Improved Ethanol Production Based on High Solids Fed-Batch Simultaneous Saccharification and Fermentation with Alkali-Pretreated Sugarcane Bagasse
Alkali-pretreated sugarcane bagasse fiber was subjected to fed-batch simultaneous saccharification and fermentation (SSF) with a pre-hydrolysis process to increase the solids loading and produce a high concentration of ethanol. The hydrolysis medium and yeast feeding modes were investigated to deter...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
North Carolina State University
2016-01-01
|
| Series: | BioResources |
| Subjects: | |
| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_1_2548_Liu_Improved_Ethanol_Production_High_Solids |
| _version_ | 1819097555024216064 |
|---|---|
| author | Yunyun Liu Jingliang Xu Xu Yu Zhang Minchao He Cuiyi Liang Zhenhong Yuan Jun Xie |
| author_facet | Yunyun Liu Jingliang Xu Xu Yu Zhang Minchao He Cuiyi Liang Zhenhong Yuan Jun Xie |
| author_sort | Yunyun Liu |
| collection | DOAJ |
| description | Alkali-pretreated sugarcane bagasse fiber was subjected to fed-batch simultaneous saccharification and fermentation (SSF) with a pre-hydrolysis process to increase the solids loading and produce a high concentration of ethanol. The hydrolysis medium and yeast feeding modes were investigated to determine suitable conditions for high sugar yield and ethanol production. Batch addition resulted in a cumulative substrate concentration of up to 36% (w/v) and enhanced ethanol concentrations, while ethanol conversion efficiency gradually declined. Enzymatic pre-hydrolysis and fermentation with fed-batch mode contributed to the SSF process. The highest ethanol concentration was 66.915 g/L with the conversion efficiency of 72.89%, which was achieved at 30% (w/v) solids content after 96 h of fermentation. Hydrolyzed medium and yeast were added in batch mode at 24 h of enzymatic hydrolysis and fermentation, respectively. Thus, combining the fed-batch mode with pre-hydrolysis SSF produced a high yield of ethanol. |
| first_indexed | 2024-12-22T00:16:57Z |
| format | Article |
| id | doaj.art-c1d5d361df614ca9a4d8364b64ae7165 |
| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-12-22T00:16:57Z |
| publishDate | 2016-01-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-c1d5d361df614ca9a4d8364b64ae71652022-12-21T18:45:16ZengNorth Carolina State UniversityBioResources1930-21261930-21262016-01-011112548255610.15376/biores.11.1.2548-2556Improved Ethanol Production Based on High Solids Fed-Batch Simultaneous Saccharification and Fermentation with Alkali-Pretreated Sugarcane BagasseYunyun Liu0Jingliang Xu Xu1Yu Zhang2Minchao He3Cuiyi Liang4Zhenhong Yuan5Jun Xie6Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; ChinaKey Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; ChinaKey Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; ChinaKey Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; ChinaKey Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; ChinaKey Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; ChinaInstitute of New Energy and New Materials, South China Agriculture University, Guangzhou, 510642, P. R. China; ChinaAlkali-pretreated sugarcane bagasse fiber was subjected to fed-batch simultaneous saccharification and fermentation (SSF) with a pre-hydrolysis process to increase the solids loading and produce a high concentration of ethanol. The hydrolysis medium and yeast feeding modes were investigated to determine suitable conditions for high sugar yield and ethanol production. Batch addition resulted in a cumulative substrate concentration of up to 36% (w/v) and enhanced ethanol concentrations, while ethanol conversion efficiency gradually declined. Enzymatic pre-hydrolysis and fermentation with fed-batch mode contributed to the SSF process. The highest ethanol concentration was 66.915 g/L with the conversion efficiency of 72.89%, which was achieved at 30% (w/v) solids content after 96 h of fermentation. Hydrolyzed medium and yeast were added in batch mode at 24 h of enzymatic hydrolysis and fermentation, respectively. Thus, combining the fed-batch mode with pre-hydrolysis SSF produced a high yield of ethanol.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_1_2548_Liu_Improved_Ethanol_Production_High_SolidsSimultaneous saccharification and fermentationPre-hydrolysisFed-batch processBioethanolSugarcane bagasse |
| spellingShingle | Yunyun Liu Jingliang Xu Xu Yu Zhang Minchao He Cuiyi Liang Zhenhong Yuan Jun Xie Improved Ethanol Production Based on High Solids Fed-Batch Simultaneous Saccharification and Fermentation with Alkali-Pretreated Sugarcane Bagasse BioResources Simultaneous saccharification and fermentation Pre-hydrolysis Fed-batch process Bioethanol Sugarcane bagasse |
| title | Improved Ethanol Production Based on High Solids Fed-Batch Simultaneous Saccharification and Fermentation with Alkali-Pretreated Sugarcane Bagasse |
| title_full | Improved Ethanol Production Based on High Solids Fed-Batch Simultaneous Saccharification and Fermentation with Alkali-Pretreated Sugarcane Bagasse |
| title_fullStr | Improved Ethanol Production Based on High Solids Fed-Batch Simultaneous Saccharification and Fermentation with Alkali-Pretreated Sugarcane Bagasse |
| title_full_unstemmed | Improved Ethanol Production Based on High Solids Fed-Batch Simultaneous Saccharification and Fermentation with Alkali-Pretreated Sugarcane Bagasse |
| title_short | Improved Ethanol Production Based on High Solids Fed-Batch Simultaneous Saccharification and Fermentation with Alkali-Pretreated Sugarcane Bagasse |
| title_sort | improved ethanol production based on high solids fed batch simultaneous saccharification and fermentation with alkali pretreated sugarcane bagasse |
| topic | Simultaneous saccharification and fermentation Pre-hydrolysis Fed-batch process Bioethanol Sugarcane bagasse |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_1_2548_Liu_Improved_Ethanol_Production_High_Solids |
| work_keys_str_mv | AT yunyunliu improvedethanolproductionbasedonhighsolidsfedbatchsimultaneoussaccharificationandfermentationwithalkalipretreatedsugarcanebagasse AT jingliangxuxu improvedethanolproductionbasedonhighsolidsfedbatchsimultaneoussaccharificationandfermentationwithalkalipretreatedsugarcanebagasse AT yuzhang improvedethanolproductionbasedonhighsolidsfedbatchsimultaneoussaccharificationandfermentationwithalkalipretreatedsugarcanebagasse AT minchaohe improvedethanolproductionbasedonhighsolidsfedbatchsimultaneoussaccharificationandfermentationwithalkalipretreatedsugarcanebagasse AT cuiyiliang improvedethanolproductionbasedonhighsolidsfedbatchsimultaneoussaccharificationandfermentationwithalkalipretreatedsugarcanebagasse AT zhenhongyuan improvedethanolproductionbasedonhighsolidsfedbatchsimultaneoussaccharificationandfermentationwithalkalipretreatedsugarcanebagasse AT junxie improvedethanolproductionbasedonhighsolidsfedbatchsimultaneoussaccharificationandfermentationwithalkalipretreatedsugarcanebagasse |