Caffeine Administration Mitigates Chronic Stress-Induced Behavioral Deficits, Neurochemical Alterations, and Glial Disruptions in Rats
Prolonged exposure to stress has detrimental effects on health, and the consumption of caffeine, mostly contained in energy drinks, has become a widely adopted stress coping strategy. Currently, there is limited information regarding the effects of caffeine intake on chronic stress exposure. Thus, t...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Brain Sciences |
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| Online Access: | https://www.mdpi.com/2076-3425/13/12/1663 |
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| author | Oritoke M. Okeowo Olanrewaju O. Oke Gloria O. David Omamuyovwi M. Ijomone |
| author_facet | Oritoke M. Okeowo Olanrewaju O. Oke Gloria O. David Omamuyovwi M. Ijomone |
| author_sort | Oritoke M. Okeowo |
| collection | DOAJ |
| description | Prolonged exposure to stress has detrimental effects on health, and the consumption of caffeine, mostly contained in energy drinks, has become a widely adopted stress coping strategy. Currently, there is limited information regarding the effects of caffeine intake on chronic stress exposure. Thus, this study investigated the effects of caffeine administration on chronic stress-induced behavioral deficits, neurochemical alterations, and glial disruptions in experimental rats. Thirty male Wistar rats were randomly assigned to five groups (n = 6): non-stress control, stress control, and caffeine groups of doses 12.5, 25, and 50 mg/kg. The stress control and caffeine groups were subjected to an unpredictable chronic mild stress (UCMS) protocol daily for 14 days. The rats were evaluated for phenotypic and neurobehavioral assessments. Thereafter, the rat brains were processed for biochemical and immunohistochemical assays. Caffeine administration was found to ameliorate behavioral dysfunctions in rats exposed to UCMS. The UCMS-induced changes in brain levels of monoamines, cholinesterases, and some oxidative stress biomarkers were reversed by caffeine. Caffeine administration also produced mild protective effects against UCMS-induced changes in GFAP and Iba-1 expression in stress-specific brain regions. These results showed that low and moderate doses of caffeine reversed most of the stress-induced changes, suggesting its ameliorative potential against chronic stress-induced alterations. |
| first_indexed | 2024-03-08T20:57:04Z |
| format | Article |
| id | doaj.art-c1bd6fa886ef4f3da731111923dc4ca3 |
| institution | Directory Open Access Journal |
| issn | 2076-3425 |
| language | English |
| last_indexed | 2024-03-08T20:57:04Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Brain Sciences |
| spelling | doaj.art-c1bd6fa886ef4f3da731111923dc4ca32023-12-22T13:57:08ZengMDPI AGBrain Sciences2076-34252023-11-011312166310.3390/brainsci13121663Caffeine Administration Mitigates Chronic Stress-Induced Behavioral Deficits, Neurochemical Alterations, and Glial Disruptions in RatsOritoke M. Okeowo0Olanrewaju O. Oke1Gloria O. David2Omamuyovwi M. Ijomone3Department of Physiology, School of Basic Medical Sciences, Federal University of Technology, Akure 340252, Ondo State, NigeriaDepartment of Physiology, School of Basic Medical Sciences, Federal University of Technology, Akure 340252, Ondo State, NigeriaDepartment of Anatomy, School of Basic Medical Sciences, Federal University of Technology, Akure 340252, Ondo State, NigeriaLaboratory for Experimental and Translational Neurobiology, University of Medical Sciences, Ondo 351101, Ondo State, NigeriaProlonged exposure to stress has detrimental effects on health, and the consumption of caffeine, mostly contained in energy drinks, has become a widely adopted stress coping strategy. Currently, there is limited information regarding the effects of caffeine intake on chronic stress exposure. Thus, this study investigated the effects of caffeine administration on chronic stress-induced behavioral deficits, neurochemical alterations, and glial disruptions in experimental rats. Thirty male Wistar rats were randomly assigned to five groups (n = 6): non-stress control, stress control, and caffeine groups of doses 12.5, 25, and 50 mg/kg. The stress control and caffeine groups were subjected to an unpredictable chronic mild stress (UCMS) protocol daily for 14 days. The rats were evaluated for phenotypic and neurobehavioral assessments. Thereafter, the rat brains were processed for biochemical and immunohistochemical assays. Caffeine administration was found to ameliorate behavioral dysfunctions in rats exposed to UCMS. The UCMS-induced changes in brain levels of monoamines, cholinesterases, and some oxidative stress biomarkers were reversed by caffeine. Caffeine administration also produced mild protective effects against UCMS-induced changes in GFAP and Iba-1 expression in stress-specific brain regions. These results showed that low and moderate doses of caffeine reversed most of the stress-induced changes, suggesting its ameliorative potential against chronic stress-induced alterations.https://www.mdpi.com/2076-3425/13/12/1663caffeineunpredictable chronic mild stressbehaviorGFAPIba-1 |
| spellingShingle | Oritoke M. Okeowo Olanrewaju O. Oke Gloria O. David Omamuyovwi M. Ijomone Caffeine Administration Mitigates Chronic Stress-Induced Behavioral Deficits, Neurochemical Alterations, and Glial Disruptions in Rats Brain Sciences caffeine unpredictable chronic mild stress behavior GFAP Iba-1 |
| title | Caffeine Administration Mitigates Chronic Stress-Induced Behavioral Deficits, Neurochemical Alterations, and Glial Disruptions in Rats |
| title_full | Caffeine Administration Mitigates Chronic Stress-Induced Behavioral Deficits, Neurochemical Alterations, and Glial Disruptions in Rats |
| title_fullStr | Caffeine Administration Mitigates Chronic Stress-Induced Behavioral Deficits, Neurochemical Alterations, and Glial Disruptions in Rats |
| title_full_unstemmed | Caffeine Administration Mitigates Chronic Stress-Induced Behavioral Deficits, Neurochemical Alterations, and Glial Disruptions in Rats |
| title_short | Caffeine Administration Mitigates Chronic Stress-Induced Behavioral Deficits, Neurochemical Alterations, and Glial Disruptions in Rats |
| title_sort | caffeine administration mitigates chronic stress induced behavioral deficits neurochemical alterations and glial disruptions in rats |
| topic | caffeine unpredictable chronic mild stress behavior GFAP Iba-1 |
| url | https://www.mdpi.com/2076-3425/13/12/1663 |
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