Phytotoxicity and Other Adverse Effects on the In Vitro Shoot Cultures Caused by Virus Elimination Treatments: Reasons and Solutions
In general, in vitro virus elimination is based on the culture of isolated meristem, and in addition thermotherapy, chemotherapy, electrotherapy, and cryotherapy can also be applied. During these processes, plantlets suffer several stresses, which can result in low rate of survival, inhibited growth...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/10/4/670 |
| _version_ | 1797539423668142080 |
|---|---|
| author | Katalin Magyar-Tábori Nóra Mendler-Drienyovszki Alexandra Hanász László Zsombik Judit Dobránszki |
| author_facet | Katalin Magyar-Tábori Nóra Mendler-Drienyovszki Alexandra Hanász László Zsombik Judit Dobránszki |
| author_sort | Katalin Magyar-Tábori |
| collection | DOAJ |
| description | In general, in vitro virus elimination is based on the culture of isolated meristem, and in addition thermotherapy, chemotherapy, electrotherapy, and cryotherapy can also be applied. During these processes, plantlets suffer several stresses, which can result in low rate of survival, inhibited growth, incomplete development, or abnormal morphology. Even though the in vitro cultures survive the treatment, further development can be inhibited; thus, regeneration capacity of treated in vitro shoots or explants play also an important role in successful virus elimination. Sensitivity of genotypes to treatments is very different, and the rate of destruction largely depends on the physiological condition of plants as well. Exposure time of treatments affects the rate of damage in almost every therapy. Other factors such as temperature, illumination (thermotherapy), type and concentration of applied chemicals (chemo- and cryotherapy), and electric current intensity (electrotherapy) also may have a great impact on the rate of damage. However, there are several ways to decrease the harmful effect of treatments. This review summarizes the harmful effects of virus elimination treatments applied on tissue cultures reported in the literature. The aim of this review is to expound the solutions that can be used to mitigate phytotoxic and other adverse effects in practice. |
| first_indexed | 2024-03-10T12:44:52Z |
| format | Article |
| id | doaj.art-c1bdad9bc32b430cbe46c7ce882e62a3 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T12:44:52Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-c1bdad9bc32b430cbe46c7ce882e62a32023-11-21T13:34:39ZengMDPI AGPlants2223-77472021-03-0110467010.3390/plants10040670Phytotoxicity and Other Adverse Effects on the In Vitro Shoot Cultures Caused by Virus Elimination Treatments: Reasons and SolutionsKatalin Magyar-Tábori0Nóra Mendler-Drienyovszki1Alexandra Hanász2László Zsombik3Judit Dobránszki4Centre for Agricultural Genomics and Biotechnology, Faculty of the Agricultural and Food Science and Environmental Management, University of Debrecen, P.O. Box 12, H-4400 Nyíregyháza, HungaryResearch Institute of Nyíregyháza, Institutes for Agricultural Research and Educational Farm (IAREF), University of Debrecen, P.O. Box 12, H-4400 Nyíregyháza, HungaryKerpely Kálmán Doctoral School of Crop Production and Horticultural Sciences, University of Debrecen, Böszörményi Str. 138, H-4032 Debrecen, HungaryResearch Institute of Nyíregyháza, Institutes for Agricultural Research and Educational Farm (IAREF), University of Debrecen, P.O. Box 12, H-4400 Nyíregyháza, HungaryCentre for Agricultural Genomics and Biotechnology, Faculty of the Agricultural and Food Science and Environmental Management, University of Debrecen, P.O. Box 12, H-4400 Nyíregyháza, HungaryIn general, in vitro virus elimination is based on the culture of isolated meristem, and in addition thermotherapy, chemotherapy, electrotherapy, and cryotherapy can also be applied. During these processes, plantlets suffer several stresses, which can result in low rate of survival, inhibited growth, incomplete development, or abnormal morphology. Even though the in vitro cultures survive the treatment, further development can be inhibited; thus, regeneration capacity of treated in vitro shoots or explants play also an important role in successful virus elimination. Sensitivity of genotypes to treatments is very different, and the rate of destruction largely depends on the physiological condition of plants as well. Exposure time of treatments affects the rate of damage in almost every therapy. Other factors such as temperature, illumination (thermotherapy), type and concentration of applied chemicals (chemo- and cryotherapy), and electric current intensity (electrotherapy) also may have a great impact on the rate of damage. However, there are several ways to decrease the harmful effect of treatments. This review summarizes the harmful effects of virus elimination treatments applied on tissue cultures reported in the literature. The aim of this review is to expound the solutions that can be used to mitigate phytotoxic and other adverse effects in practice.https://www.mdpi.com/2223-7747/10/4/670virus eradicationchemotherapycryotherapyelectrotherapymeristem culturethermotherapy |
| spellingShingle | Katalin Magyar-Tábori Nóra Mendler-Drienyovszki Alexandra Hanász László Zsombik Judit Dobránszki Phytotoxicity and Other Adverse Effects on the In Vitro Shoot Cultures Caused by Virus Elimination Treatments: Reasons and Solutions Plants virus eradication chemotherapy cryotherapy electrotherapy meristem culture thermotherapy |
| title | Phytotoxicity and Other Adverse Effects on the In Vitro Shoot Cultures Caused by Virus Elimination Treatments: Reasons and Solutions |
| title_full | Phytotoxicity and Other Adverse Effects on the In Vitro Shoot Cultures Caused by Virus Elimination Treatments: Reasons and Solutions |
| title_fullStr | Phytotoxicity and Other Adverse Effects on the In Vitro Shoot Cultures Caused by Virus Elimination Treatments: Reasons and Solutions |
| title_full_unstemmed | Phytotoxicity and Other Adverse Effects on the In Vitro Shoot Cultures Caused by Virus Elimination Treatments: Reasons and Solutions |
| title_short | Phytotoxicity and Other Adverse Effects on the In Vitro Shoot Cultures Caused by Virus Elimination Treatments: Reasons and Solutions |
| title_sort | phytotoxicity and other adverse effects on the in vitro shoot cultures caused by virus elimination treatments reasons and solutions |
| topic | virus eradication chemotherapy cryotherapy electrotherapy meristem culture thermotherapy |
| url | https://www.mdpi.com/2223-7747/10/4/670 |
| work_keys_str_mv | AT katalinmagyartabori phytotoxicityandotheradverseeffectsontheinvitroshootculturescausedbyviruseliminationtreatmentsreasonsandsolutions AT noramendlerdrienyovszki phytotoxicityandotheradverseeffectsontheinvitroshootculturescausedbyviruseliminationtreatmentsreasonsandsolutions AT alexandrahanasz phytotoxicityandotheradverseeffectsontheinvitroshootculturescausedbyviruseliminationtreatmentsreasonsandsolutions AT laszlozsombik phytotoxicityandotheradverseeffectsontheinvitroshootculturescausedbyviruseliminationtreatmentsreasonsandsolutions AT juditdobranszki phytotoxicityandotheradverseeffectsontheinvitroshootculturescausedbyviruseliminationtreatmentsreasonsandsolutions |