orcid.org/0000-0003-2482-1754Oxana A. Svitich1https://orcid.org/0000-0003-1757-8389Alexander V. Poddubikov2https://orcid.org/0000-0001-8962-4765Nune O. Vartanova3https://orcid.org/0000-0002-6372-9910Mariia B. Potapova4https://orcid.org/0000-0001-9647-1322Sechenov UniversitySechenov UniversityI. I. Mechnikov Research Institute of Vaccines and SeraI. I. Mechnikov Research Institute of Vaccines and SeraSechenov UniversityBackground. Atopic dermatitis is an inflammatory skin disease characterized by recurrent lesions and intense pruritus. Nowadays there is a stepwise approach to the treatment of atopic dermatitis, which is defined by disease intensity and complications such as secondary skin infections. However, the current management of atopic dermatitis may not always lead to the expected outcome due to not only immune dysregulation of both adaptive and innate immunity but also imbalance of the skin microbiome. Aims. The aim of the study was to evaluate changes in the composition of the skin microbiome in both lesional and non-lesional skin in patients with atopic dermatitis during standard treatment. Materials and methods. Twenty patients with atopic dermatitis and twenty six healthy controls over 18 years old were included into the study. All microbiome samples were obtained from lesional and non-lesional skin sites of atopic dermatitis patients before and after therapy. Whereas samples from healthy controls were taken once from a flexor surface of the elbow. Species identification of clinical isolates were identified using MALDI Biotyper Sirius (Bruker Daltonics). Results. At baseline, the prevalence of S. aureus colonization among patients with atopic dermatitis was 34.20% in lesional skin and 32.50% in non-lesional skin. After treatment, there was a significant decrease in the prevalence of S. aureus carriage in both lesional and non-lesional skin areas (р 0.05). However, no significant difference was observed in the proportion of all other staphylococci (р 0.1). Interestingly, S. aureus was not found in healthy controls. Conclusions. The results of the study demonstrated the effectiveness of standard therapy for managing patients with atopic dermatitis as it had a positive impact on the skin microbial community and showed a decrease in S. aureus proportion after the treatment.https://vestnikdv.ru/jour/article/viewFile/1364/pdfmicrobiomeatopic dermatitisstaphylococcus aureus |
| spellingShingle | Olga Yu. Olisova Oxana A. Svitich Alexander V. Poddubikov Nune O. Vartanova Mariia B. Potapova Microbiological assessment of the effectiveness of standard therapy in atopic dermatitis Vestnik Dermatologii i Venerologii microbiome atopic dermatitis staphylococcus aureus |
| title | Microbiological assessment of the effectiveness of standard therapy in atopic dermatitis |
| title_full | Microbiological assessment of the effectiveness of standard therapy in atopic dermatitis |
| title_fullStr | Microbiological assessment of the effectiveness of standard therapy in atopic dermatitis |
| title_full_unstemmed | Microbiological assessment of the effectiveness of standard therapy in atopic dermatitis |
| title_short | Microbiological assessment of the effectiveness of standard therapy in atopic dermatitis |
| title_sort | microbiological assessment of the effectiveness of standard therapy in atopic dermatitis |
| topic | microbiome atopic dermatitis staphylococcus aureus |
| url | https://vestnikdv.ru/jour/article/viewFile/1364/pdf |
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