Identification of Volatile Components of Liverwort (<em>Porella cordaeana</em>) Extracts Using GC/MS-SPME and Their Antimicrobial Activity
Chemical constituents of liverwort (<em>Porella cordaeana</em>) extracts have been identified using solid-phase microextraction-gas chromatography mass spectrometry (SPME-GC/MS). The methanol, ethanol and ethyl acetate extracts were rich in terpenoids such as sesquiterpen...
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MDPI AG
2012-06-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/17/6/6982 |
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| author | Maria Elisabetta Guerzoni Danka Bukvicki Lucia Vannini Petar D. Marin Milan Veljic Davide Gottardi |
| author_facet | Maria Elisabetta Guerzoni Danka Bukvicki Lucia Vannini Petar D. Marin Milan Veljic Davide Gottardi |
| author_sort | Maria Elisabetta Guerzoni |
| collection | DOAJ |
| description | Chemical constituents of liverwort (<em>Porella cordaeana</em>) extracts have been identified using solid-phase microextraction-gas chromatography mass spectrometry (SPME-GC/MS). The methanol, ethanol and ethyl acetate extracts were rich in terpenoids such as sesquiterpene hydrocarbons (53.12%, 51.68%, 23.16%), and monoterpene hydrocarbons (22.83%, 18.90%, 23.36%), respectively. The dominant compounds in the extracts were β-phellandrene (15.54%, 13.66%, 12.10%) and β-caryophyllene (10.72%, 8.29%, 7.79%, respectively). The antimicrobial activity of the extracts was evaluated against eleven food microorganisms using the microdilution and disc diffusion methods. The minimum inhibitory concentration (MIC) varied from 0.50 to 2.00 mg/mL for yeast strains (<em>Saccharomyces cerevisiae 635</em>, <em>Zygosacharomyces bailii 45</em>, <em>Aerobasidium pullulans L6F</em>, <em>Pichia membranaefaciens OC 71</em>, <em>Pichia membranaefaciens OC 70</em>, <em>Pichia anomala</em> <em>CBS 5759</em>, <em>Pichia anomala DBVPG 3003</em> and<strong><em> </em></strong><em>Yarrowia lipolytica RO13</em>), and from 1.00 to 3.00 mg/mL for bacterial strains<em> </em>(<em>Salmonella</em> <em>enteritidis 155</em>, <em>Escherichia coli 555</em> and <em>Listeria monocytogenes 56Ly</em>). Methanol extract showed better activity in comparison with ethanol and ethyl acetate extracts. High percentages of monoterpene and sesquiterpene hydrocarbons could be responsible for the better antimicrobial activity. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-13T06:56:43Z |
| publishDate | 2012-06-01 |
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| series | Molecules |
| spelling | doaj.art-c1b0d60e560d40638c4e84564b1abfb32022-12-21T23:56:01ZengMDPI AGMolecules1420-30492012-06-011766982699510.3390/molecules17066982Identification of Volatile Components of Liverwort (<em>Porella cordaeana</em>) Extracts Using GC/MS-SPME and Their Antimicrobial ActivityMaria Elisabetta GuerzoniDanka BukvickiLucia VanniniPetar D. MarinMilan VeljicDavide GottardiChemical constituents of liverwort (<em>Porella cordaeana</em>) extracts have been identified using solid-phase microextraction-gas chromatography mass spectrometry (SPME-GC/MS). The methanol, ethanol and ethyl acetate extracts were rich in terpenoids such as sesquiterpene hydrocarbons (53.12%, 51.68%, 23.16%), and monoterpene hydrocarbons (22.83%, 18.90%, 23.36%), respectively. The dominant compounds in the extracts were β-phellandrene (15.54%, 13.66%, 12.10%) and β-caryophyllene (10.72%, 8.29%, 7.79%, respectively). The antimicrobial activity of the extracts was evaluated against eleven food microorganisms using the microdilution and disc diffusion methods. The minimum inhibitory concentration (MIC) varied from 0.50 to 2.00 mg/mL for yeast strains (<em>Saccharomyces cerevisiae 635</em>, <em>Zygosacharomyces bailii 45</em>, <em>Aerobasidium pullulans L6F</em>, <em>Pichia membranaefaciens OC 71</em>, <em>Pichia membranaefaciens OC 70</em>, <em>Pichia anomala</em> <em>CBS 5759</em>, <em>Pichia anomala DBVPG 3003</em> and<strong><em> </em></strong><em>Yarrowia lipolytica RO13</em>), and from 1.00 to 3.00 mg/mL for bacterial strains<em> </em>(<em>Salmonella</em> <em>enteritidis 155</em>, <em>Escherichia coli 555</em> and <em>Listeria monocytogenes 56Ly</em>). Methanol extract showed better activity in comparison with ethanol and ethyl acetate extracts. High percentages of monoterpene and sesquiterpene hydrocarbons could be responsible for the better antimicrobial activity.http://www.mdpi.com/1420-3049/17/6/6982liverwort<em>Porella cordaeana</em>GC/MS-SPMEantimicrobial activityfood microorganisms |
| spellingShingle | Maria Elisabetta Guerzoni Danka Bukvicki Lucia Vannini Petar D. Marin Milan Veljic Davide Gottardi Identification of Volatile Components of Liverwort (<em>Porella cordaeana</em>) Extracts Using GC/MS-SPME and Their Antimicrobial Activity Molecules liverwort <em>Porella cordaeana</em> GC/MS-SPME antimicrobial activity food microorganisms |
| title | Identification of Volatile Components of Liverwort (<em>Porella cordaeana</em>) Extracts Using GC/MS-SPME and Their Antimicrobial Activity |
| title_full | Identification of Volatile Components of Liverwort (<em>Porella cordaeana</em>) Extracts Using GC/MS-SPME and Their Antimicrobial Activity |
| title_fullStr | Identification of Volatile Components of Liverwort (<em>Porella cordaeana</em>) Extracts Using GC/MS-SPME and Their Antimicrobial Activity |
| title_full_unstemmed | Identification of Volatile Components of Liverwort (<em>Porella cordaeana</em>) Extracts Using GC/MS-SPME and Their Antimicrobial Activity |
| title_short | Identification of Volatile Components of Liverwort (<em>Porella cordaeana</em>) Extracts Using GC/MS-SPME and Their Antimicrobial Activity |
| title_sort | identification of volatile components of liverwort lt em gt porella cordaeana lt em gt extracts using gc ms spme and their antimicrobial activity |
| topic | liverwort <em>Porella cordaeana</em> GC/MS-SPME antimicrobial activity food microorganisms |
| url | http://www.mdpi.com/1420-3049/17/6/6982 |
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