Orexin A and Orexin Receptor 1 axonal traffic in dorsal roots at the CNS/PNS interface
Hypothalamic orexin/hypocretin neurons send long axonal projections through the dorsal spinal cord in lamina I-II of the dorsal horn at the interface with the peripheral nervous system (PNS). We show that in the dorsal horn OXA fibers colocalize with substance P (SP) positive afferents of dorsal roo...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-02-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnins.2014.00020/full |
| _version_ | 1818657567011766272 |
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| author | Damien eColas Damien eColas Annalisa eManca Jean-Dominique eDelcroix Philippe eMourrain Philippe eMourrain |
| author_facet | Damien eColas Damien eColas Annalisa eManca Jean-Dominique eDelcroix Philippe eMourrain Philippe eMourrain |
| author_sort | Damien eColas |
| collection | DOAJ |
| description | Hypothalamic orexin/hypocretin neurons send long axonal projections through the dorsal spinal cord in lamina I-II of the dorsal horn at the interface with the peripheral nervous system (PNS). We show that in the dorsal horn OXA fibers colocalize with substance P (SP) positive afferents of dorsal root ganglia (DRG) neurons known to mediate sensory processing. Further, OR1 is expressed in p75NTR and SP positive DRG neurons, suggesting a potential signaling pathway between orexin and DRG neurons. Interestingly, DRG sensory neurons have a distinctive bifurcating axon where one branch innervates the periphery and the other one the spinal cord (pseudo-unipolar neurons), allowing for potential functional coupling of distinct targets. We observe that OR1 is transported selectively from DRG toward the spinal cord, while OXA is accumulated retrogradely toward the DRG. We hence report a rare situation of asymmetrical neuropeptide receptor distribution between axons projected by a single neuron. This molecular and cellular data are consistent with the role of OXA/OR1 in sensory processing, including DRG neuronal modulation, and support the potential existence of an OX/HCRT circuit between CNS and PNS. |
| first_indexed | 2024-12-17T03:43:31Z |
| format | Article |
| id | doaj.art-c1aee84489144927a9e6ed544672fcdc |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-12-17T03:43:31Z |
| publishDate | 2014-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-c1aee84489144927a9e6ed544672fcdc2022-12-21T22:04:56ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2014-02-01810.3389/fnins.2014.0002068166Orexin A and Orexin Receptor 1 axonal traffic in dorsal roots at the CNS/PNS interfaceDamien eColas0Damien eColas1Annalisa eManca2Jean-Dominique eDelcroix3Philippe eMourrain4Philippe eMourrain5Stanford UniversityEuropean Brain Research InstituteEuropean Brain Research InstituteEuropean Brain Research InstituteStanford UniversityINSERM 1024Hypothalamic orexin/hypocretin neurons send long axonal projections through the dorsal spinal cord in lamina I-II of the dorsal horn at the interface with the peripheral nervous system (PNS). We show that in the dorsal horn OXA fibers colocalize with substance P (SP) positive afferents of dorsal root ganglia (DRG) neurons known to mediate sensory processing. Further, OR1 is expressed in p75NTR and SP positive DRG neurons, suggesting a potential signaling pathway between orexin and DRG neurons. Interestingly, DRG sensory neurons have a distinctive bifurcating axon where one branch innervates the periphery and the other one the spinal cord (pseudo-unipolar neurons), allowing for potential functional coupling of distinct targets. We observe that OR1 is transported selectively from DRG toward the spinal cord, while OXA is accumulated retrogradely toward the DRG. We hence report a rare situation of asymmetrical neuropeptide receptor distribution between axons projected by a single neuron. This molecular and cellular data are consistent with the role of OXA/OR1 in sensory processing, including DRG neuronal modulation, and support the potential existence of an OX/HCRT circuit between CNS and PNS.http://journal.frontiersin.org/Journal/10.3389/fnins.2014.00020/fullAxonal TransportNociceptionSubstance Pdorsal root gangliahypocretinorexin |
| spellingShingle | Damien eColas Damien eColas Annalisa eManca Jean-Dominique eDelcroix Philippe eMourrain Philippe eMourrain Orexin A and Orexin Receptor 1 axonal traffic in dorsal roots at the CNS/PNS interface Frontiers in Neuroscience Axonal Transport Nociception Substance P dorsal root ganglia hypocretin orexin |
| title | Orexin A and Orexin Receptor 1 axonal traffic in dorsal roots at the CNS/PNS interface |
| title_full | Orexin A and Orexin Receptor 1 axonal traffic in dorsal roots at the CNS/PNS interface |
| title_fullStr | Orexin A and Orexin Receptor 1 axonal traffic in dorsal roots at the CNS/PNS interface |
| title_full_unstemmed | Orexin A and Orexin Receptor 1 axonal traffic in dorsal roots at the CNS/PNS interface |
| title_short | Orexin A and Orexin Receptor 1 axonal traffic in dorsal roots at the CNS/PNS interface |
| title_sort | orexin a and orexin receptor 1 axonal traffic in dorsal roots at the cns pns interface |
| topic | Axonal Transport Nociception Substance P dorsal root ganglia hypocretin orexin |
| url | http://journal.frontiersin.org/Journal/10.3389/fnins.2014.00020/full |
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