The Indiscriminate Chemical Makeup of Secondary Metabolites Derived from Endophytes Harvested from <i>Aloe barbadensis</i> Miller in South Africa’s Limpopo Region
The efficacy of 23 bacterial isolates obtained from surface-sterilized stems and leaves of three medicinal plants (<i>Aloe barbadensis</i> Miller, <i>Artemisia afra</i>, and <i>Moringa oleifera</i>) was investigated in an endeavour to prevent the growth of <i&g...
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MDPI AG
2024-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/6/1297 |
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| author | Mpho Mamphoka Nchabeleng Thierry Youmbi Fonkui Green Ezekiel |
| author_facet | Mpho Mamphoka Nchabeleng Thierry Youmbi Fonkui Green Ezekiel |
| author_sort | Mpho Mamphoka Nchabeleng |
| collection | DOAJ |
| description | The efficacy of 23 bacterial isolates obtained from surface-sterilized stems and leaves of three medicinal plants (<i>Aloe barbadensis</i> Miller, <i>Artemisia afra</i>, and <i>Moringa oleifera</i>) was investigated in an endeavour to prevent the growth of <i>Mycobacterium bovis</i> using the cross-streak method. Endophytes were isolated by incubating sterile plant materials on nutrient agar at 30 °C for 5 days. Two isolates showing activity were subsequently utilized to produce the extracts. Whole-genome sequencing (WGC) was used to identify the isolates. Secondary metabolites produced after 7 days of growth in nutrient broth were harvested through extraction with ethyl acetate. The extracts were chemically profiled using gas chromatography–high resolution time-of-flight mass spectrometry (GC–HRTOF-MS). NCBI BLAST search results revealed that the isolated endophytes belonged to the Pseudomonas and Enterobacter genera, based on WGC. Two endophytes, Aloe I4 and Aloe I3–I5 from <i>Aloe barbadensis</i>, exhibited potency based on the cross-streak method. The metabolite profiling of the selected endophytes identified 34 metabolites from Aloe I4, including ergotamine, octadecane, L-proline and 143 other metabolites including quinoline and valeramide, which inhibit microbial quorum sensing. These findings suggest that bacterial endophytes from medicinal plants, particularly <i>Aloe barbadensis,</i> hold promise as sources of antimycobacterial agents for human health applications. |
| first_indexed | 2024-04-24T17:58:38Z |
| format | Article |
| id | doaj.art-c1b2818f3de642e7a7e088421b459b30 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-24T17:58:38Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c1b2818f3de642e7a7e088421b459b302024-03-27T13:56:58ZengMDPI AGMolecules1420-30492024-03-01296129710.3390/molecules29061297The Indiscriminate Chemical Makeup of Secondary Metabolites Derived from Endophytes Harvested from <i>Aloe barbadensis</i> Miller in South Africa’s Limpopo RegionMpho Mamphoka Nchabeleng0Thierry Youmbi Fonkui1Green Ezekiel2Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, Doornfontein Campus, Johannesburg 2028, South AfricaDepartment of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, Doornfontein Campus, Johannesburg 2028, South AfricaDepartment of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, Doornfontein Campus, Johannesburg 2028, South AfricaThe efficacy of 23 bacterial isolates obtained from surface-sterilized stems and leaves of three medicinal plants (<i>Aloe barbadensis</i> Miller, <i>Artemisia afra</i>, and <i>Moringa oleifera</i>) was investigated in an endeavour to prevent the growth of <i>Mycobacterium bovis</i> using the cross-streak method. Endophytes were isolated by incubating sterile plant materials on nutrient agar at 30 °C for 5 days. Two isolates showing activity were subsequently utilized to produce the extracts. Whole-genome sequencing (WGC) was used to identify the isolates. Secondary metabolites produced after 7 days of growth in nutrient broth were harvested through extraction with ethyl acetate. The extracts were chemically profiled using gas chromatography–high resolution time-of-flight mass spectrometry (GC–HRTOF-MS). NCBI BLAST search results revealed that the isolated endophytes belonged to the Pseudomonas and Enterobacter genera, based on WGC. Two endophytes, Aloe I4 and Aloe I3–I5 from <i>Aloe barbadensis</i>, exhibited potency based on the cross-streak method. The metabolite profiling of the selected endophytes identified 34 metabolites from Aloe I4, including ergotamine, octadecane, L-proline and 143 other metabolites including quinoline and valeramide, which inhibit microbial quorum sensing. These findings suggest that bacterial endophytes from medicinal plants, particularly <i>Aloe barbadensis,</i> hold promise as sources of antimycobacterial agents for human health applications.https://www.mdpi.com/1420-3049/29/6/1297endophytessecondary metabolites<i>Mycobacterium bovis</i>tuberculosismedicinal plants |
| spellingShingle | Mpho Mamphoka Nchabeleng Thierry Youmbi Fonkui Green Ezekiel The Indiscriminate Chemical Makeup of Secondary Metabolites Derived from Endophytes Harvested from <i>Aloe barbadensis</i> Miller in South Africa’s Limpopo Region Molecules endophytes secondary metabolites <i>Mycobacterium bovis</i> tuberculosis medicinal plants |
| title | The Indiscriminate Chemical Makeup of Secondary Metabolites Derived from Endophytes Harvested from <i>Aloe barbadensis</i> Miller in South Africa’s Limpopo Region |
| title_full | The Indiscriminate Chemical Makeup of Secondary Metabolites Derived from Endophytes Harvested from <i>Aloe barbadensis</i> Miller in South Africa’s Limpopo Region |
| title_fullStr | The Indiscriminate Chemical Makeup of Secondary Metabolites Derived from Endophytes Harvested from <i>Aloe barbadensis</i> Miller in South Africa’s Limpopo Region |
| title_full_unstemmed | The Indiscriminate Chemical Makeup of Secondary Metabolites Derived from Endophytes Harvested from <i>Aloe barbadensis</i> Miller in South Africa’s Limpopo Region |
| title_short | The Indiscriminate Chemical Makeup of Secondary Metabolites Derived from Endophytes Harvested from <i>Aloe barbadensis</i> Miller in South Africa’s Limpopo Region |
| title_sort | indiscriminate chemical makeup of secondary metabolites derived from endophytes harvested from i aloe barbadensis i miller in south africa s limpopo region |
| topic | endophytes secondary metabolites <i>Mycobacterium bovis</i> tuberculosis medicinal plants |
| url | https://www.mdpi.com/1420-3049/29/6/1297 |
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