Antitumor effect of algae silver nanoparticles on human triple negative breast cancer cells
In recent years, metallic nanoparticles have gained increasing attention due to their prospective applications in the field of nanomedicine, with increasing research into their use in cancer therapy. In this current research, we investigated the effect of green synthesized Silver Nanoparticles (AgNP...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Biomedicine & Pharmacotherapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332223013306 |
| _version_ | 1797630705883152384 |
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| author | Rawan Salih Khalid Bajou Baraah Shaker Abdelaziz Elgamouz |
| author_facet | Rawan Salih Khalid Bajou Baraah Shaker Abdelaziz Elgamouz |
| author_sort | Rawan Salih |
| collection | DOAJ |
| description | In recent years, metallic nanoparticles have gained increasing attention due to their prospective applications in the field of nanomedicine, with increasing research into their use in cancer therapy. In this current research, we investigated the effect of green synthesized Silver Nanoparticles (AgNPs) capped with Noctiluca scintillans algae extract. The phytochemicals present in the shell of AgNPs were identified using GC-MS. Different compounds with anticancer activity such as n-hexadecanoic acid, beta-sitosterol, stigmasterol and palmitic acid were detected among others. The effects of Algae-AgNPs synthesized were tested on MDA-MB-231 human breast cancer cells and HaCat human keratinocyte normal cells. Cell viability assay revealed a time and dose-dependent effect against breast cancer cells with a less potent effect against normal cells. The cell viability reduction is not attributed to a cytotoxic nor an antiproliferative effect of the Algae-AgNPs as attested by LDH release and BrdU incorporation. Algae-AgNPs exhibited an exceptional ability to specifically induce apoptosis in cancer cells and not normal cells. The observed effects are not attributed to the AgNPs, as demonstrated by the lack of impact of the Starch-AgNPs (used as a negative control) on cell survival and apoptosis. In addition to that, we show that Algae-AgNPs significantly reduced tumor cell migration by downregulation of matrix metalloprotease-9 levels. In vivo, the breast cancer xenograft model showed a significant reduction of tumor growth in mice treated with Algae-AgNPs. These findings highlight the promising potential of the green synthesized AgNPs as a safe targeted therapy for cancer treatment. |
| first_indexed | 2024-03-11T11:11:55Z |
| format | Article |
| id | doaj.art-c1be11672eec4b8c97fd179280413256 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-03-11T11:11:55Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-c1be11672eec4b8c97fd1792804132562023-11-12T04:38:38ZengElsevierBiomedicine & Pharmacotherapy0753-33222023-12-01168115532Antitumor effect of algae silver nanoparticles on human triple negative breast cancer cellsRawan Salih0Khalid Bajou1Baraah Shaker2Abdelaziz Elgamouz3Department of Applied Biology, College of Sciences, University of Sharjah, Sharjah 27272, United Arab Emirates; Human Genetics and Stem Cells Research Group, Research Institute of Science and Engineering, University of Sharjah, Sharjah 27272, United Arab EmiratesDepartment of Applied Biology, College of Sciences, University of Sharjah, Sharjah 27272, United Arab Emirates; Human Genetics and Stem Cells Research Group, Research Institute of Science and Engineering, University of Sharjah, Sharjah 27272, United Arab Emirates; Corresponding author at: Department of Applied Biology, College of Sciences, University of Sharjah, Sharjah 27272, United Arab Emirates.Department of Applied Biology, College of Sciences, University of Sharjah, Sharjah 27272, United Arab Emirates; Human Genetics and Stem Cells Research Group, Research Institute of Science and Engineering, University of Sharjah, Sharjah 27272, United Arab EmiratesDepartment of Chemistry, College of Sciences, University of Sharjah, Sharjah 27272, United Arab EmiratesIn recent years, metallic nanoparticles have gained increasing attention due to their prospective applications in the field of nanomedicine, with increasing research into their use in cancer therapy. In this current research, we investigated the effect of green synthesized Silver Nanoparticles (AgNPs) capped with Noctiluca scintillans algae extract. The phytochemicals present in the shell of AgNPs were identified using GC-MS. Different compounds with anticancer activity such as n-hexadecanoic acid, beta-sitosterol, stigmasterol and palmitic acid were detected among others. The effects of Algae-AgNPs synthesized were tested on MDA-MB-231 human breast cancer cells and HaCat human keratinocyte normal cells. Cell viability assay revealed a time and dose-dependent effect against breast cancer cells with a less potent effect against normal cells. The cell viability reduction is not attributed to a cytotoxic nor an antiproliferative effect of the Algae-AgNPs as attested by LDH release and BrdU incorporation. Algae-AgNPs exhibited an exceptional ability to specifically induce apoptosis in cancer cells and not normal cells. The observed effects are not attributed to the AgNPs, as demonstrated by the lack of impact of the Starch-AgNPs (used as a negative control) on cell survival and apoptosis. In addition to that, we show that Algae-AgNPs significantly reduced tumor cell migration by downregulation of matrix metalloprotease-9 levels. In vivo, the breast cancer xenograft model showed a significant reduction of tumor growth in mice treated with Algae-AgNPs. These findings highlight the promising potential of the green synthesized AgNPs as a safe targeted therapy for cancer treatment.http://www.sciencedirect.com/science/article/pii/S0753332223013306Green Silver nanoparticlesNoctiluca scintillansAnti-cancerMDA-MB-231ApoptosisMigration |
| spellingShingle | Rawan Salih Khalid Bajou Baraah Shaker Abdelaziz Elgamouz Antitumor effect of algae silver nanoparticles on human triple negative breast cancer cells Biomedicine & Pharmacotherapy Green Silver nanoparticles Noctiluca scintillans Anti-cancer MDA-MB-231 Apoptosis Migration |
| title | Antitumor effect of algae silver nanoparticles on human triple negative breast cancer cells |
| title_full | Antitumor effect of algae silver nanoparticles on human triple negative breast cancer cells |
| title_fullStr | Antitumor effect of algae silver nanoparticles on human triple negative breast cancer cells |
| title_full_unstemmed | Antitumor effect of algae silver nanoparticles on human triple negative breast cancer cells |
| title_short | Antitumor effect of algae silver nanoparticles on human triple negative breast cancer cells |
| title_sort | antitumor effect of algae silver nanoparticles on human triple negative breast cancer cells |
| topic | Green Silver nanoparticles Noctiluca scintillans Anti-cancer MDA-MB-231 Apoptosis Migration |
| url | http://www.sciencedirect.com/science/article/pii/S0753332223013306 |
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