A Genomic and Transcriptomic Overview of MATE, ABC, and MFS Transporters in <i>Citrus sinensis</i> Interaction with <i>Xanthomonas citri</i> subsp. <i>citri</i>
The multi-antimicrobial extrusion (MATE), ATP-binding cassette (ABC), and major facilitator superfamily (MFS) are the main plant transporters families, playing an essential role in the membrane-trafficking network and plant-defense mechanism. The citrus canker type A (CC), is a devastating disease c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/6/794 |
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| author | Maria H. M. Julião Saura R. Silva Jesus A. Ferro Alessandro M. Varani |
| author_facet | Maria H. M. Julião Saura R. Silva Jesus A. Ferro Alessandro M. Varani |
| author_sort | Maria H. M. Julião |
| collection | DOAJ |
| description | The multi-antimicrobial extrusion (MATE), ATP-binding cassette (ABC), and major facilitator superfamily (MFS) are the main plant transporters families, playing an essential role in the membrane-trafficking network and plant-defense mechanism. The citrus canker type A (CC), is a devastating disease caused by <i>Xanthomonas citri</i> subsp. <i>citri</i> (Xac), affecting all citrus species. In this work, we performed an in silico analysis of genes and transcripts from MATE, ABC, and MFS families to infer the role of membrane transporters in Citrus-Xac interaction. Using as reference, the available <i>Citrus sinensis</i> genome and the citrus reference transcriptome from CitrusKB database, 67 MATE, 91 MFS, and 143 ABC genes and 82 MATE, 139 MFS, and 226 ABC transcripts were identified and classified into subfamilies. Duplications, alternative-splicing, and potentially non-transcribed transporters’ genes were revealed. Interestingly, MATE I and ABC G subfamilies appear differently regulated during Xac infection. Furthermore, <i>Citrus</i> spp. showing distinct levels of CC susceptibility exhibited different sets of transporters transcripts, supporting dissimilar molecular patterns of membrane transporters in Citrus-Xac interaction. According to our findings, 4 MATE, 10 ABC, and 3 MFS are potentially related to plant-defense mechanisms. Overall, this work provides an extensive analysis of MATE, ABC, and MFS transporters’ in Citrus-Xac interaction, bringing new insights on membrane transporters in plant-pathogen interactions. |
| first_indexed | 2024-03-10T18:54:37Z |
| format | Article |
| id | doaj.art-c1db9083e79a426ea021ad358e4829ad |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T18:54:37Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-c1db9083e79a426ea021ad358e4829ad2023-11-20T04:55:13ZengMDPI AGPlants2223-77472020-06-019679410.3390/plants9060794A Genomic and Transcriptomic Overview of MATE, ABC, and MFS Transporters in <i>Citrus sinensis</i> Interaction with <i>Xanthomonas citri</i> subsp. <i>citri</i>Maria H. M. Julião0Saura R. Silva1Jesus A. Ferro2Alessandro M. Varani3Department of Technology, School of Agricultural and Veterinary Sciences, São Paulo State University, Jaboticabal 14884-900, BrazilDepartment of Technology, School of Agricultural and Veterinary Sciences, São Paulo State University, Jaboticabal 14884-900, BrazilDepartment of Technology, School of Agricultural and Veterinary Sciences, São Paulo State University, Jaboticabal 14884-900, BrazilDepartment of Technology, School of Agricultural and Veterinary Sciences, São Paulo State University, Jaboticabal 14884-900, BrazilThe multi-antimicrobial extrusion (MATE), ATP-binding cassette (ABC), and major facilitator superfamily (MFS) are the main plant transporters families, playing an essential role in the membrane-trafficking network and plant-defense mechanism. The citrus canker type A (CC), is a devastating disease caused by <i>Xanthomonas citri</i> subsp. <i>citri</i> (Xac), affecting all citrus species. In this work, we performed an in silico analysis of genes and transcripts from MATE, ABC, and MFS families to infer the role of membrane transporters in Citrus-Xac interaction. Using as reference, the available <i>Citrus sinensis</i> genome and the citrus reference transcriptome from CitrusKB database, 67 MATE, 91 MFS, and 143 ABC genes and 82 MATE, 139 MFS, and 226 ABC transcripts were identified and classified into subfamilies. Duplications, alternative-splicing, and potentially non-transcribed transporters’ genes were revealed. Interestingly, MATE I and ABC G subfamilies appear differently regulated during Xac infection. Furthermore, <i>Citrus</i> spp. showing distinct levels of CC susceptibility exhibited different sets of transporters transcripts, supporting dissimilar molecular patterns of membrane transporters in Citrus-Xac interaction. According to our findings, 4 MATE, 10 ABC, and 3 MFS are potentially related to plant-defense mechanisms. Overall, this work provides an extensive analysis of MATE, ABC, and MFS transporters’ in Citrus-Xac interaction, bringing new insights on membrane transporters in plant-pathogen interactions.https://www.mdpi.com/2223-7747/9/6/794citrus cankercomparative genomicsmembrane transportersdifferentially expressed genesplant-pathogen interactions |
| spellingShingle | Maria H. M. Julião Saura R. Silva Jesus A. Ferro Alessandro M. Varani A Genomic and Transcriptomic Overview of MATE, ABC, and MFS Transporters in <i>Citrus sinensis</i> Interaction with <i>Xanthomonas citri</i> subsp. <i>citri</i> Plants citrus canker comparative genomics membrane transporters differentially expressed genes plant-pathogen interactions |
| title | A Genomic and Transcriptomic Overview of MATE, ABC, and MFS Transporters in <i>Citrus sinensis</i> Interaction with <i>Xanthomonas citri</i> subsp. <i>citri</i> |
| title_full | A Genomic and Transcriptomic Overview of MATE, ABC, and MFS Transporters in <i>Citrus sinensis</i> Interaction with <i>Xanthomonas citri</i> subsp. <i>citri</i> |
| title_fullStr | A Genomic and Transcriptomic Overview of MATE, ABC, and MFS Transporters in <i>Citrus sinensis</i> Interaction with <i>Xanthomonas citri</i> subsp. <i>citri</i> |
| title_full_unstemmed | A Genomic and Transcriptomic Overview of MATE, ABC, and MFS Transporters in <i>Citrus sinensis</i> Interaction with <i>Xanthomonas citri</i> subsp. <i>citri</i> |
| title_short | A Genomic and Transcriptomic Overview of MATE, ABC, and MFS Transporters in <i>Citrus sinensis</i> Interaction with <i>Xanthomonas citri</i> subsp. <i>citri</i> |
| title_sort | genomic and transcriptomic overview of mate abc and mfs transporters in i citrus sinensis i interaction with i xanthomonas citri i subsp i citri i |
| topic | citrus canker comparative genomics membrane transporters differentially expressed genes plant-pathogen interactions |
| url | https://www.mdpi.com/2223-7747/9/6/794 |
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