Transcriptome Profiling of the Salt Stress Response in the Leaves and Roots of Halophytic Eutrema salsugineum
Eutrema salsugineum can grow in natural harsh environments; however, the underlying mechanisms for salt tolerance of Eutrema need to be further understood. Herein, the transcriptome profiling of Eutrema leaves and roots exposed to 300 mM NaCl is investigated, and the result emphasized the role of ge...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-11-01
|
| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.770742/full |
| _version_ | 1828146603991498752 |
|---|---|
| author | Chuanshun Li Yuting Qi Chuanzhi Zhao Chuanzhi Zhao Xingjun Wang Xingjun Wang Quan Zhang |
| author_facet | Chuanshun Li Yuting Qi Chuanzhi Zhao Chuanzhi Zhao Xingjun Wang Xingjun Wang Quan Zhang |
| author_sort | Chuanshun Li |
| collection | DOAJ |
| description | Eutrema salsugineum can grow in natural harsh environments; however, the underlying mechanisms for salt tolerance of Eutrema need to be further understood. Herein, the transcriptome profiling of Eutrema leaves and roots exposed to 300 mM NaCl is investigated, and the result emphasized the role of genes involved in lignin biosynthesis, autophagy, peroxisome, and sugar metabolism upon salt stress. Furthermore, the expression of the lignin biosynthesis and autophagy-related genes, as well as 16 random selected genes, was validated by qRT-PCR. Notably, the transcript abundance of a large number of lignin biosynthesis genes such as CCoAOMT, C4H, CCR, CAD, POD, and C3′H in leaves was markedly elevated by salt shock. And the examined lignin content in leaves and roots demonstrated salt stress led to lignin accumulation, which indicated the enhanced lignin level could be an important mechanism for Eutrema responding to salt stress. Additionally, the differentially expressed genes (DEGs) assigned in the autophagy pathway including Vac8, Atg8, and Atg4, as well as DEGs enriched in the peroxisome pathway such as EsPEX7, EsCAT, and EsSOD2, were markedly induced in leaves and/or roots. In sugar metabolism pathways, the transcript levels of most DEGs associated with the synthesis of sucrose, trehalose, raffinose, and xylose were significantly enhanced. Furthermore, the expression of various stress-related transcription factor genes including WRKY, AP2/ERF-ERF, NAC, bZIP, MYB, C2H2, and HSF was strikingly improved. Collectively, the increased expression of biosynthesis genes of lignin and soluble sugars, as well as the genes in the autophagy and peroxisome pathways, suggested that Eutrema encountering salt shock possibly possess a higher capacity to adjust osmotically and facilitate water transport and scavenge reactive oxidative species and oxidative proteins to cope with the salt environment. Thus, this study provides a new insight for exploring the salt tolerance mechanism of halophytic Eutrema and discovering new gene targets for the genetic improvement of crops. |
| first_indexed | 2024-04-11T20:49:25Z |
| format | Article |
| id | doaj.art-c1dd353947e64828bff312a742726f79 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-11T20:49:25Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-c1dd353947e64828bff312a742726f792022-12-22T04:03:53ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-11-011210.3389/fgene.2021.770742770742Transcriptome Profiling of the Salt Stress Response in the Leaves and Roots of Halophytic Eutrema salsugineumChuanshun Li0Yuting Qi1Chuanzhi Zhao2Chuanzhi Zhao3Xingjun Wang4Xingjun Wang5Quan Zhang6Shandong Provincial Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, Jinan, ChinaShandong Provincial Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, Jinan, ChinaShandong Provincial Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, Jinan, ChinaBio-Tech Research Center, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, ChinaShandong Provincial Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, Jinan, ChinaBio-Tech Research Center, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, ChinaShandong Provincial Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, Jinan, ChinaEutrema salsugineum can grow in natural harsh environments; however, the underlying mechanisms for salt tolerance of Eutrema need to be further understood. Herein, the transcriptome profiling of Eutrema leaves and roots exposed to 300 mM NaCl is investigated, and the result emphasized the role of genes involved in lignin biosynthesis, autophagy, peroxisome, and sugar metabolism upon salt stress. Furthermore, the expression of the lignin biosynthesis and autophagy-related genes, as well as 16 random selected genes, was validated by qRT-PCR. Notably, the transcript abundance of a large number of lignin biosynthesis genes such as CCoAOMT, C4H, CCR, CAD, POD, and C3′H in leaves was markedly elevated by salt shock. And the examined lignin content in leaves and roots demonstrated salt stress led to lignin accumulation, which indicated the enhanced lignin level could be an important mechanism for Eutrema responding to salt stress. Additionally, the differentially expressed genes (DEGs) assigned in the autophagy pathway including Vac8, Atg8, and Atg4, as well as DEGs enriched in the peroxisome pathway such as EsPEX7, EsCAT, and EsSOD2, were markedly induced in leaves and/or roots. In sugar metabolism pathways, the transcript levels of most DEGs associated with the synthesis of sucrose, trehalose, raffinose, and xylose were significantly enhanced. Furthermore, the expression of various stress-related transcription factor genes including WRKY, AP2/ERF-ERF, NAC, bZIP, MYB, C2H2, and HSF was strikingly improved. Collectively, the increased expression of biosynthesis genes of lignin and soluble sugars, as well as the genes in the autophagy and peroxisome pathways, suggested that Eutrema encountering salt shock possibly possess a higher capacity to adjust osmotically and facilitate water transport and scavenge reactive oxidative species and oxidative proteins to cope with the salt environment. Thus, this study provides a new insight for exploring the salt tolerance mechanism of halophytic Eutrema and discovering new gene targets for the genetic improvement of crops.https://www.frontiersin.org/articles/10.3389/fgene.2021.770742/fullEutrema salsugineumRNA-seqsalt shockautophagylignin biosynthesisperoxisome |
| spellingShingle | Chuanshun Li Yuting Qi Chuanzhi Zhao Chuanzhi Zhao Xingjun Wang Xingjun Wang Quan Zhang Transcriptome Profiling of the Salt Stress Response in the Leaves and Roots of Halophytic Eutrema salsugineum Frontiers in Genetics Eutrema salsugineum RNA-seq salt shock autophagy lignin biosynthesis peroxisome |
| title | Transcriptome Profiling of the Salt Stress Response in the Leaves and Roots of Halophytic Eutrema salsugineum |
| title_full | Transcriptome Profiling of the Salt Stress Response in the Leaves and Roots of Halophytic Eutrema salsugineum |
| title_fullStr | Transcriptome Profiling of the Salt Stress Response in the Leaves and Roots of Halophytic Eutrema salsugineum |
| title_full_unstemmed | Transcriptome Profiling of the Salt Stress Response in the Leaves and Roots of Halophytic Eutrema salsugineum |
| title_short | Transcriptome Profiling of the Salt Stress Response in the Leaves and Roots of Halophytic Eutrema salsugineum |
| title_sort | transcriptome profiling of the salt stress response in the leaves and roots of halophytic eutrema salsugineum |
| topic | Eutrema salsugineum RNA-seq salt shock autophagy lignin biosynthesis peroxisome |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2021.770742/full |
| work_keys_str_mv | AT chuanshunli transcriptomeprofilingofthesaltstressresponseintheleavesandrootsofhalophyticeutremasalsugineum AT yutingqi transcriptomeprofilingofthesaltstressresponseintheleavesandrootsofhalophyticeutremasalsugineum AT chuanzhizhao transcriptomeprofilingofthesaltstressresponseintheleavesandrootsofhalophyticeutremasalsugineum AT chuanzhizhao transcriptomeprofilingofthesaltstressresponseintheleavesandrootsofhalophyticeutremasalsugineum AT xingjunwang transcriptomeprofilingofthesaltstressresponseintheleavesandrootsofhalophyticeutremasalsugineum AT xingjunwang transcriptomeprofilingofthesaltstressresponseintheleavesandrootsofhalophyticeutremasalsugineum AT quanzhang transcriptomeprofilingofthesaltstressresponseintheleavesandrootsofhalophyticeutremasalsugineum |