Genome sequencing of drake semen micobiome with correlation with their compositions, sources and potential mechanisms affecting semen quality
ABSTRACT: Artificial insemination (AI) technology has greatly promoted the development of the chicken industry. Recently, AI technology has also begun to be used in the duck industry, but there are some problems. Numerous researchers have shown that microbes colonizing in semen can degrade semen qua...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124001123 |
| _version_ | 1797256505068617728 |
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| author | Xinyue Hu Jie Li Shuai Xin Qingyuan Ouyang Jialu Li Lipeng Zhu Jiwei Hu Hua He Hehe Liu Liang Li Shenqiang Hu Jiwen Wang |
| author_facet | Xinyue Hu Jie Li Shuai Xin Qingyuan Ouyang Jialu Li Lipeng Zhu Jiwei Hu Hua He Hehe Liu Liang Li Shenqiang Hu Jiwen Wang |
| author_sort | Xinyue Hu |
| collection | DOAJ |
| description | ABSTRACT: Artificial insemination (AI) technology has greatly promoted the development of the chicken industry. Recently, AI technology has also begun to be used in the duck industry, but there are some problems. Numerous researchers have shown that microbes colonizing in semen can degrade semen quality, and AI can increase the harmful microbial load in hen's reproductive tract. Different from the degraded external genitalia of roosters, drakes have well-developed external genitalia, which may cause drake semen to be more susceptible to microbial contamination. However, information on the compositions, sources, and effects of semen microbes on semen quality remains unknown in drakes. In the current study, high-throughput sequencing technology was used to detect microbial communities in drake semen, environmental swabs, cloacal swabs, and the spermaduct after quantifying the semen quality of drakes to investigate the effects of microbes in the environment, cloaca, and spermaduct on semen microbiota and the relationships between semen microbes and semen quality. Taxonomic analysis showed that the microbes in the semen, environment, cloaca, and spermaduct samples were all classified into 4 phyla and 25 genera. Firmicutes and Proteobacteria were the dominant phyla. Phyllobacterium only existed in the environment, while Marinococcus did not exist in the cloaca. Of the 24 genera present in semen: Brachybacterium, Brochothrix, Chryseobacterium, Kocuria, Marinococcus, Micrococcus, Rothia, Salinicoccus, and Staphylococcus originated from the environment; Achromobacter, Aerococcus, Corynebacterium, Desemzia, Enterococcus, Jeotgalicoccus, Pseudomonas, Psychrobacter, and Turicibacter originated from the cloaca; and Agrobacterium, Carnobacterium, Chelativorans, Devosia, Halomonas, and Oceanicaulis originated from the spermaduct. In addition, K-means clustering analysis showed that semen samples could be divided into 2 clusters based on microbial compositions, and compared with cluster 1, the counts of Chelativorans (P < 0.05), Devosia (P < 0.01), Halomonas (P < 0.05), and Oceanicaulis (P < 0.05) were higher in cluster 2, while the sperm viability (P < 0.05), total sperm number (P < 0.01), and semen quality factor (SQF) (P < 0.01) were lower in cluster 2. Furthermore, functional prediction analysis of microbes showed that the activities of starch and sucrose metabolism, phosphotransferase system, ABC transporters, microbial metabolism in diverse environments, and quorum sensing pathways between cluster 1 and cluster 2 were significantly different (P < 0.05). Overall, environmental/cloacal microbes resulted in semen contamination, and microbes from the Chelativorans, Devosia, Halomonas, and Oceanicaulis genera may have negative effects on semen quality in drakes by affecting the activities of starch and sucrose metabolism, phosphotransferase system, ABC transporters, and quorum sensing pathways that are associated with carbohydrate metabolism. These data will provide a basis for developing strategies to prevent microbial contamination of drake semen. |
| first_indexed | 2024-03-08T00:24:11Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-24T22:22:48Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
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| series | Poultry Science |
| spelling | doaj.art-c1c8dffa8c71462e8f739c63183848222024-03-20T06:08:00ZengElsevierPoultry Science0032-57912024-04-011034103533Genome sequencing of drake semen micobiome with correlation with their compositions, sources and potential mechanisms affecting semen qualityXinyue Hu0Jie Li1Shuai Xin2Qingyuan Ouyang3Jialu Li4Lipeng Zhu5Jiwei Hu6Hua He7Hehe Liu8Liang Li9Shenqiang Hu10Jiwen Wang11Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Chengdu Campus, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Chengdu Campus, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Chengdu Campus, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Chengdu Campus, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Chengdu Campus, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Chengdu Campus, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Chengdu Campus, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Chengdu Campus, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Chengdu Campus, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Chengdu Campus, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Chengdu Campus, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaCorrespondence author:; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Chengdu Campus, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaABSTRACT: Artificial insemination (AI) technology has greatly promoted the development of the chicken industry. Recently, AI technology has also begun to be used in the duck industry, but there are some problems. Numerous researchers have shown that microbes colonizing in semen can degrade semen quality, and AI can increase the harmful microbial load in hen's reproductive tract. Different from the degraded external genitalia of roosters, drakes have well-developed external genitalia, which may cause drake semen to be more susceptible to microbial contamination. However, information on the compositions, sources, and effects of semen microbes on semen quality remains unknown in drakes. In the current study, high-throughput sequencing technology was used to detect microbial communities in drake semen, environmental swabs, cloacal swabs, and the spermaduct after quantifying the semen quality of drakes to investigate the effects of microbes in the environment, cloaca, and spermaduct on semen microbiota and the relationships between semen microbes and semen quality. Taxonomic analysis showed that the microbes in the semen, environment, cloaca, and spermaduct samples were all classified into 4 phyla and 25 genera. Firmicutes and Proteobacteria were the dominant phyla. Phyllobacterium only existed in the environment, while Marinococcus did not exist in the cloaca. Of the 24 genera present in semen: Brachybacterium, Brochothrix, Chryseobacterium, Kocuria, Marinococcus, Micrococcus, Rothia, Salinicoccus, and Staphylococcus originated from the environment; Achromobacter, Aerococcus, Corynebacterium, Desemzia, Enterococcus, Jeotgalicoccus, Pseudomonas, Psychrobacter, and Turicibacter originated from the cloaca; and Agrobacterium, Carnobacterium, Chelativorans, Devosia, Halomonas, and Oceanicaulis originated from the spermaduct. In addition, K-means clustering analysis showed that semen samples could be divided into 2 clusters based on microbial compositions, and compared with cluster 1, the counts of Chelativorans (P < 0.05), Devosia (P < 0.01), Halomonas (P < 0.05), and Oceanicaulis (P < 0.05) were higher in cluster 2, while the sperm viability (P < 0.05), total sperm number (P < 0.01), and semen quality factor (SQF) (P < 0.01) were lower in cluster 2. Furthermore, functional prediction analysis of microbes showed that the activities of starch and sucrose metabolism, phosphotransferase system, ABC transporters, microbial metabolism in diverse environments, and quorum sensing pathways between cluster 1 and cluster 2 were significantly different (P < 0.05). Overall, environmental/cloacal microbes resulted in semen contamination, and microbes from the Chelativorans, Devosia, Halomonas, and Oceanicaulis genera may have negative effects on semen quality in drakes by affecting the activities of starch and sucrose metabolism, phosphotransferase system, ABC transporters, and quorum sensing pathways that are associated with carbohydrate metabolism. These data will provide a basis for developing strategies to prevent microbial contamination of drake semen.http://www.sciencedirect.com/science/article/pii/S003257912400112316SrRNA sequencingartificial inseminationCloacaenvironmentspermaduct |
| spellingShingle | Xinyue Hu Jie Li Shuai Xin Qingyuan Ouyang Jialu Li Lipeng Zhu Jiwei Hu Hua He Hehe Liu Liang Li Shenqiang Hu Jiwen Wang Genome sequencing of drake semen micobiome with correlation with their compositions, sources and potential mechanisms affecting semen quality Poultry Science 16SrRNA sequencing artificial insemination Cloaca environment spermaduct |
| title | Genome sequencing of drake semen micobiome with correlation with their compositions, sources and potential mechanisms affecting semen quality |
| title_full | Genome sequencing of drake semen micobiome with correlation with their compositions, sources and potential mechanisms affecting semen quality |
| title_fullStr | Genome sequencing of drake semen micobiome with correlation with their compositions, sources and potential mechanisms affecting semen quality |
| title_full_unstemmed | Genome sequencing of drake semen micobiome with correlation with their compositions, sources and potential mechanisms affecting semen quality |
| title_short | Genome sequencing of drake semen micobiome with correlation with their compositions, sources and potential mechanisms affecting semen quality |
| title_sort | genome sequencing of drake semen micobiome with correlation with their compositions sources and potential mechanisms affecting semen quality |
| topic | 16SrRNA sequencing artificial insemination Cloaca environment spermaduct |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124001123 |
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