Targeted Mutagenesis of NAC Transcription Factor Gene, OsNAC041, Leading to Salt Sensitivity in Rice
Salinity is a major abiotic stress factor that seriously affects plant growth. Many genes are involved in the response to salt stress with various metabolism pathways. A number of plant transcription factor family genes have been found to be involved in the salt stress response, and NAM, ATAF and CU...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-03-01
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| Series: | Rice Science |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1672630819300046 |
| _version_ | 1819226230838263808 |
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| author | Wang Bo Zhong Zhaohui Zhang Huanhuan Wang Xia Liu Binglin Yang Lijia Han Xiangyan Yu Deshui Zheng Xuelian Wang Chunguo Song Wenqin Chen Chengbin Zhang Yong |
| author_facet | Wang Bo Zhong Zhaohui Zhang Huanhuan Wang Xia Liu Binglin Yang Lijia Han Xiangyan Yu Deshui Zheng Xuelian Wang Chunguo Song Wenqin Chen Chengbin Zhang Yong |
| author_sort | Wang Bo |
| collection | DOAJ |
| description | Salinity is a major abiotic stress factor that seriously affects plant growth. Many genes are involved in the response to salt stress with various metabolism pathways. A number of plant transcription factor family genes have been found to be involved in the salt stress response, and NAM, ATAF and CUC (NAC) transcription factors are thought to act as active regulators during abiotic stress, especially salt stress. In this study, we detected a rice NAC transcription factor coding gene, OsNAC041, and confirmed that it influenced the germination of seeds under salt stress and salt tolerance of plants. OsNAC041 was primarily expressed in the leaves and located in the nucleus. Furthermore, the CRISPR/Cas9 method was used to obtain a targeted osnac041 mutant, of which the plant height was higher than that of the wild-type, showing increased salt sensitivity. Moreover, RNA-seq analysis revealed a number of differentially expressed genes (DEGs) involved in several important signaling pathways in the osnac041 mutant. Subsequently, Kyoto Encyclopedia of Genes and Genomes annotation also revealed differential expression of DEGs associated with mitogen-activated protein kinase signaling, peroxisome, eukaryotic- type ABC transporters, photosynthesis and plant hormones, which are involved in stress-related signaling pathways. Overall, our study suggested that OsNAC041 was involved in the salt stress response in rice. These findings not only provide empirical evidence of OsNAC041 function, but also provide new insight into its potential application in rice resistance breeding. Keywords: rice, NAC transcription factor, CRISPR, Cas9, RNA-seq, salinity |
| first_indexed | 2024-12-23T10:22:12Z |
| format | Article |
| id | doaj.art-c1c39f6893564a76abdc4793f39af153 |
| institution | Directory Open Access Journal |
| issn | 1672-6308 |
| language | English |
| last_indexed | 2024-12-23T10:22:12Z |
| publishDate | 2019-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Rice Science |
| spelling | doaj.art-c1c39f6893564a76abdc4793f39af1532022-12-21T17:50:39ZengElsevierRice Science1672-63082019-03-0126298108Targeted Mutagenesis of NAC Transcription Factor Gene, OsNAC041, Leading to Salt Sensitivity in RiceWang Bo0Zhong Zhaohui1Zhang Huanhuan2Wang Xia3Liu Binglin4Yang Lijia5Han Xiangyan6Yu Deshui7Zheng Xuelian8Wang Chunguo9Song Wenqin10Chen Chengbin11Zhang Yong12College of Life Sciences, Nankai University, Tianjin 300071, China; Department of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, ChinaDepartment of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, ChinaCollege of Life Sciences, Nankai University, Tianjin 300071, ChinaCollege of Life Sciences, Nankai University, Tianjin 300071, ChinaDepartment of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, ChinaDepartment of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, ChinaCollege of Life Sciences, Nankai University, Tianjin 300071, ChinaCollege of Life Sciences, Nankai University, Tianjin 300071, ChinaDepartment of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, ChinaCollege of Life Sciences, Nankai University, Tianjin 300071, ChinaCollege of Life Sciences, Nankai University, Tianjin 300071, ChinaCollege of Life Sciences, Nankai University, Tianjin 300071, China; Corresponding authors.Department of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, China; Corresponding authors.Salinity is a major abiotic stress factor that seriously affects plant growth. Many genes are involved in the response to salt stress with various metabolism pathways. A number of plant transcription factor family genes have been found to be involved in the salt stress response, and NAM, ATAF and CUC (NAC) transcription factors are thought to act as active regulators during abiotic stress, especially salt stress. In this study, we detected a rice NAC transcription factor coding gene, OsNAC041, and confirmed that it influenced the germination of seeds under salt stress and salt tolerance of plants. OsNAC041 was primarily expressed in the leaves and located in the nucleus. Furthermore, the CRISPR/Cas9 method was used to obtain a targeted osnac041 mutant, of which the plant height was higher than that of the wild-type, showing increased salt sensitivity. Moreover, RNA-seq analysis revealed a number of differentially expressed genes (DEGs) involved in several important signaling pathways in the osnac041 mutant. Subsequently, Kyoto Encyclopedia of Genes and Genomes annotation also revealed differential expression of DEGs associated with mitogen-activated protein kinase signaling, peroxisome, eukaryotic- type ABC transporters, photosynthesis and plant hormones, which are involved in stress-related signaling pathways. Overall, our study suggested that OsNAC041 was involved in the salt stress response in rice. These findings not only provide empirical evidence of OsNAC041 function, but also provide new insight into its potential application in rice resistance breeding. Keywords: rice, NAC transcription factor, CRISPR, Cas9, RNA-seq, salinityhttp://www.sciencedirect.com/science/article/pii/S1672630819300046 |
| spellingShingle | Wang Bo Zhong Zhaohui Zhang Huanhuan Wang Xia Liu Binglin Yang Lijia Han Xiangyan Yu Deshui Zheng Xuelian Wang Chunguo Song Wenqin Chen Chengbin Zhang Yong Targeted Mutagenesis of NAC Transcription Factor Gene, OsNAC041, Leading to Salt Sensitivity in Rice Rice Science |
| title | Targeted Mutagenesis of NAC Transcription Factor Gene, OsNAC041, Leading to Salt Sensitivity in Rice |
| title_full | Targeted Mutagenesis of NAC Transcription Factor Gene, OsNAC041, Leading to Salt Sensitivity in Rice |
| title_fullStr | Targeted Mutagenesis of NAC Transcription Factor Gene, OsNAC041, Leading to Salt Sensitivity in Rice |
| title_full_unstemmed | Targeted Mutagenesis of NAC Transcription Factor Gene, OsNAC041, Leading to Salt Sensitivity in Rice |
| title_short | Targeted Mutagenesis of NAC Transcription Factor Gene, OsNAC041, Leading to Salt Sensitivity in Rice |
| title_sort | targeted mutagenesis of nac transcription factor gene osnac041 leading to salt sensitivity in rice |
| url | http://www.sciencedirect.com/science/article/pii/S1672630819300046 |
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