Comparative Study of Structural Changes of Polylactide and Poly(ethylene terephthalate) in the Presence of <i>Trichoderma viride</i>
Plastic pollution is one of the crucial global challenges nowadays, and biodegradation is a promising approach to manage plastic waste in an environment-friendly and cost-effective way. In this study we identified the strain of fungus <i>Trichoderma viride</i> GZ1, which was characterize...
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/7/3491 |
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| author | Grażyna B. Dąbrowska Zuzanna Garstecka Ewa Olewnik-Kruszkowska Grażyna Szczepańska Maciej Ostrowski Agnieszka Mierek-Adamska |
| author_facet | Grażyna B. Dąbrowska Zuzanna Garstecka Ewa Olewnik-Kruszkowska Grażyna Szczepańska Maciej Ostrowski Agnieszka Mierek-Adamska |
| author_sort | Grażyna B. Dąbrowska |
| collection | DOAJ |
| description | Plastic pollution is one of the crucial global challenges nowadays, and biodegradation is a promising approach to manage plastic waste in an environment-friendly and cost-effective way. In this study we identified the strain of fungus <i>Trichoderma viride</i> GZ1, which was characterized by particularly high pectinolytic activity. Using differential scanning calorimetry, Fourier-transform infrared spectroscopy techniques, and viscosity measurements we showed that three-month incubation of polylactide and polyethylene terephthalate in the presence of the fungus lead to significant changes of the surface of polylactide. Further, to gain insight into molecular mechanisms underneath the biodegradation process, western blot hybridization was used to show that in the presence of poly(ethylene terephthalate) (PET) in laboratory conditions the fungus produced hydrophobin proteins. The mycelium adhered to the plastic surface, which was confirmed by scanning electron microscopy, possibly due to the presence of hydrophobins. Further, using atomic force microscopy we demonstrated for the first time the formation of hydrophobin film on the surface of aliphatic polylactide (PLA) and PET by <i>T. viride</i> GZ1. This is the first stage of research that will be continued under environmental conditions, potentially leading to a practical application. |
| first_indexed | 2024-03-10T12:50:39Z |
| format | Article |
| id | doaj.art-c1bf1d4ba2b447a0ba199b776983b7e6 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T12:50:39Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c1bf1d4ba2b447a0ba199b776983b7e62023-11-21T13:08:02ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01227349110.3390/ijms22073491Comparative Study of Structural Changes of Polylactide and Poly(ethylene terephthalate) in the Presence of <i>Trichoderma viride</i>Grażyna B. Dąbrowska0Zuzanna Garstecka1Ewa Olewnik-Kruszkowska2Grażyna Szczepańska3Maciej Ostrowski4Agnieszka Mierek-Adamska5Department of Genetics, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Toruń, PolandDepartment of Genetics, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Toruń, PolandDepartment of Physical Chemistry and Physicochemistry of Polymers, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Gagarina 7, 87-100 Toruń, PolandLaboratory for Instrumental Analysis, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Gagarina 7, 87-100 Toruń, PolandDepartment of Biochemistry, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Toruń, PolandDepartment of Genetics, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Toruń, PolandPlastic pollution is one of the crucial global challenges nowadays, and biodegradation is a promising approach to manage plastic waste in an environment-friendly and cost-effective way. In this study we identified the strain of fungus <i>Trichoderma viride</i> GZ1, which was characterized by particularly high pectinolytic activity. Using differential scanning calorimetry, Fourier-transform infrared spectroscopy techniques, and viscosity measurements we showed that three-month incubation of polylactide and polyethylene terephthalate in the presence of the fungus lead to significant changes of the surface of polylactide. Further, to gain insight into molecular mechanisms underneath the biodegradation process, western blot hybridization was used to show that in the presence of poly(ethylene terephthalate) (PET) in laboratory conditions the fungus produced hydrophobin proteins. The mycelium adhered to the plastic surface, which was confirmed by scanning electron microscopy, possibly due to the presence of hydrophobins. Further, using atomic force microscopy we demonstrated for the first time the formation of hydrophobin film on the surface of aliphatic polylactide (PLA) and PET by <i>T. viride</i> GZ1. This is the first stage of research that will be continued under environmental conditions, potentially leading to a practical application.https://www.mdpi.com/1422-0067/22/7/3491polylactidepoly(ethylene terephthalate)<i>Trichoderma viride</i>hydrophobinhydrophobin filmbioremediation |
| spellingShingle | Grażyna B. Dąbrowska Zuzanna Garstecka Ewa Olewnik-Kruszkowska Grażyna Szczepańska Maciej Ostrowski Agnieszka Mierek-Adamska Comparative Study of Structural Changes of Polylactide and Poly(ethylene terephthalate) in the Presence of <i>Trichoderma viride</i> International Journal of Molecular Sciences polylactide poly(ethylene terephthalate) <i>Trichoderma viride</i> hydrophobin hydrophobin film bioremediation |
| title | Comparative Study of Structural Changes of Polylactide and Poly(ethylene terephthalate) in the Presence of <i>Trichoderma viride</i> |
| title_full | Comparative Study of Structural Changes of Polylactide and Poly(ethylene terephthalate) in the Presence of <i>Trichoderma viride</i> |
| title_fullStr | Comparative Study of Structural Changes of Polylactide and Poly(ethylene terephthalate) in the Presence of <i>Trichoderma viride</i> |
| title_full_unstemmed | Comparative Study of Structural Changes of Polylactide and Poly(ethylene terephthalate) in the Presence of <i>Trichoderma viride</i> |
| title_short | Comparative Study of Structural Changes of Polylactide and Poly(ethylene terephthalate) in the Presence of <i>Trichoderma viride</i> |
| title_sort | comparative study of structural changes of polylactide and poly ethylene terephthalate in the presence of i trichoderma viride i |
| topic | polylactide poly(ethylene terephthalate) <i>Trichoderma viride</i> hydrophobin hydrophobin film bioremediation |
| url | https://www.mdpi.com/1422-0067/22/7/3491 |
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