Transcriptome Analysis of <i>Psacothea hilaris</i>: De Novo Assembly and Antimicrobial Peptide Prediction
Antimicrobial peptides (AMPs) are the frontline innate defense system evolutionarily preserved in insects to combat invading pathogens. These AMPs could serve as an alternative to classical antibiotics to overcome the burden of treating multidrug resistant bacteria. Psacotheasin, a knottin type AMP...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/10/676 |
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| author | Joon Ha Lee Hoyong Chung Yong Pyo Shin In-Woo Kim Sathishkumar Natarajan Karpagam Veerappan Minchul Seo Junhyung Park Jae Sam Hwang |
| author_facet | Joon Ha Lee Hoyong Chung Yong Pyo Shin In-Woo Kim Sathishkumar Natarajan Karpagam Veerappan Minchul Seo Junhyung Park Jae Sam Hwang |
| author_sort | Joon Ha Lee |
| collection | DOAJ |
| description | Antimicrobial peptides (AMPs) are the frontline innate defense system evolutionarily preserved in insects to combat invading pathogens. These AMPs could serve as an alternative to classical antibiotics to overcome the burden of treating multidrug resistant bacteria. Psacotheasin, a knottin type AMP was isolated from <i>Psacothea hilaris</i> and shown to exhibit antimicrobial activity, especially against fungi through apoptosis mediated cell death. In this study, we aimed to identify novel probable AMPs from <i>Psacothea hilaris</i>, the yellow spotted longicorn beetle. The beetle was immunized with the two bacterial strains (<i>E. coli and S. aureus</i>), and the yeast strain <i>C. albicans</i>. After immunization, total RNA was isolated and sequenced in Illumina platform. Then, beetle transcriptome was de novo assembled and searched for putative AMPs with the known physiochemical features of the AMPs. A selection of AMP candidates were synthesized and tested for antimicrobial activity. Four peptides showed stronger activity against <i>E. coli</i> than the control AMP, melittin while one peptide showed similar activity against <i>S. aureus</i>. Moreover, four peptides and two peptides showed antifungal activity stronger than and similar to melittin, respectively. Collectively one peptide showed both antibacterial and antifungal activity superior to melittin; thus, it provides a potent antimicrobial peptide. All the peptides showed no hemolysis in all the tested concentrations. These results suggest that in silico mining of insects’ transcriptome could be a promising tool to obtain and optimize novel AMPs for human needs. |
| first_indexed | 2024-03-10T15:50:07Z |
| format | Article |
| id | doaj.art-c1c1143a183f4662b1ce4e20b79af8ce |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T15:50:07Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-c1c1143a183f4662b1ce4e20b79af8ce2023-11-20T16:08:04ZengMDPI AGInsects2075-44502020-10-01111067610.3390/insects11100676Transcriptome Analysis of <i>Psacothea hilaris</i>: De Novo Assembly and Antimicrobial Peptide PredictionJoon Ha Lee0Hoyong Chung1Yong Pyo Shin2In-Woo Kim3Sathishkumar Natarajan4Karpagam Veerappan5Minchul Seo6Junhyung Park7Jae Sam Hwang8Department of Agricultural Biology, National Institute of Agricultural Sciences, 166, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 55365, Korea3BIGS CO. LTD., 156, Gwanggyo-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do 16506, KoreaDepartment of Agricultural Biology, National Institute of Agricultural Sciences, 166, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 55365, KoreaDepartment of Agricultural Biology, National Institute of Agricultural Sciences, 166, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 55365, Korea3BIGS CO. LTD., 156, Gwanggyo-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do 16506, Korea3BIGS CO. LTD., 156, Gwanggyo-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do 16506, KoreaDepartment of Agricultural Biology, National Institute of Agricultural Sciences, 166, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 55365, Korea3BIGS CO. LTD., 156, Gwanggyo-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do 16506, KoreaDepartment of Agricultural Biology, National Institute of Agricultural Sciences, 166, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 55365, KoreaAntimicrobial peptides (AMPs) are the frontline innate defense system evolutionarily preserved in insects to combat invading pathogens. These AMPs could serve as an alternative to classical antibiotics to overcome the burden of treating multidrug resistant bacteria. Psacotheasin, a knottin type AMP was isolated from <i>Psacothea hilaris</i> and shown to exhibit antimicrobial activity, especially against fungi through apoptosis mediated cell death. In this study, we aimed to identify novel probable AMPs from <i>Psacothea hilaris</i>, the yellow spotted longicorn beetle. The beetle was immunized with the two bacterial strains (<i>E. coli and S. aureus</i>), and the yeast strain <i>C. albicans</i>. After immunization, total RNA was isolated and sequenced in Illumina platform. Then, beetle transcriptome was de novo assembled and searched for putative AMPs with the known physiochemical features of the AMPs. A selection of AMP candidates were synthesized and tested for antimicrobial activity. Four peptides showed stronger activity against <i>E. coli</i> than the control AMP, melittin while one peptide showed similar activity against <i>S. aureus</i>. Moreover, four peptides and two peptides showed antifungal activity stronger than and similar to melittin, respectively. Collectively one peptide showed both antibacterial and antifungal activity superior to melittin; thus, it provides a potent antimicrobial peptide. All the peptides showed no hemolysis in all the tested concentrations. These results suggest that in silico mining of insects’ transcriptome could be a promising tool to obtain and optimize novel AMPs for human needs.https://www.mdpi.com/2075-4450/11/10/676<i>Psacothea hilaris</i>knottinpsacotheasinantimicrobial peptidetranscriptomehemolysis |
| spellingShingle | Joon Ha Lee Hoyong Chung Yong Pyo Shin In-Woo Kim Sathishkumar Natarajan Karpagam Veerappan Minchul Seo Junhyung Park Jae Sam Hwang Transcriptome Analysis of <i>Psacothea hilaris</i>: De Novo Assembly and Antimicrobial Peptide Prediction Insects <i>Psacothea hilaris</i> knottin psacotheasin antimicrobial peptide transcriptome hemolysis |
| title | Transcriptome Analysis of <i>Psacothea hilaris</i>: De Novo Assembly and Antimicrobial Peptide Prediction |
| title_full | Transcriptome Analysis of <i>Psacothea hilaris</i>: De Novo Assembly and Antimicrobial Peptide Prediction |
| title_fullStr | Transcriptome Analysis of <i>Psacothea hilaris</i>: De Novo Assembly and Antimicrobial Peptide Prediction |
| title_full_unstemmed | Transcriptome Analysis of <i>Psacothea hilaris</i>: De Novo Assembly and Antimicrobial Peptide Prediction |
| title_short | Transcriptome Analysis of <i>Psacothea hilaris</i>: De Novo Assembly and Antimicrobial Peptide Prediction |
| title_sort | transcriptome analysis of i psacothea hilaris i de novo assembly and antimicrobial peptide prediction |
| topic | <i>Psacothea hilaris</i> knottin psacotheasin antimicrobial peptide transcriptome hemolysis |
| url | https://www.mdpi.com/2075-4450/11/10/676 |
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