Preparative Purification of Anti-Proliferative Diarylheptanoids from Betula platyphylla by High-Speed Counter-Current Chromatography
A simple and rapid method using high-speed counter-current chromatography (HSCCC), along with bioassay-guided fractionation based on the anti-proliferative activity against renal and colon cancer cells, has been developed for the preparative separation of aceroside VIII (1) and platyphylloside (2) f...
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MDPI AG
2016-05-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/21/6/700 |
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| author | Namki Cho Hyun Woo Kim Tae Bum Kim Tanya T. Ransom John A. Beutler Sang Hyun Sung |
| author_facet | Namki Cho Hyun Woo Kim Tae Bum Kim Tanya T. Ransom John A. Beutler Sang Hyun Sung |
| author_sort | Namki Cho |
| collection | DOAJ |
| description | A simple and rapid method using high-speed counter-current chromatography (HSCCC), along with bioassay-guided fractionation based on the anti-proliferative activity against renal and colon cancer cells, has been developed for the preparative separation of aceroside VIII (1) and platyphylloside (2) from Betula platyphylla. A solvent system composed of ethyl acetate/acetonitrile/water (1:0.1:1, v/v/v) was optimized for the separation. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase. Among these isolated diarylheptanoids, platyphylloside (2) showed anti-proliferative activity in the COLO205 and KM12 colon cells and renal cancer cell lines A498, U031, as well as in MG63 and MG 63.3 osteosarcoma cells. In addition, it showed dose dependent inhibitory effects in the NCI 60 cell line assay. These results suggest that the diarylheptanoids isolated from B. platyphylla with an efficient HSCCC method could be potential multi-targeted therapeutic agents for cancer. |
| first_indexed | 2024-12-19T03:52:00Z |
| format | Article |
| id | doaj.art-c1b3b661d9144832bd8f69dfd326ca69 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-19T03:52:00Z |
| publishDate | 2016-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c1b3b661d9144832bd8f69dfd326ca692022-12-21T20:36:56ZengMDPI AGMolecules1420-30492016-05-0121670010.3390/molecules21060700molecules21060700Preparative Purification of Anti-Proliferative Diarylheptanoids from Betula platyphylla by High-Speed Counter-Current ChromatographyNamki Cho0Hyun Woo Kim1Tae Bum Kim2Tanya T. Ransom3John A. Beutler4Sang Hyun Sung5College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 08826, KoreaCollege of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 08826, KoreaCollege of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 08826, KoreaMolecular Targets Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702-1202, USAMolecular Targets Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702-1202, USACollege of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 08826, KoreaA simple and rapid method using high-speed counter-current chromatography (HSCCC), along with bioassay-guided fractionation based on the anti-proliferative activity against renal and colon cancer cells, has been developed for the preparative separation of aceroside VIII (1) and platyphylloside (2) from Betula platyphylla. A solvent system composed of ethyl acetate/acetonitrile/water (1:0.1:1, v/v/v) was optimized for the separation. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase. Among these isolated diarylheptanoids, platyphylloside (2) showed anti-proliferative activity in the COLO205 and KM12 colon cells and renal cancer cell lines A498, U031, as well as in MG63 and MG 63.3 osteosarcoma cells. In addition, it showed dose dependent inhibitory effects in the NCI 60 cell line assay. These results suggest that the diarylheptanoids isolated from B. platyphylla with an efficient HSCCC method could be potential multi-targeted therapeutic agents for cancer.http://www.mdpi.com/1420-3049/21/6/700HSCCCdiarylheptanoidanti-proliferation |
| spellingShingle | Namki Cho Hyun Woo Kim Tae Bum Kim Tanya T. Ransom John A. Beutler Sang Hyun Sung Preparative Purification of Anti-Proliferative Diarylheptanoids from Betula platyphylla by High-Speed Counter-Current Chromatography Molecules HSCCC diarylheptanoid anti-proliferation |
| title | Preparative Purification of Anti-Proliferative Diarylheptanoids from Betula platyphylla by High-Speed Counter-Current Chromatography |
| title_full | Preparative Purification of Anti-Proliferative Diarylheptanoids from Betula platyphylla by High-Speed Counter-Current Chromatography |
| title_fullStr | Preparative Purification of Anti-Proliferative Diarylheptanoids from Betula platyphylla by High-Speed Counter-Current Chromatography |
| title_full_unstemmed | Preparative Purification of Anti-Proliferative Diarylheptanoids from Betula platyphylla by High-Speed Counter-Current Chromatography |
| title_short | Preparative Purification of Anti-Proliferative Diarylheptanoids from Betula platyphylla by High-Speed Counter-Current Chromatography |
| title_sort | preparative purification of anti proliferative diarylheptanoids from betula platyphylla by high speed counter current chromatography |
| topic | HSCCC diarylheptanoid anti-proliferation |
| url | http://www.mdpi.com/1420-3049/21/6/700 |
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