The Pelagic Bacterium Paraphotobacterium marinum Has the Smallest Complete Genome Within the Family Vibrionaceae
Members of the family Vibrionaceae are metabolically versatile and ubiquitous in natural environments, with extraordinary genome feature of two chromosomes. Here we reported the complete genome of Paraphotobacterium marinum NSCS20N07DT, a recently described novel genus-level species in the family Vi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01994/full |
| _version_ | 1818653060624285696 |
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| author | Zhaobin Huang Zhaobin Huang Zhaobin Huang Zhaobin Huang Zhen Liu Zongze Shao Zongze Shao Zongze Shao Zongze Shao |
| author_facet | Zhaobin Huang Zhaobin Huang Zhaobin Huang Zhaobin Huang Zhen Liu Zongze Shao Zongze Shao Zongze Shao Zongze Shao |
| author_sort | Zhaobin Huang |
| collection | DOAJ |
| description | Members of the family Vibrionaceae are metabolically versatile and ubiquitous in natural environments, with extraordinary genome feature of two chromosomes. Here we reported the complete genome of Paraphotobacterium marinum NSCS20N07DT, a recently described novel genus-level species in the family Vibrionaceae. It contained two circular chromosomes with a size of 2,593,992 bp with G+C content of 31.2 mol%, and a plasmid with a size of 5,539 bp. The larger chromosome (Chr. I) had a genome size of 1,426,504 bp with G+C content of 31.6 mol%, and the smaller one (Chr. II) had a genome size of 1,161,949 bp with G+C content of 30.8 mol%. The two chromosomes have strikingly similar G+C contents with difference of <1% and similar percentages of coding regions. Interestingly, by comparison to 134 species affiliated with seven genera within the family Vibrionaceae, P. marinum NSCS20N07DT possessed the smallest genome size and lowest G+C content. Clusters of orthologous groups of proteins functional categories revealed that the two chromosomes had different distributions of functional classes, indicating they take different cellular functions. Surprisingly, Chr. II had a large proportion of unknown genes than Chr. I. Metabolic characteristics predicted that Chr. I performed the essential metabolism, which can be complemented by the Chr. II, such as amino acids biosynthesis. Microbial community analysis of in situ surface seawater revealed that P. marinum accounted for one to four sequences among more than 20,000 of 16S ribosomal RNA gene V4 contigs, representing it apparently appeared as a rare species. What’s more, P. marinum was anticipated to be specific to the pelagic ocean. This study will provide new insight into more understanding the genomic and metabolic features of multiple chromosomes in prokaryote and emphasize the ecological distribution of the members in the family Vibrionaceae as a rare species. |
| first_indexed | 2024-12-17T02:31:54Z |
| format | Article |
| id | doaj.art-c1ba4c6aaf6547e4aaa26bba4bec6a06 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-17T02:31:54Z |
| publishDate | 2017-10-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-c1ba4c6aaf6547e4aaa26bba4bec6a062022-12-21T22:06:56ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-10-01810.3389/fmicb.2017.01994296904The Pelagic Bacterium Paraphotobacterium marinum Has the Smallest Complete Genome Within the Family VibrionaceaeZhaobin Huang0Zhaobin Huang1Zhaobin Huang2Zhaobin Huang3Zhen Liu4Zongze Shao5Zongze Shao6Zongze Shao7Zongze Shao8Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, State of Oceanic Administration, Xiamen, ChinaFujian Key Laboratory of Marine Genetic Resources, Xiamen, ChinaFujian Collaborative Innovation Center of Marine Biological Resources, Xiamen, ChinaSouth China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen, ChinaShanghai Majorbio Bio-Pharm Biotechnology Co., Ltd., Shanghai, ChinaKey Laboratory of Marine Genetic Resources, Third Institute of Oceanography, State of Oceanic Administration, Xiamen, ChinaFujian Key Laboratory of Marine Genetic Resources, Xiamen, ChinaFujian Collaborative Innovation Center of Marine Biological Resources, Xiamen, ChinaSouth China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen, ChinaMembers of the family Vibrionaceae are metabolically versatile and ubiquitous in natural environments, with extraordinary genome feature of two chromosomes. Here we reported the complete genome of Paraphotobacterium marinum NSCS20N07DT, a recently described novel genus-level species in the family Vibrionaceae. It contained two circular chromosomes with a size of 2,593,992 bp with G+C content of 31.2 mol%, and a plasmid with a size of 5,539 bp. The larger chromosome (Chr. I) had a genome size of 1,426,504 bp with G+C content of 31.6 mol%, and the smaller one (Chr. II) had a genome size of 1,161,949 bp with G+C content of 30.8 mol%. The two chromosomes have strikingly similar G+C contents with difference of <1% and similar percentages of coding regions. Interestingly, by comparison to 134 species affiliated with seven genera within the family Vibrionaceae, P. marinum NSCS20N07DT possessed the smallest genome size and lowest G+C content. Clusters of orthologous groups of proteins functional categories revealed that the two chromosomes had different distributions of functional classes, indicating they take different cellular functions. Surprisingly, Chr. II had a large proportion of unknown genes than Chr. I. Metabolic characteristics predicted that Chr. I performed the essential metabolism, which can be complemented by the Chr. II, such as amino acids biosynthesis. Microbial community analysis of in situ surface seawater revealed that P. marinum accounted for one to four sequences among more than 20,000 of 16S ribosomal RNA gene V4 contigs, representing it apparently appeared as a rare species. What’s more, P. marinum was anticipated to be specific to the pelagic ocean. This study will provide new insight into more understanding the genomic and metabolic features of multiple chromosomes in prokaryote and emphasize the ecological distribution of the members in the family Vibrionaceae as a rare species.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01994/fullParaphotobacterium marinumcomplete genometwo chromosomesmetabolic featuresrare species |
| spellingShingle | Zhaobin Huang Zhaobin Huang Zhaobin Huang Zhaobin Huang Zhen Liu Zongze Shao Zongze Shao Zongze Shao Zongze Shao The Pelagic Bacterium Paraphotobacterium marinum Has the Smallest Complete Genome Within the Family Vibrionaceae Frontiers in Microbiology Paraphotobacterium marinum complete genome two chromosomes metabolic features rare species |
| title | The Pelagic Bacterium Paraphotobacterium marinum Has the Smallest Complete Genome Within the Family Vibrionaceae |
| title_full | The Pelagic Bacterium Paraphotobacterium marinum Has the Smallest Complete Genome Within the Family Vibrionaceae |
| title_fullStr | The Pelagic Bacterium Paraphotobacterium marinum Has the Smallest Complete Genome Within the Family Vibrionaceae |
| title_full_unstemmed | The Pelagic Bacterium Paraphotobacterium marinum Has the Smallest Complete Genome Within the Family Vibrionaceae |
| title_short | The Pelagic Bacterium Paraphotobacterium marinum Has the Smallest Complete Genome Within the Family Vibrionaceae |
| title_sort | pelagic bacterium paraphotobacterium marinum has the smallest complete genome within the family vibrionaceae |
| topic | Paraphotobacterium marinum complete genome two chromosomes metabolic features rare species |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01994/full |
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