The healing effects of the topical mesenchymal stem cells application on colonic anastomosis subjected to ischemia reperfusion injury
Intestinal ischemia reperfusion injury (IRI) is a challenging problem and it adversely affects the healing of colonic anastomosis. Our experimental study aimed to investigate the role of mesenchymal stem cells (MSC) administration in the healing of colonic anastomosis. A total of 33 rats were groupe...
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Kafkas University, Faculty of Veterinary Medicine
2021-08-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2831 |
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| author | Hüseyin ÖZDEN Gökhan KARACA Huri BULUT Mehmet NİYAZ Muhammed GÖMEÇ Çağatay E. DAPHAN |
| author_facet | Hüseyin ÖZDEN Gökhan KARACA Huri BULUT Mehmet NİYAZ Muhammed GÖMEÇ Çağatay E. DAPHAN |
| author_sort | Hüseyin ÖZDEN |
| collection | DOAJ |
| description | Intestinal ischemia reperfusion injury (IRI) is a challenging problem and it adversely affects the healing of colonic anastomosis.
Our experimental study aimed to investigate the role of mesenchymal stem cells (MSC) administration in the healing of colonic
anastomosis. A total of 33 rats were grouped as Control, IRI and MSC treatment groups. Three rats were reserved for obtaining MSCs.
Colonic resection and anastomosis procedure was performed in all groups. Anastomotic line was wrapped with MSCs impregnated
spongostan after colonic anastomosis in the rats of the MSC treatment group. All rats were sacrificed and anastomotic line were
sampled for examination on the post operative seventh day. Tissue hydroxyproline (HP) levels and anastomotic bursting pressures
were statistically compared. Anastomotic bursting pressures were found to be significantly high in MSC treatment group rats. The
lowest anastomotic bursting pressure was detected in IRI group rats. Hydroxyproline content of the anastomotic sites were also found
to be significantly higher in the rats of the MSC treatment group when compared with the IRI group rats. Our study showed that the
detrimental effects of IRI on the healing process of colonic anastomosis in an experimental model may be alleviated with the treatment
of MSCs. |
| first_indexed | 2024-03-13T04:39:40Z |
| format | Article |
| id | doaj.art-c1b3af5bd3c844118e709318bcb4601c |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:39:40Z |
| publishDate | 2021-08-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-c1b3af5bd3c844118e709318bcb4601c2023-06-19T06:29:11ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512021-08-0127559560010.9775/kvfd.2021.259352831The healing effects of the topical mesenchymal stem cells application on colonic anastomosis subjected to ischemia reperfusion injuryHüseyin ÖZDEN0Gökhan KARACA1Huri BULUT2Mehmet NİYAZ3Muhammed GÖMEÇ4Çağatay E. DAPHAN5Ahi Evran University Faculty of Medicine Department of General Surgery, TR-40100 Kırşehir - TURKEYKırıkkale University Faculty of Medicine Department of General Surgery, TR-71450 Kırıkkale - TURKEYİstinye University Faculty of Medicine Department of Medical Biochemistry, TR-34460 Istanbul - TURKEYBaşkent University Faculty of Medicine Department of Medical Genetics, TR-06790 Ankara - TURKEYCumhuriyet University Faculty of Medicine Department of General Surgery, TR-58140 Sivas - TURKEYKırıkkale University Faculty of Medicine Department of General Surgery, TR-71450 Kırıkkale - TURKEYIntestinal ischemia reperfusion injury (IRI) is a challenging problem and it adversely affects the healing of colonic anastomosis. Our experimental study aimed to investigate the role of mesenchymal stem cells (MSC) administration in the healing of colonic anastomosis. A total of 33 rats were grouped as Control, IRI and MSC treatment groups. Three rats were reserved for obtaining MSCs. Colonic resection and anastomosis procedure was performed in all groups. Anastomotic line was wrapped with MSCs impregnated spongostan after colonic anastomosis in the rats of the MSC treatment group. All rats were sacrificed and anastomotic line were sampled for examination on the post operative seventh day. Tissue hydroxyproline (HP) levels and anastomotic bursting pressures were statistically compared. Anastomotic bursting pressures were found to be significantly high in MSC treatment group rats. The lowest anastomotic bursting pressure was detected in IRI group rats. Hydroxyproline content of the anastomotic sites were also found to be significantly higher in the rats of the MSC treatment group when compared with the IRI group rats. Our study showed that the detrimental effects of IRI on the healing process of colonic anastomosis in an experimental model may be alleviated with the treatment of MSCs.https://vetdergikafkas.org/pdf.php?id=2831anastomotic leakagecolonic anastomosishydroxyprolinei̇schemia reperfusion injurymesenchymal stem cell |
| spellingShingle | Hüseyin ÖZDEN Gökhan KARACA Huri BULUT Mehmet NİYAZ Muhammed GÖMEÇ Çağatay E. DAPHAN The healing effects of the topical mesenchymal stem cells application on colonic anastomosis subjected to ischemia reperfusion injury Kafkas Universitesi Veteriner Fakültesi Dergisi anastomotic leakage colonic anastomosis hydroxyproline i̇schemia reperfusion injury mesenchymal stem cell |
| title | The healing effects of the topical mesenchymal stem cells application on colonic anastomosis subjected to ischemia reperfusion injury |
| title_full | The healing effects of the topical mesenchymal stem cells application on colonic anastomosis subjected to ischemia reperfusion injury |
| title_fullStr | The healing effects of the topical mesenchymal stem cells application on colonic anastomosis subjected to ischemia reperfusion injury |
| title_full_unstemmed | The healing effects of the topical mesenchymal stem cells application on colonic anastomosis subjected to ischemia reperfusion injury |
| title_short | The healing effects of the topical mesenchymal stem cells application on colonic anastomosis subjected to ischemia reperfusion injury |
| title_sort | healing effects of the topical mesenchymal stem cells application on colonic anastomosis subjected to ischemia reperfusion injury |
| topic | anastomotic leakage colonic anastomosis hydroxyproline i̇schemia reperfusion injury mesenchymal stem cell |
| url | https://vetdergikafkas.org/pdf.php?id=2831 |
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