Effects of Sasanquasaponin on Ischemia and Reperfusion Injury in Mouse Hearts
We investigated effects of sasanquasaponin (SQS), a traditional Chinese herb’s effective component, on ischemia and reperfusion injury in mouse hearts and the possible role of intracellular Cl- homeostasis on SQS’s protective effects during ischemia and reperfusion. An in vivo experimental ischemia...
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| Format: | Article |
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Elsevier
2004-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319324995 |
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| author | Zhong-Fang Lai Zhanqiang Shao Yu-Zhen Chen Ming He Qiren Huang Katsuhide Nishi |
| author_facet | Zhong-Fang Lai Zhanqiang Shao Yu-Zhen Chen Ming He Qiren Huang Katsuhide Nishi |
| author_sort | Zhong-Fang Lai |
| collection | DOAJ |
| description | We investigated effects of sasanquasaponin (SQS), a traditional Chinese herb’s effective component, on ischemia and reperfusion injury in mouse hearts and the possible role of intracellular Cl- homeostasis on SQS’s protective effects during ischemia and reperfusion. An in vivo experimental ischemia model was made in mice (weight 27 – 45 g) using ligation of left anterior descending coronary artery, and in vitro models were made in perfused hearts by stopping flow or in isolated ventricular myocytes by hypoxia. The in vivo results showed that SQS inhibited cardiac arrhythmias during ischemia and reperfusion. Incidence of arrhythmias during ischemia and reperfusion, including ventricular premature beats and ventricular fibrillation, was significantly decreased in the SQS-pretreated group (P<0.05). Results in perfused hearts showed that SQS suppressed the arrhythmias, prevented against ischemia-induced decrease in contract force and promoted the force recovery from reperfusion. Furthermore, intracellular Cl- concentrations ([Cl-]i) were measured using a MQAE fluorescence method in isolated ventricular myocytes in vitro. SQS slightly decreased [Cl-]i in non-hypoxic myocytes and delayed the hypoxia/reoxygenation-induced increase in [Cl-]i during ischemia and reperfusion (P<0.05). Our results showed that SQS protected against ischemia/reperfusion-induced cardiac injury in mouse hearts and that modulation of intracellular Cl- homeostasis by SQS would play a role in its anti-arrhythmia effects during ischemia and reperfusion. Keywords:: sasanquasaponin, ischemia and reperfusion injury, arrhythmia, intracellular Cl- concentration, left anterior descending coronary artery |
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| id | doaj.art-c1d15f98ed7943e4aa67b8e54b216a7c |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-23T20:40:30Z |
| publishDate | 2004-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-c1d15f98ed7943e4aa67b8e54b216a7c2022-12-21T17:31:56ZengElsevierJournal of Pharmacological Sciences1347-86132004-01-01943313324Effects of Sasanquasaponin on Ischemia and Reperfusion Injury in Mouse HeartsZhong-Fang Lai0Zhanqiang Shao1Yu-Zhen Chen2Ming He3Qiren Huang4Katsuhide Nishi5Departments of Cell and Biological Pharmacology, Kumamoto University, Kumamoto 860-8556, Japan; Corresponding author. FAX: +81-96-373-5082 E-mail: lai-zf@gpo.kumamoto-u.ac.jpDepartments of Cell and Biological Pharmacology, Kumamoto University, Kumamoto 860-8556, Japan; Departments of Cardiovascular Surgery, Kumamoto University, Kumamoto 860-8556, JapanDepartments of Immunogenetics, Graduate School of Medical Sciences, Kumamoto University, Kumamoto 860-8556, JapanDepartment of Pharmacology, Jiangxi Medical College, Nanchang 330006, ChinaDepartment of Pharmacology, Jiangxi Medical College, Nanchang 330006, ChinaDepartments of Cell and Biological Pharmacology, Kumamoto University, Kumamoto 860-8556, JapanWe investigated effects of sasanquasaponin (SQS), a traditional Chinese herb’s effective component, on ischemia and reperfusion injury in mouse hearts and the possible role of intracellular Cl- homeostasis on SQS’s protective effects during ischemia and reperfusion. An in vivo experimental ischemia model was made in mice (weight 27 – 45 g) using ligation of left anterior descending coronary artery, and in vitro models were made in perfused hearts by stopping flow or in isolated ventricular myocytes by hypoxia. The in vivo results showed that SQS inhibited cardiac arrhythmias during ischemia and reperfusion. Incidence of arrhythmias during ischemia and reperfusion, including ventricular premature beats and ventricular fibrillation, was significantly decreased in the SQS-pretreated group (P<0.05). Results in perfused hearts showed that SQS suppressed the arrhythmias, prevented against ischemia-induced decrease in contract force and promoted the force recovery from reperfusion. Furthermore, intracellular Cl- concentrations ([Cl-]i) were measured using a MQAE fluorescence method in isolated ventricular myocytes in vitro. SQS slightly decreased [Cl-]i in non-hypoxic myocytes and delayed the hypoxia/reoxygenation-induced increase in [Cl-]i during ischemia and reperfusion (P<0.05). Our results showed that SQS protected against ischemia/reperfusion-induced cardiac injury in mouse hearts and that modulation of intracellular Cl- homeostasis by SQS would play a role in its anti-arrhythmia effects during ischemia and reperfusion. Keywords:: sasanquasaponin, ischemia and reperfusion injury, arrhythmia, intracellular Cl- concentration, left anterior descending coronary arteryhttp://www.sciencedirect.com/science/article/pii/S1347861319324995 |
| spellingShingle | Zhong-Fang Lai Zhanqiang Shao Yu-Zhen Chen Ming He Qiren Huang Katsuhide Nishi Effects of Sasanquasaponin on Ischemia and Reperfusion Injury in Mouse Hearts Journal of Pharmacological Sciences |
| title | Effects of Sasanquasaponin on Ischemia and Reperfusion Injury in Mouse Hearts |
| title_full | Effects of Sasanquasaponin on Ischemia and Reperfusion Injury in Mouse Hearts |
| title_fullStr | Effects of Sasanquasaponin on Ischemia and Reperfusion Injury in Mouse Hearts |
| title_full_unstemmed | Effects of Sasanquasaponin on Ischemia and Reperfusion Injury in Mouse Hearts |
| title_short | Effects of Sasanquasaponin on Ischemia and Reperfusion Injury in Mouse Hearts |
| title_sort | effects of sasanquasaponin on ischemia and reperfusion injury in mouse hearts |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319324995 |
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