Characterization of Phytophthora nicotianae isolates from tobacco plants (Nicotiana tabacum) in Colombia
ABSTRACT The black shank disease caused by Phytophthora nicotianae causes losses in tobacco crops up 100%. In Colombia, P. nicotianae populations are poorly known causing wrong diagnostics and erratic management. Amplification of the Ypt1 gene and morphological characteristics of colonies, sporangia...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal De Viçosa
2022-01-01
|
| Series: | Revista Ceres |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2022000100078&lng=en&tlng=en |
| _version_ | 1798013810554961920 |
|---|---|
| author | Diana Marcela Vanegas Rafael Angel Navarro Lucia Alfanador Jaime Andres Gutierrez Juan Gonzalo Morales Bertha Miryam Gaviria |
| author_facet | Diana Marcela Vanegas Rafael Angel Navarro Lucia Alfanador Jaime Andres Gutierrez Juan Gonzalo Morales Bertha Miryam Gaviria |
| author_sort | Diana Marcela Vanegas |
| collection | DOAJ |
| description | ABSTRACT The black shank disease caused by Phytophthora nicotianae causes losses in tobacco crops up 100%. In Colombia, P. nicotianae populations are poorly known causing wrong diagnostics and erratic management. Amplification of the Ypt1 gene and morphological characteristics of colonies, sporangia, chlamydospores and hyphae were used to identify P. nicotianae isolates. Races were identified according to the reaction induced by each isolate on the differential tobacco varieties Hicks, L8, KY 14 x L8 and NC 1071. As results, 71 isolates of P. nicotianae were identified and classified by races. Colonies of P. nicotianae were of white color, cottony and fluffy texture with smooth, non-swollen hyphae; spherical papillae with an average of 1.26 μm and non-papillated and intercalary chlamydospores of medium size of 1.02 μm that are typical characteristics of P. nicotianae. A species-specific PCR-amplified band of 389 bp was detected in all isolates tested. The presence of races 0, 1 and 3 of P. nicotianae were determined in the Colombian departments of Huila and Santander. To the best of our knowledge, this is the first report of physiological races 0, 1 and 3 of P. nicotianae in Colombia. Results are of relevance for disease management and tobacco breeding. |
| first_indexed | 2024-04-11T15:07:29Z |
| format | Article |
| id | doaj.art-c1c54f743eaa48f18e7b8ff60fad5299 |
| institution | Directory Open Access Journal |
| issn | 2177-3491 |
| language | English |
| last_indexed | 2024-04-11T15:07:29Z |
| publishDate | 2022-01-01 |
| publisher | Universidade Federal De Viçosa |
| record_format | Article |
| series | Revista Ceres |
| spelling | doaj.art-c1c54f743eaa48f18e7b8ff60fad52992022-12-22T04:16:45ZengUniversidade Federal De ViçosaRevista Ceres2177-34912022-01-01691789110.1590/0034-737x202269010011Characterization of Phytophthora nicotianae isolates from tobacco plants (Nicotiana tabacum) in ColombiaDiana Marcela Vanegashttps://orcid.org/0000-0002-9504-1950Rafael Angel NavarroLucia AlfanadorJaime Andres GutierrezJuan Gonzalo MoralesBertha Miryam GaviriaABSTRACT The black shank disease caused by Phytophthora nicotianae causes losses in tobacco crops up 100%. In Colombia, P. nicotianae populations are poorly known causing wrong diagnostics and erratic management. Amplification of the Ypt1 gene and morphological characteristics of colonies, sporangia, chlamydospores and hyphae were used to identify P. nicotianae isolates. Races were identified according to the reaction induced by each isolate on the differential tobacco varieties Hicks, L8, KY 14 x L8 and NC 1071. As results, 71 isolates of P. nicotianae were identified and classified by races. Colonies of P. nicotianae were of white color, cottony and fluffy texture with smooth, non-swollen hyphae; spherical papillae with an average of 1.26 μm and non-papillated and intercalary chlamydospores of medium size of 1.02 μm that are typical characteristics of P. nicotianae. A species-specific PCR-amplified band of 389 bp was detected in all isolates tested. The presence of races 0, 1 and 3 of P. nicotianae were determined in the Colombian departments of Huila and Santander. To the best of our knowledge, this is the first report of physiological races 0, 1 and 3 of P. nicotianae in Colombia. Results are of relevance for disease management and tobacco breeding.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2022000100078&lng=en&tlng=enchlamydosporesblack shankoomycetesraces 0, 1, 3sporangiaYpT1 gene. |
| spellingShingle | Diana Marcela Vanegas Rafael Angel Navarro Lucia Alfanador Jaime Andres Gutierrez Juan Gonzalo Morales Bertha Miryam Gaviria Characterization of Phytophthora nicotianae isolates from tobacco plants (Nicotiana tabacum) in Colombia Revista Ceres chlamydospores black shank oomycetes races 0, 1, 3 sporangia YpT1 gene. |
| title | Characterization of Phytophthora nicotianae isolates from tobacco plants (Nicotiana tabacum) in Colombia |
| title_full | Characterization of Phytophthora nicotianae isolates from tobacco plants (Nicotiana tabacum) in Colombia |
| title_fullStr | Characterization of Phytophthora nicotianae isolates from tobacco plants (Nicotiana tabacum) in Colombia |
| title_full_unstemmed | Characterization of Phytophthora nicotianae isolates from tobacco plants (Nicotiana tabacum) in Colombia |
| title_short | Characterization of Phytophthora nicotianae isolates from tobacco plants (Nicotiana tabacum) in Colombia |
| title_sort | characterization of phytophthora nicotianae isolates from tobacco plants nicotiana tabacum in colombia |
| topic | chlamydospores black shank oomycetes races 0, 1, 3 sporangia YpT1 gene. |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2022000100078&lng=en&tlng=en |
| work_keys_str_mv | AT dianamarcelavanegas characterizationofphytophthoranicotianaeisolatesfromtobaccoplantsnicotianatabacumincolombia AT rafaelangelnavarro characterizationofphytophthoranicotianaeisolatesfromtobaccoplantsnicotianatabacumincolombia AT luciaalfanador characterizationofphytophthoranicotianaeisolatesfromtobaccoplantsnicotianatabacumincolombia AT jaimeandresgutierrez characterizationofphytophthoranicotianaeisolatesfromtobaccoplantsnicotianatabacumincolombia AT juangonzalomorales characterizationofphytophthoranicotianaeisolatesfromtobaccoplantsnicotianatabacumincolombia AT berthamiryamgaviria characterizationofphytophthoranicotianaeisolatesfromtobaccoplantsnicotianatabacumincolombia |