Proteomic Analysis of Liver from Finishing Beef Cattle Supplemented with a Rumen-Protected B-Vitamin Blend and Hydroxy Trace Minerals
Vitamin B and trace minerals are crucial molecular signals involved in many biological pathways; however, their bioavailability is compromised in high-producing ruminant animals. So far, studies have mainly focused on the effects of these micronutrients on animal performance, but their use in a rume...
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MDPI AG
2021-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/7/1934 |
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| author | Mariana Mescouto Lopes Thaís Ribeiro Brito Josiane Fonseca Lage Thaís Correia Costa Marta Maria dos Santos Fontes Nick Vergara Lopes Serão Tiago Antônio de Oliveira Mendes Ricardo Andrade Reis Renata Veroneze Fabyano Fonseca e Silva Marcio de Souza Duarte |
| author_facet | Mariana Mescouto Lopes Thaís Ribeiro Brito Josiane Fonseca Lage Thaís Correia Costa Marta Maria dos Santos Fontes Nick Vergara Lopes Serão Tiago Antônio de Oliveira Mendes Ricardo Andrade Reis Renata Veroneze Fabyano Fonseca e Silva Marcio de Souza Duarte |
| author_sort | Mariana Mescouto Lopes |
| collection | DOAJ |
| description | Vitamin B and trace minerals are crucial molecular signals involved in many biological pathways; however, their bioavailability is compromised in high-producing ruminant animals. So far, studies have mainly focused on the effects of these micronutrients on animal performance, but their use in a rumen-protected form and their impact on liver metabolism in finishing beef cattle is poorly known. We used a shotgun proteomic approach combined with biological network analyses to assess the effects of a rumen-protected B-vitamin blend, as well as those of hydroxy trace minerals, on the hepatic proteome. A total of 20 non-castrated Nellore males with 353 ± 43 kg of initial body weight were randomly assigned to one of the following treatments: CTRL—inorganic trace minerals without supplementation of a protected vitamin B blend, or SUP—supplementation of hydroxy trace minerals and a protected vitamin B blend. All animals were fed the same amount of the experimental diet for 106 days, and liver biopsies were performed at the end of the experimental period. Supplemented animals showed 37 up-regulated proteins (<i>p</i> < 0.10), and the enrichment analysis revealed that these proteins were involved in protein folding (<i>p</i> = 0.04), mitochondrial respiratory chain complex I (<i>p</i> = 0.01) and IV (<i>p</i> = 0.01), chaperonin-containing T-complex 2 (<i>p</i> = 0.01), glutathione metabolism (<i>p</i> < 0.01), and other aspects linked to oxidative-stress responses. These results indicate that rumen-protected vitamin B and hydroxy trace mineral supplementation during the finishing phase alters the abundance of proteins associated with the electron transport chain and other oxidation–reduction pathways, boosting the production of reactive oxygen species, which appear to modulate proteins linked to oxidative-damage responses to maintain cellular homeostasis. |
| first_indexed | 2024-03-10T09:59:01Z |
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| id | doaj.art-c1dedf9069be4a8cac0ea3bd3484615c |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T09:59:01Z |
| publishDate | 2021-06-01 |
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| series | Animals |
| spelling | doaj.art-c1dedf9069be4a8cac0ea3bd3484615c2023-11-22T02:07:30ZengMDPI AGAnimals2076-26152021-06-01117193410.3390/ani11071934Proteomic Analysis of Liver from Finishing Beef Cattle Supplemented with a Rumen-Protected B-Vitamin Blend and Hydroxy Trace MineralsMariana Mescouto Lopes0Thaís Ribeiro Brito1Josiane Fonseca Lage2Thaís Correia Costa3Marta Maria dos Santos Fontes4Nick Vergara Lopes Serão5Tiago Antônio de Oliveira Mendes6Ricardo Andrade Reis7Renata Veroneze8Fabyano Fonseca e Silva9Marcio de Souza Duarte10Department of Animal Science, Universidade Federal de Viçosa, Viçosa 36570-000, BrazilDepartment of Animal Science, São Paulo State University, Jaboticabal 14887-900, BrazilTrouw Nutrition, Research and Development, Campinas 13080-650, BrazilDepartment of Animal Science, Universidade Federal de Viçosa, Viçosa 36570-000, BrazilDepartment of Animal Science, Universidade Federal de Viçosa, Viçosa 36570-000, BrazilDepartment of Animal Science, Iowa State University, Ames, IA 50011, USADepartment of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa 36570-000, BrazilDepartment of Animal Science, São Paulo State University, Jaboticabal 14887-900, BrazilDepartment of Animal Science, Universidade Federal de Viçosa, Viçosa 36570-000, BrazilDepartment of Animal Science, Universidade Federal de Viçosa, Viçosa 36570-000, BrazilDepartment of Animal Science, Universidade Federal de Viçosa, Viçosa 36570-000, BrazilVitamin B and trace minerals are crucial molecular signals involved in many biological pathways; however, their bioavailability is compromised in high-producing ruminant animals. So far, studies have mainly focused on the effects of these micronutrients on animal performance, but their use in a rumen-protected form and their impact on liver metabolism in finishing beef cattle is poorly known. We used a shotgun proteomic approach combined with biological network analyses to assess the effects of a rumen-protected B-vitamin blend, as well as those of hydroxy trace minerals, on the hepatic proteome. A total of 20 non-castrated Nellore males with 353 ± 43 kg of initial body weight were randomly assigned to one of the following treatments: CTRL—inorganic trace minerals without supplementation of a protected vitamin B blend, or SUP—supplementation of hydroxy trace minerals and a protected vitamin B blend. All animals were fed the same amount of the experimental diet for 106 days, and liver biopsies were performed at the end of the experimental period. Supplemented animals showed 37 up-regulated proteins (<i>p</i> < 0.10), and the enrichment analysis revealed that these proteins were involved in protein folding (<i>p</i> = 0.04), mitochondrial respiratory chain complex I (<i>p</i> = 0.01) and IV (<i>p</i> = 0.01), chaperonin-containing T-complex 2 (<i>p</i> = 0.01), glutathione metabolism (<i>p</i> < 0.01), and other aspects linked to oxidative-stress responses. These results indicate that rumen-protected vitamin B and hydroxy trace mineral supplementation during the finishing phase alters the abundance of proteins associated with the electron transport chain and other oxidation–reduction pathways, boosting the production of reactive oxygen species, which appear to modulate proteins linked to oxidative-damage responses to maintain cellular homeostasis.https://www.mdpi.com/2076-2615/11/7/1934beef cattleproteomicsvitamin Bhydroxyl trace mineralsliver metabolism |
| spellingShingle | Mariana Mescouto Lopes Thaís Ribeiro Brito Josiane Fonseca Lage Thaís Correia Costa Marta Maria dos Santos Fontes Nick Vergara Lopes Serão Tiago Antônio de Oliveira Mendes Ricardo Andrade Reis Renata Veroneze Fabyano Fonseca e Silva Marcio de Souza Duarte Proteomic Analysis of Liver from Finishing Beef Cattle Supplemented with a Rumen-Protected B-Vitamin Blend and Hydroxy Trace Minerals Animals beef cattle proteomics vitamin B hydroxyl trace minerals liver metabolism |
| title | Proteomic Analysis of Liver from Finishing Beef Cattle Supplemented with a Rumen-Protected B-Vitamin Blend and Hydroxy Trace Minerals |
| title_full | Proteomic Analysis of Liver from Finishing Beef Cattle Supplemented with a Rumen-Protected B-Vitamin Blend and Hydroxy Trace Minerals |
| title_fullStr | Proteomic Analysis of Liver from Finishing Beef Cattle Supplemented with a Rumen-Protected B-Vitamin Blend and Hydroxy Trace Minerals |
| title_full_unstemmed | Proteomic Analysis of Liver from Finishing Beef Cattle Supplemented with a Rumen-Protected B-Vitamin Blend and Hydroxy Trace Minerals |
| title_short | Proteomic Analysis of Liver from Finishing Beef Cattle Supplemented with a Rumen-Protected B-Vitamin Blend and Hydroxy Trace Minerals |
| title_sort | proteomic analysis of liver from finishing beef cattle supplemented with a rumen protected b vitamin blend and hydroxy trace minerals |
| topic | beef cattle proteomics vitamin B hydroxyl trace minerals liver metabolism |
| url | https://www.mdpi.com/2076-2615/11/7/1934 |
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