Role of Hemin in the Immune Response of T Follicular Helper Lymphocytes Expressing T-Cell Immunoreceptor with Immunoglobulin and Immunoreceptor Tyrosine-Based Inhibitory Domains, Programmed Cell Death-1, and Interleukin-21 in Allo-Auto Positive and Negative Thalassemia
Betty Agustina Tambunan,1– 3 I Dewa Gede Ugrasena,3,4 Aryati2,3 1Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; 2Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; 3Dr. Soetomo General Academic Hos...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-01-01
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| Series: | Journal of Blood Medicine |
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| Online Access: | https://www.dovepress.com/role-of-hemin-in-the-immune-response-of-t-follicular-helper-lymphocyte-peer-reviewed-fulltext-article-JBM |
| _version_ | 1797954397232168960 |
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| author | Tambunan BA Ugrasena IDG Aryati |
| author_facet | Tambunan BA Ugrasena IDG Aryati |
| author_sort | Tambunan BA |
| collection | DOAJ |
| description | Betty Agustina Tambunan,1– 3 I Dewa Gede Ugrasena,3,4 Aryati2,3 1Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; 2Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; 3Dr. Soetomo General Academic Hospital, Surabaya, Indonesia; 4Department of Pediatrics, Faculty of Medicine, Universitas Airlangga, Surabaya, IndonesiaCorrespondence: Aryati, Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, St. Mayjen. Prof. Dr. Moestopo 47, Surabaya, East Java, 60132, Indonesia, Tel +6281230570493 ; +62-031-5020251, Email aryati@fk.unair.ac.idIntroduction: Repeated transfusions in thalassemia patients can cause several complications, including alloimmunization and autoimmunization.Purpose: This study compares the immune response of T follicular helper (Tfh) lymphocytes expressing T-cell immunoreceptor with immunoglobulin and immunoreceptor tyrosine-based inhibitory domains (TIGIT), programmed cell death-1 (PD-1), and interleukin-21 (IL-21) between patients with allo-auto positive and negative thalassemia before and after hemin administration.Materials and Methods: This study used a quasi-experimental pre- and post-test design and was performed between April and November 2021 at the Dr. Soetomo General Academic Hospital in Surabaya, Indonesia. It enrolled 29 patients with allo-auto positive thalassemia and 28 with allo-auto negative, and 9 mL of whole blood (WB) was drawn from each patient. Hemin solution (20 μM) was added to 5 mL of WB, incubated for two hours, processed into peripheral blood mononuclear cells (PBMCs) in RPMI media, and cultured with 5% CO2 for three days. The 4 mL WB sample was also processed into PBMCs. PBMC cells cultured and without cultured were examined by flow cytometry using a BD FACSCalibur after surface and intracellular staining. Differences in Tfh cells expressing TIGIT, PD-1, and IL-21 between thalassemia groups before and after hemin administration were compared using independent t-tests or Mann–Whitney U-tests (p < 0.05).Results: Tfh cell expression did not differ between groups before hemin administration and increased after hemin administration. The increase in Tfh cell expression was higher in the allo-auto positive group. TIGIT and PD-1 expression in Tfh cells did not differ between groups, but TIGIT decreased after hemin administration in contrast to PD-1 result. IL-21 expression in Tfh cells did not differ between groups and did not change after hemin administration.Conclusion: Hemin affected the expression of Tfh cells in both group thalassemia, but there was no difference of Tfh cell expression between the groups.Keywords: thalassemia disease, T follicular helper, TIGIT, PD-1, interleukin-21 |
| first_indexed | 2024-04-10T23:18:00Z |
| format | Article |
| id | doaj.art-c1c961582fe047b2b34a73e97398c560 |
| institution | Directory Open Access Journal |
| issn | 1179-2736 |
| language | English |
| last_indexed | 2024-04-10T23:18:00Z |
| publishDate | 2023-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Blood Medicine |
| spelling | doaj.art-c1c961582fe047b2b34a73e97398c5602023-01-12T18:03:06ZengDove Medical PressJournal of Blood Medicine1179-27362023-01-01Volume 1471780970Role of Hemin in the Immune Response of T Follicular Helper Lymphocytes Expressing T-Cell Immunoreceptor with Immunoglobulin and Immunoreceptor Tyrosine-Based Inhibitory Domains, Programmed Cell Death-1, and Interleukin-21 in Allo-Auto Positive and Negative ThalassemiaTambunan BAUgrasena IDGAryatiBetty Agustina Tambunan,1– 3 I Dewa Gede Ugrasena,3,4 Aryati2,3 1Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; 2Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; 3Dr. Soetomo General Academic Hospital, Surabaya, Indonesia; 4Department of Pediatrics, Faculty of Medicine, Universitas Airlangga, Surabaya, IndonesiaCorrespondence: Aryati, Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, St. Mayjen. Prof. Dr. Moestopo 47, Surabaya, East Java, 60132, Indonesia, Tel +6281230570493 ; +62-031-5020251, Email aryati@fk.unair.ac.idIntroduction: Repeated transfusions in thalassemia patients can cause several complications, including alloimmunization and autoimmunization.Purpose: This study compares the immune response of T follicular helper (Tfh) lymphocytes expressing T-cell immunoreceptor with immunoglobulin and immunoreceptor tyrosine-based inhibitory domains (TIGIT), programmed cell death-1 (PD-1), and interleukin-21 (IL-21) between patients with allo-auto positive and negative thalassemia before and after hemin administration.Materials and Methods: This study used a quasi-experimental pre- and post-test design and was performed between April and November 2021 at the Dr. Soetomo General Academic Hospital in Surabaya, Indonesia. It enrolled 29 patients with allo-auto positive thalassemia and 28 with allo-auto negative, and 9 mL of whole blood (WB) was drawn from each patient. Hemin solution (20 μM) was added to 5 mL of WB, incubated for two hours, processed into peripheral blood mononuclear cells (PBMCs) in RPMI media, and cultured with 5% CO2 for three days. The 4 mL WB sample was also processed into PBMCs. PBMC cells cultured and without cultured were examined by flow cytometry using a BD FACSCalibur after surface and intracellular staining. Differences in Tfh cells expressing TIGIT, PD-1, and IL-21 between thalassemia groups before and after hemin administration were compared using independent t-tests or Mann–Whitney U-tests (p < 0.05).Results: Tfh cell expression did not differ between groups before hemin administration and increased after hemin administration. The increase in Tfh cell expression was higher in the allo-auto positive group. TIGIT and PD-1 expression in Tfh cells did not differ between groups, but TIGIT decreased after hemin administration in contrast to PD-1 result. IL-21 expression in Tfh cells did not differ between groups and did not change after hemin administration.Conclusion: Hemin affected the expression of Tfh cells in both group thalassemia, but there was no difference of Tfh cell expression between the groups.Keywords: thalassemia disease, T follicular helper, TIGIT, PD-1, interleukin-21https://www.dovepress.com/role-of-hemin-in-the-immune-response-of-t-follicular-helper-lymphocyte-peer-reviewed-fulltext-article-JBMthalassemia diseaset follicular helpertigitpd-1interleukin-21 |
| spellingShingle | Tambunan BA Ugrasena IDG Aryati Role of Hemin in the Immune Response of T Follicular Helper Lymphocytes Expressing T-Cell Immunoreceptor with Immunoglobulin and Immunoreceptor Tyrosine-Based Inhibitory Domains, Programmed Cell Death-1, and Interleukin-21 in Allo-Auto Positive and Negative Thalassemia Journal of Blood Medicine thalassemia disease t follicular helper tigit pd-1 interleukin-21 |
| title | Role of Hemin in the Immune Response of T Follicular Helper Lymphocytes Expressing T-Cell Immunoreceptor with Immunoglobulin and Immunoreceptor Tyrosine-Based Inhibitory Domains, Programmed Cell Death-1, and Interleukin-21 in Allo-Auto Positive and Negative Thalassemia |
| title_full | Role of Hemin in the Immune Response of T Follicular Helper Lymphocytes Expressing T-Cell Immunoreceptor with Immunoglobulin and Immunoreceptor Tyrosine-Based Inhibitory Domains, Programmed Cell Death-1, and Interleukin-21 in Allo-Auto Positive and Negative Thalassemia |
| title_fullStr | Role of Hemin in the Immune Response of T Follicular Helper Lymphocytes Expressing T-Cell Immunoreceptor with Immunoglobulin and Immunoreceptor Tyrosine-Based Inhibitory Domains, Programmed Cell Death-1, and Interleukin-21 in Allo-Auto Positive and Negative Thalassemia |
| title_full_unstemmed | Role of Hemin in the Immune Response of T Follicular Helper Lymphocytes Expressing T-Cell Immunoreceptor with Immunoglobulin and Immunoreceptor Tyrosine-Based Inhibitory Domains, Programmed Cell Death-1, and Interleukin-21 in Allo-Auto Positive and Negative Thalassemia |
| title_short | Role of Hemin in the Immune Response of T Follicular Helper Lymphocytes Expressing T-Cell Immunoreceptor with Immunoglobulin and Immunoreceptor Tyrosine-Based Inhibitory Domains, Programmed Cell Death-1, and Interleukin-21 in Allo-Auto Positive and Negative Thalassemia |
| title_sort | role of hemin in the immune response of t follicular helper lymphocytes expressing t cell immunoreceptor with immunoglobulin and immunoreceptor tyrosine based inhibitory domains programmed cell death 1 and interleukin 21 in allo auto positive and negative thalassemia |
| topic | thalassemia disease t follicular helper tigit pd-1 interleukin-21 |
| url | https://www.dovepress.com/role-of-hemin-in-the-immune-response-of-t-follicular-helper-lymphocyte-peer-reviewed-fulltext-article-JBM |
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