Antioxidant effect of nicotinamide mononucleotide in tendinopathy
Abstract Background A link between tendinopathy and oxidative stress has been recently reported. Nicotinamide mononucleotide (NMN) is a precursor of nicotinamide adenine dinucleotide, which plays an important role in cell redox homeostasis. The aim of this study was to evaluate the antioxidant effec...
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| Format: | Article |
| Language: | English |
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BMC
2022-03-01
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| Series: | BMC Musculoskeletal Disorders |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12891-022-05205-z |
| _version_ | 1828871776705183744 |
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| author | Kohei Yamaura Yutaka Mifune Atsuyuki Inui Hanako Nishimoto Takashi Kurosawa Shintaro Mukohara Yuichi Hoshino Takahiro Niikura Ryosuke Kuroda |
| author_facet | Kohei Yamaura Yutaka Mifune Atsuyuki Inui Hanako Nishimoto Takashi Kurosawa Shintaro Mukohara Yuichi Hoshino Takahiro Niikura Ryosuke Kuroda |
| author_sort | Kohei Yamaura |
| collection | DOAJ |
| description | Abstract Background A link between tendinopathy and oxidative stress has been recently reported. Nicotinamide mononucleotide (NMN) is a precursor of nicotinamide adenine dinucleotide, which plays an important role in cell redox homeostasis. The aim of this study was to evaluate the antioxidant effect of NMN on tendinopathy in vitro and in vivo. Methods Tenocytes from healthy Sprague-Dawley rats were cultured in regular glucose (RG) and high-glucose (HG) conditions with or without NMN, and were divided into four groups: RG NMN(−), RG NMN(+), HG NMN(−), and HG NMN(+). Cell viability, reactive oxygen species (ROS) accumulation, apoptotic rate, and mRNA expression of nicotinamide adenine dinucleotide phosphate oxidase (NOX)1, NOX4, interleukin (IL)6, sirtuin (SIRT)1, and SIRT6 were investigated. In addition, rats with collagenase-induced tendinopathy were treated with or without NMN. Immunostaining of NOX1 and NOX4; mRNA expression of SIRT1, SIRT6, and IL6; and superoxide dismutase (SOD) activity measurements in the Achilles tendon were performed. Results NMN increased the expression of SIRT1 and SIRT6 in rat tenocytes, but decreased the levels of NOX1, NOX4, IL6, ROS, and apoptosis. In Achilles tendons with collagenase-induced tendinopathy, NMN increased the mRNA expression of SIRT1 and SIRT6, as well as SOD activity; while suppressing protein expression of NOX1 and NOX4, and mRNA expression of IL6. Conclusion The in vitro and in vivo results of this study show that NMN exerts an antioxidant effect on tendinopathy by promoting the expression of SIRT while inhibiting that of NOX. |
| first_indexed | 2024-12-13T06:40:19Z |
| format | Article |
| id | doaj.art-c1da47ee23084f898e14284254a66f38 |
| institution | Directory Open Access Journal |
| issn | 1471-2474 |
| language | English |
| last_indexed | 2024-12-13T06:40:19Z |
| publishDate | 2022-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj.art-c1da47ee23084f898e14284254a66f382022-12-21T23:56:26ZengBMCBMC Musculoskeletal Disorders1471-24742022-03-0123111010.1186/s12891-022-05205-zAntioxidant effect of nicotinamide mononucleotide in tendinopathyKohei Yamaura0Yutaka Mifune1Atsuyuki Inui2Hanako Nishimoto3Takashi Kurosawa4Shintaro Mukohara5Yuichi Hoshino6Takahiro Niikura7Ryosuke Kuroda8Department of Orthopaedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopaedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopaedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopaedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopaedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopaedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopaedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopaedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopaedic Surgery, Kobe University Graduate School of MedicineAbstract Background A link between tendinopathy and oxidative stress has been recently reported. Nicotinamide mononucleotide (NMN) is a precursor of nicotinamide adenine dinucleotide, which plays an important role in cell redox homeostasis. The aim of this study was to evaluate the antioxidant effect of NMN on tendinopathy in vitro and in vivo. Methods Tenocytes from healthy Sprague-Dawley rats were cultured in regular glucose (RG) and high-glucose (HG) conditions with or without NMN, and were divided into four groups: RG NMN(−), RG NMN(+), HG NMN(−), and HG NMN(+). Cell viability, reactive oxygen species (ROS) accumulation, apoptotic rate, and mRNA expression of nicotinamide adenine dinucleotide phosphate oxidase (NOX)1, NOX4, interleukin (IL)6, sirtuin (SIRT)1, and SIRT6 were investigated. In addition, rats with collagenase-induced tendinopathy were treated with or without NMN. Immunostaining of NOX1 and NOX4; mRNA expression of SIRT1, SIRT6, and IL6; and superoxide dismutase (SOD) activity measurements in the Achilles tendon were performed. Results NMN increased the expression of SIRT1 and SIRT6 in rat tenocytes, but decreased the levels of NOX1, NOX4, IL6, ROS, and apoptosis. In Achilles tendons with collagenase-induced tendinopathy, NMN increased the mRNA expression of SIRT1 and SIRT6, as well as SOD activity; while suppressing protein expression of NOX1 and NOX4, and mRNA expression of IL6. Conclusion The in vitro and in vivo results of this study show that NMN exerts an antioxidant effect on tendinopathy by promoting the expression of SIRT while inhibiting that of NOX.https://doi.org/10.1186/s12891-022-05205-zNicotinamide mononucleotideOxidative stressSIRTTendinopathy |
| spellingShingle | Kohei Yamaura Yutaka Mifune Atsuyuki Inui Hanako Nishimoto Takashi Kurosawa Shintaro Mukohara Yuichi Hoshino Takahiro Niikura Ryosuke Kuroda Antioxidant effect of nicotinamide mononucleotide in tendinopathy BMC Musculoskeletal Disorders Nicotinamide mononucleotide Oxidative stress SIRT Tendinopathy |
| title | Antioxidant effect of nicotinamide mononucleotide in tendinopathy |
| title_full | Antioxidant effect of nicotinamide mononucleotide in tendinopathy |
| title_fullStr | Antioxidant effect of nicotinamide mononucleotide in tendinopathy |
| title_full_unstemmed | Antioxidant effect of nicotinamide mononucleotide in tendinopathy |
| title_short | Antioxidant effect of nicotinamide mononucleotide in tendinopathy |
| title_sort | antioxidant effect of nicotinamide mononucleotide in tendinopathy |
| topic | Nicotinamide mononucleotide Oxidative stress SIRT Tendinopathy |
| url | https://doi.org/10.1186/s12891-022-05205-z |
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