Epigenetic Integrity of Orthodox Seeds Stored under Conventional and Cryogenic Conditions
The level of 5-methylcytosine (m<sup>5</sup>C) in DNA has been observed to change in plants in response to biotic and abiotic stress factors. Little information has been reported on alterations in DNA methylation in orthodox tree seeds in response to storage conditions. In the current st...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
|
| Series: | Forests |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4907/12/3/288 |
| _version_ | 1797415720497184768 |
|---|---|
| author | Beata P. Plitta-Michalak Mirosława Z. Naskręt-Barciszewska Jan Barciszewski Paweł Chmielarz Marcin Michalak |
| author_facet | Beata P. Plitta-Michalak Mirosława Z. Naskręt-Barciszewska Jan Barciszewski Paweł Chmielarz Marcin Michalak |
| author_sort | Beata P. Plitta-Michalak |
| collection | DOAJ |
| description | The level of 5-methylcytosine (m<sup>5</sup>C) in DNA has been observed to change in plants in response to biotic and abiotic stress factors. Little information has been reported on alterations in DNA methylation in orthodox tree seeds in response to storage conditions. In the current study, epigenetic integrity was analyzed in seeds of <i>Pyrus communis</i> L. in response to conventional and cryogenic storage. The results indicate that conventional storage under optimal conditions resulted in a significant increase in m<sup>5</sup>C. In contrast, a decrease in m<sup>5</sup>C level after cryostorage at high water content (WC) was observed, not only in seeds but also in 3-month-old seedlings which were smaller than seedlings obtained from seeds cryostored at optimal WC. This shows that non-optimal cryostorage conditions increase epigenetic instability in seeds and seedlings. Optimal procedures for germplasm conservation are very important for germplasm banking since they have serious implications for the quality of stored collections. Maintaining epigenetic integrity during WC adjustment and optimal storage is a characteristic feature of orthodox seeds. The current results underline the importance of proper protocols and techniques for conventional storage and particularly cryopreservation as a method for conservation of true-to-type germplasm for long periods. |
| first_indexed | 2024-03-09T05:52:35Z |
| format | Article |
| id | doaj.art-c1cb665dc3aa40fa979425739ad75669 |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-09T05:52:35Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forests |
| spelling | doaj.art-c1cb665dc3aa40fa979425739ad756692023-12-03T12:16:04ZengMDPI AGForests1999-49072021-03-0112328810.3390/f12030288Epigenetic Integrity of Orthodox Seeds Stored under Conventional and Cryogenic ConditionsBeata P. Plitta-Michalak0Mirosława Z. Naskręt-Barciszewska1Jan Barciszewski2Paweł Chmielarz3Marcin Michalak4Department of Plant Physiology, Genetics and Biotechnology, University of Warmia and Mazury in Olsztyn, M Oczapowskiego 1A, 10-721 Olsztyn, PolandInstitute of Bioorganic Chemistry, Polish Academy of Sciences, Z. Noskowskiego 12/14, 61-704 Poznań, PolandInstitute of Bioorganic Chemistry, Polish Academy of Sciences, Z. Noskowskiego 12/14, 61-704 Poznań, PolandInstitute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, PolandDepartment of Plant Physiology, Genetics and Biotechnology, University of Warmia and Mazury in Olsztyn, M Oczapowskiego 1A, 10-721 Olsztyn, PolandThe level of 5-methylcytosine (m<sup>5</sup>C) in DNA has been observed to change in plants in response to biotic and abiotic stress factors. Little information has been reported on alterations in DNA methylation in orthodox tree seeds in response to storage conditions. In the current study, epigenetic integrity was analyzed in seeds of <i>Pyrus communis</i> L. in response to conventional and cryogenic storage. The results indicate that conventional storage under optimal conditions resulted in a significant increase in m<sup>5</sup>C. In contrast, a decrease in m<sup>5</sup>C level after cryostorage at high water content (WC) was observed, not only in seeds but also in 3-month-old seedlings which were smaller than seedlings obtained from seeds cryostored at optimal WC. This shows that non-optimal cryostorage conditions increase epigenetic instability in seeds and seedlings. Optimal procedures for germplasm conservation are very important for germplasm banking since they have serious implications for the quality of stored collections. Maintaining epigenetic integrity during WC adjustment and optimal storage is a characteristic feature of orthodox seeds. The current results underline the importance of proper protocols and techniques for conventional storage and particularly cryopreservation as a method for conservation of true-to-type germplasm for long periods.https://www.mdpi.com/1999-4907/12/3/288conventional storagecryopreservationdesiccationDNA methylationepigenetic integrityliquid nitrogen |
| spellingShingle | Beata P. Plitta-Michalak Mirosława Z. Naskręt-Barciszewska Jan Barciszewski Paweł Chmielarz Marcin Michalak Epigenetic Integrity of Orthodox Seeds Stored under Conventional and Cryogenic Conditions Forests conventional storage cryopreservation desiccation DNA methylation epigenetic integrity liquid nitrogen |
| title | Epigenetic Integrity of Orthodox Seeds Stored under Conventional and Cryogenic Conditions |
| title_full | Epigenetic Integrity of Orthodox Seeds Stored under Conventional and Cryogenic Conditions |
| title_fullStr | Epigenetic Integrity of Orthodox Seeds Stored under Conventional and Cryogenic Conditions |
| title_full_unstemmed | Epigenetic Integrity of Orthodox Seeds Stored under Conventional and Cryogenic Conditions |
| title_short | Epigenetic Integrity of Orthodox Seeds Stored under Conventional and Cryogenic Conditions |
| title_sort | epigenetic integrity of orthodox seeds stored under conventional and cryogenic conditions |
| topic | conventional storage cryopreservation desiccation DNA methylation epigenetic integrity liquid nitrogen |
| url | https://www.mdpi.com/1999-4907/12/3/288 |
| work_keys_str_mv | AT beatapplittamichalak epigeneticintegrityoforthodoxseedsstoredunderconventionalandcryogenicconditions AT mirosławaznaskretbarciszewska epigeneticintegrityoforthodoxseedsstoredunderconventionalandcryogenicconditions AT janbarciszewski epigeneticintegrityoforthodoxseedsstoredunderconventionalandcryogenicconditions AT pawełchmielarz epigeneticintegrityoforthodoxseedsstoredunderconventionalandcryogenicconditions AT marcinmichalak epigeneticintegrityoforthodoxseedsstoredunderconventionalandcryogenicconditions |