E3 Ubiquitin Ligase PUB23 in Kiwifruit Interacts with Trihelix Transcription Factor GT1 and Negatively Regulates Immune Responses against <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>
Kiwifruit bacterial canker caused by <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> (Psa) is the most serious disease threatening kiwifruit production. Our previous study found genes encoding the U-box containing proteins were significantly regulated by Psa infection. Here...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/3/1930 |
| _version_ | 1797318621844733952 |
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| author | Tao Wang Gang Wang Jiyu Zhang Jiping Xuan |
| author_facet | Tao Wang Gang Wang Jiyu Zhang Jiping Xuan |
| author_sort | Tao Wang |
| collection | DOAJ |
| description | Kiwifruit bacterial canker caused by <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> (Psa) is the most serious disease threatening kiwifruit production. Our previous study found genes encoding the U-box containing proteins were significantly regulated by Psa infection. Here, we report a U-box type E3 ubiquitin ligase PUB23 in kiwifruit which acts as a negative regulator of immune responses against Psa. PUB23 was found to physically interact with GT1, a trihelix transcription factor, in vitro and in vivo. The expression of <i>GT1</i> was up-regulated in <i>PUB23</i>-silenced plants, indicating that interacting with PUB23 may directly or indirectly suppress <i>GT1</i> expression. The silencing of <i>PUB23</i> led to enhanced immune responses of PAMP-triggered immunity (PTI), including a higher expression level of defense marker genes <i>PR1</i> and <i>RIN4</i>, and increased accumulation of hydrogen peroxide and superoxide anion. Our results reveal a negative role PUB23 plays in kiwifruit immune responses against Psa and may regulate gene expression by interacting with GT1. |
| first_indexed | 2024-03-08T03:54:59Z |
| format | Article |
| id | doaj.art-c1b619b47f2d419cbee225a57c277ec4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T03:54:59Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c1b619b47f2d419cbee225a57c277ec42024-02-09T15:15:15ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01253193010.3390/ijms25031930E3 Ubiquitin Ligase PUB23 in Kiwifruit Interacts with Trihelix Transcription Factor GT1 and Negatively Regulates Immune Responses against <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>Tao Wang0Gang Wang1Jiyu Zhang2Jiping Xuan3Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaKiwifruit bacterial canker caused by <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> (Psa) is the most serious disease threatening kiwifruit production. Our previous study found genes encoding the U-box containing proteins were significantly regulated by Psa infection. Here, we report a U-box type E3 ubiquitin ligase PUB23 in kiwifruit which acts as a negative regulator of immune responses against Psa. PUB23 was found to physically interact with GT1, a trihelix transcription factor, in vitro and in vivo. The expression of <i>GT1</i> was up-regulated in <i>PUB23</i>-silenced plants, indicating that interacting with PUB23 may directly or indirectly suppress <i>GT1</i> expression. The silencing of <i>PUB23</i> led to enhanced immune responses of PAMP-triggered immunity (PTI), including a higher expression level of defense marker genes <i>PR1</i> and <i>RIN4</i>, and increased accumulation of hydrogen peroxide and superoxide anion. Our results reveal a negative role PUB23 plays in kiwifruit immune responses against Psa and may regulate gene expression by interacting with GT1.https://www.mdpi.com/1422-0067/25/3/1930U-boxprotein interactionbacterial cankerimmunity |
| spellingShingle | Tao Wang Gang Wang Jiyu Zhang Jiping Xuan E3 Ubiquitin Ligase PUB23 in Kiwifruit Interacts with Trihelix Transcription Factor GT1 and Negatively Regulates Immune Responses against <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> International Journal of Molecular Sciences U-box protein interaction bacterial canker immunity |
| title | E3 Ubiquitin Ligase PUB23 in Kiwifruit Interacts with Trihelix Transcription Factor GT1 and Negatively Regulates Immune Responses against <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> |
| title_full | E3 Ubiquitin Ligase PUB23 in Kiwifruit Interacts with Trihelix Transcription Factor GT1 and Negatively Regulates Immune Responses against <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> |
| title_fullStr | E3 Ubiquitin Ligase PUB23 in Kiwifruit Interacts with Trihelix Transcription Factor GT1 and Negatively Regulates Immune Responses against <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> |
| title_full_unstemmed | E3 Ubiquitin Ligase PUB23 in Kiwifruit Interacts with Trihelix Transcription Factor GT1 and Negatively Regulates Immune Responses against <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> |
| title_short | E3 Ubiquitin Ligase PUB23 in Kiwifruit Interacts with Trihelix Transcription Factor GT1 and Negatively Regulates Immune Responses against <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> |
| title_sort | e3 ubiquitin ligase pub23 in kiwifruit interacts with trihelix transcription factor gt1 and negatively regulates immune responses against i pseudomonas syringae i pv i actinidiae i |
| topic | U-box protein interaction bacterial canker immunity |
| url | https://www.mdpi.com/1422-0067/25/3/1930 |
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