E3 Ubiquitin Ligase PUB23 in Kiwifruit Interacts with Trihelix Transcription Factor GT1 and Negatively Regulates Immune Responses against <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>
Kiwifruit bacterial canker caused by <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> (Psa) is the most serious disease threatening kiwifruit production. Our previous study found genes encoding the U-box containing proteins were significantly regulated by Psa infection. Here...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/25/3/1930 |
| Summary: | Kiwifruit bacterial canker caused by <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> (Psa) is the most serious disease threatening kiwifruit production. Our previous study found genes encoding the U-box containing proteins were significantly regulated by Psa infection. Here, we report a U-box type E3 ubiquitin ligase PUB23 in kiwifruit which acts as a negative regulator of immune responses against Psa. PUB23 was found to physically interact with GT1, a trihelix transcription factor, in vitro and in vivo. The expression of <i>GT1</i> was up-regulated in <i>PUB23</i>-silenced plants, indicating that interacting with PUB23 may directly or indirectly suppress <i>GT1</i> expression. The silencing of <i>PUB23</i> led to enhanced immune responses of PAMP-triggered immunity (PTI), including a higher expression level of defense marker genes <i>PR1</i> and <i>RIN4</i>, and increased accumulation of hydrogen peroxide and superoxide anion. Our results reveal a negative role PUB23 plays in kiwifruit immune responses against Psa and may regulate gene expression by interacting with GT1. |
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| ISSN: | 1661-6596 1422-0067 |