The Effects of Exercise Training on Vascular Endothelial Growth Factor and Endostatin Gene Expression in Cardiac Tissue of Rats with Type 2 Diabetes Mellitus
Background: Impaired angiogenesis and formation of collateral vessels in skeletal and cardiac muscle, is a major complication of type 2 diabetes mellitus in ischemia. The aim of this study was to evaluate the changes in vascular endothelial grows factor (VEGF) and Endostatin gene expression in cardi...
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Isfahan University of Medical Sciences
2018-10-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/10214 |
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| author | Saeed Esmaeili Vazgen Minasian Hadi Karamii Mohamad Bayat |
| author_facet | Saeed Esmaeili Vazgen Minasian Hadi Karamii Mohamad Bayat |
| author_sort | Saeed Esmaeili |
| collection | DOAJ |
| description | Background: Impaired angiogenesis and formation of collateral vessels in skeletal and cardiac muscle, is a major complication of type 2 diabetes mellitus in ischemia. The aim of this study was to evaluate the changes in vascular endothelial grows factor (VEGF) and Endostatin gene expression in cardiac tissue of rats with type 2 diabetes mellitus following aerobic continues and high-intensity interval exercise training.
Methods: In this study, 48 Wistar rats were randomly divided into 4 equal groups of healthy control, diabetic control, diabetic continues, and high-intensity interval training. Type 2 diabetes mellitus was induced by intra-peritoneal injection of streptozotocin, and exercise performed 5 sessions per week for 8 weeks. The levels of VEGF and Endostatin gene expression were evaluated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) method.
Findings: There was a significant reduction in the expression of VEGF and Endostatin in the diabetic control group compared to the healthy control group. Compared to the diabetic control group, diabetic continues and high-intensity interval training groups showed a significant increase in expression of VEGF, but there was significant increase in Endostatin expression in diabetic continues group.
Conclusion: The results of this study showed that both training methods were effective to increase the expression of VEGF and Endostatin levels after induction of type 2 diabetes mellitus. Since, there was increase in VEGF/Endostatin ratio, increase in pro-angiogenic activity in heart may be occurred, but the high-intensity interval exercise training was more effective to increase the expression of VEGF. |
| first_indexed | 2024-03-12T09:00:42Z |
| format | Article |
| id | doaj.art-c1c9494693c74bd890139c36d32ff772 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T09:00:42Z |
| publishDate | 2018-10-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-c1c9494693c74bd890139c36d32ff7722023-09-02T15:43:27ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2018-10-013649190991610.22122/jims.v36i491.102143157The Effects of Exercise Training on Vascular Endothelial Growth Factor and Endostatin Gene Expression in Cardiac Tissue of Rats with Type 2 Diabetes MellitusSaeed Esmaeili0Vazgen Minasian1Hadi Karamii2Mohamad Bayat3PhD Candidate, Department of Exercise Physiology, School of Sport Sciences, University of Isfahan, Isfahan, IranAssociate Professor, Department of Exercise Physiology, School of Sport Sciences, University of Isfahan, Isfahan, IranAssistant Professor, Department of Molecular Medicine and Biotechnology, School of Medicine, Arak University of Medical Sciences, Arak, IranAssistant Professor, Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, IranBackground: Impaired angiogenesis and formation of collateral vessels in skeletal and cardiac muscle, is a major complication of type 2 diabetes mellitus in ischemia. The aim of this study was to evaluate the changes in vascular endothelial grows factor (VEGF) and Endostatin gene expression in cardiac tissue of rats with type 2 diabetes mellitus following aerobic continues and high-intensity interval exercise training. Methods: In this study, 48 Wistar rats were randomly divided into 4 equal groups of healthy control, diabetic control, diabetic continues, and high-intensity interval training. Type 2 diabetes mellitus was induced by intra-peritoneal injection of streptozotocin, and exercise performed 5 sessions per week for 8 weeks. The levels of VEGF and Endostatin gene expression were evaluated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) method. Findings: There was a significant reduction in the expression of VEGF and Endostatin in the diabetic control group compared to the healthy control group. Compared to the diabetic control group, diabetic continues and high-intensity interval training groups showed a significant increase in expression of VEGF, but there was significant increase in Endostatin expression in diabetic continues group. Conclusion: The results of this study showed that both training methods were effective to increase the expression of VEGF and Endostatin levels after induction of type 2 diabetes mellitus. Since, there was increase in VEGF/Endostatin ratio, increase in pro-angiogenic activity in heart may be occurred, but the high-intensity interval exercise training was more effective to increase the expression of VEGF.http://jims.mui.ac.ir/index.php/jims/article/view/10214Type 2 diabetes mellitusVascular endothelial growth factorEndostatinsHigh-intensity interval training |
| spellingShingle | Saeed Esmaeili Vazgen Minasian Hadi Karamii Mohamad Bayat The Effects of Exercise Training on Vascular Endothelial Growth Factor and Endostatin Gene Expression in Cardiac Tissue of Rats with Type 2 Diabetes Mellitus مجله دانشکده پزشکی اصفهان Type 2 diabetes mellitus Vascular endothelial growth factor Endostatins High-intensity interval training |
| title | The Effects of Exercise Training on Vascular Endothelial Growth Factor and Endostatin Gene Expression in Cardiac Tissue of Rats with Type 2 Diabetes Mellitus |
| title_full | The Effects of Exercise Training on Vascular Endothelial Growth Factor and Endostatin Gene Expression in Cardiac Tissue of Rats with Type 2 Diabetes Mellitus |
| title_fullStr | The Effects of Exercise Training on Vascular Endothelial Growth Factor and Endostatin Gene Expression in Cardiac Tissue of Rats with Type 2 Diabetes Mellitus |
| title_full_unstemmed | The Effects of Exercise Training on Vascular Endothelial Growth Factor and Endostatin Gene Expression in Cardiac Tissue of Rats with Type 2 Diabetes Mellitus |
| title_short | The Effects of Exercise Training on Vascular Endothelial Growth Factor and Endostatin Gene Expression in Cardiac Tissue of Rats with Type 2 Diabetes Mellitus |
| title_sort | effects of exercise training on vascular endothelial growth factor and endostatin gene expression in cardiac tissue of rats with type 2 diabetes mellitus |
| topic | Type 2 diabetes mellitus Vascular endothelial growth factor Endostatins High-intensity interval training |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/10214 |
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