Virome of wild rats (Rattus norvegicus) captured far from pig farms in Jiangsu province of China reveals novel porcine circovirus type 2d (PCV2d) sequences
Abstract Background Porcine circovirus type 2 (PCV2) has caused great economic losses in the global pig industry. There have been published records of wild rats acting as the reservoirs of PCV2 (only PCV2a and PCV2b), but almost all of which were related to the PCV2-infected swine herds. Results In...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-03-01
|
| Series: | Virology Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12985-023-02005-2 |
| _version_ | 1797865630156718080 |
|---|---|
| author | Min Zhao Siwen Bao Diandian Xu Jingxian He Han Zhang Likai Ji Shixing Yang Xiaochun Wang Quan Shen Jia Liu Qing Zhang Xiao Ma Wen Zhang Tongling Shan |
| author_facet | Min Zhao Siwen Bao Diandian Xu Jingxian He Han Zhang Likai Ji Shixing Yang Xiaochun Wang Quan Shen Jia Liu Qing Zhang Xiao Ma Wen Zhang Tongling Shan |
| author_sort | Min Zhao |
| collection | DOAJ |
| description | Abstract Background Porcine circovirus type 2 (PCV2) has caused great economic losses in the global pig industry. There have been published records of wild rats acting as the reservoirs of PCV2 (only PCV2a and PCV2b), but almost all of which were related to the PCV2-infected swine herds. Results In this study, we carried out the detection, amplification, and characterization of novel PCV2 strains in wild rats that were captured far from pig farms. Nested PCR assay demonstrated that the kidney, heart, lung, liver, pancreas, and large and small intestines of rats were screened positive for PCV2. We subsequently sequenced two full genomes of PCV2 in positive sample pools, designated as js2021-Rt001 and js2021-Rt002. Genome sequence analysis indicated that they had the highest similarity to nucleotide sequences of porcine-origin PCV2 isolates in Vietnam. Phylogenetically, js2021-Rt001 and js2021-Rt002 were a part of the PCV2d genotype cluster, which is a predominant genotype circulating worldwide in recent years. The antibody recognition regions, immunodominant decoy epitope, and heparin sulfate binding motif of the two complete genome sequences coincided with those previously reported. Conclusions Our research reported the genomic characterization of two novel PCV2 strains (js2021-Rt001 and js2021-Rt002) and provided the first supported evidence that PCV2d could naturally infect wild rats in China. However, whether the newly identified strains have potential for circulating in nature in vertical and horizontal transmission or inter-species jumping between rats and pigs needs further research. |
| first_indexed | 2024-04-09T23:11:12Z |
| format | Article |
| id | doaj.art-c1e1a41d191440d0a4ec53ad1541bcf5 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-04-09T23:11:12Z |
| publishDate | 2023-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-c1e1a41d191440d0a4ec53ad1541bcf52023-03-22T10:24:53ZengBMCVirology Journal1743-422X2023-03-0120111010.1186/s12985-023-02005-2Virome of wild rats (Rattus norvegicus) captured far from pig farms in Jiangsu province of China reveals novel porcine circovirus type 2d (PCV2d) sequencesMin Zhao0Siwen Bao1Diandian Xu2Jingxian He3Han Zhang4Likai Ji5Shixing Yang6Xiaochun Wang7Quan Shen8Jia Liu9Qing Zhang10Xiao Ma11Wen Zhang12Tongling Shan13Department of Microbiology, School of Medicine, Jiangsu UniversityDepartment of Microbiology, School of Medicine, Jiangsu UniversityDepartment of Microbiology, School of Medicine, Jiangsu UniversitySchool of Medicine, Suzhou UniversityDepartment of Microbiology, School of Medicine, Jiangsu UniversityDepartment of Microbiology, School of Medicine, Jiangsu UniversityDepartment of Microbiology, School of Medicine, Jiangsu UniversityDepartment of Microbiology, School of Medicine, Jiangsu UniversityDepartment of Microbiology, School of Medicine, Jiangsu UniversityQinghai Institute of Endemic Disease Prevention and ControlQinghai Institute of Endemic Disease Prevention and ControlQinghai Institute of Endemic Disease Prevention and ControlDepartment of Microbiology, School of Medicine, Jiangsu UniversityShanghai Veterinary Research Institute, Chinese Academy of Agricultural SciencesAbstract Background Porcine circovirus type 2 (PCV2) has caused great economic losses in the global pig industry. There have been published records of wild rats acting as the reservoirs of PCV2 (only PCV2a and PCV2b), but almost all of which were related to the PCV2-infected swine herds. Results In this study, we carried out the detection, amplification, and characterization of novel PCV2 strains in wild rats that were captured far from pig farms. Nested PCR assay demonstrated that the kidney, heart, lung, liver, pancreas, and large and small intestines of rats were screened positive for PCV2. We subsequently sequenced two full genomes of PCV2 in positive sample pools, designated as js2021-Rt001 and js2021-Rt002. Genome sequence analysis indicated that they had the highest similarity to nucleotide sequences of porcine-origin PCV2 isolates in Vietnam. Phylogenetically, js2021-Rt001 and js2021-Rt002 were a part of the PCV2d genotype cluster, which is a predominant genotype circulating worldwide in recent years. The antibody recognition regions, immunodominant decoy epitope, and heparin sulfate binding motif of the two complete genome sequences coincided with those previously reported. Conclusions Our research reported the genomic characterization of two novel PCV2 strains (js2021-Rt001 and js2021-Rt002) and provided the first supported evidence that PCV2d could naturally infect wild rats in China. However, whether the newly identified strains have potential for circulating in nature in vertical and horizontal transmission or inter-species jumping between rats and pigs needs further research.https://doi.org/10.1186/s12985-023-02005-2PCV2Wild ratPhylogenetic analysis |
| spellingShingle | Min Zhao Siwen Bao Diandian Xu Jingxian He Han Zhang Likai Ji Shixing Yang Xiaochun Wang Quan Shen Jia Liu Qing Zhang Xiao Ma Wen Zhang Tongling Shan Virome of wild rats (Rattus norvegicus) captured far from pig farms in Jiangsu province of China reveals novel porcine circovirus type 2d (PCV2d) sequences Virology Journal PCV2 Wild rat Phylogenetic analysis |
| title | Virome of wild rats (Rattus norvegicus) captured far from pig farms in Jiangsu province of China reveals novel porcine circovirus type 2d (PCV2d) sequences |
| title_full | Virome of wild rats (Rattus norvegicus) captured far from pig farms in Jiangsu province of China reveals novel porcine circovirus type 2d (PCV2d) sequences |
| title_fullStr | Virome of wild rats (Rattus norvegicus) captured far from pig farms in Jiangsu province of China reveals novel porcine circovirus type 2d (PCV2d) sequences |
| title_full_unstemmed | Virome of wild rats (Rattus norvegicus) captured far from pig farms in Jiangsu province of China reveals novel porcine circovirus type 2d (PCV2d) sequences |
| title_short | Virome of wild rats (Rattus norvegicus) captured far from pig farms in Jiangsu province of China reveals novel porcine circovirus type 2d (PCV2d) sequences |
| title_sort | virome of wild rats rattus norvegicus captured far from pig farms in jiangsu province of china reveals novel porcine circovirus type 2d pcv2d sequences |
| topic | PCV2 Wild rat Phylogenetic analysis |
| url | https://doi.org/10.1186/s12985-023-02005-2 |
| work_keys_str_mv | AT minzhao viromeofwildratsrattusnorvegicuscapturedfarfrompigfarmsinjiangsuprovinceofchinarevealsnovelporcinecircovirustype2dpcv2dsequences AT siwenbao viromeofwildratsrattusnorvegicuscapturedfarfrompigfarmsinjiangsuprovinceofchinarevealsnovelporcinecircovirustype2dpcv2dsequences AT diandianxu viromeofwildratsrattusnorvegicuscapturedfarfrompigfarmsinjiangsuprovinceofchinarevealsnovelporcinecircovirustype2dpcv2dsequences AT jingxianhe viromeofwildratsrattusnorvegicuscapturedfarfrompigfarmsinjiangsuprovinceofchinarevealsnovelporcinecircovirustype2dpcv2dsequences AT hanzhang viromeofwildratsrattusnorvegicuscapturedfarfrompigfarmsinjiangsuprovinceofchinarevealsnovelporcinecircovirustype2dpcv2dsequences AT likaiji viromeofwildratsrattusnorvegicuscapturedfarfrompigfarmsinjiangsuprovinceofchinarevealsnovelporcinecircovirustype2dpcv2dsequences AT shixingyang viromeofwildratsrattusnorvegicuscapturedfarfrompigfarmsinjiangsuprovinceofchinarevealsnovelporcinecircovirustype2dpcv2dsequences AT xiaochunwang viromeofwildratsrattusnorvegicuscapturedfarfrompigfarmsinjiangsuprovinceofchinarevealsnovelporcinecircovirustype2dpcv2dsequences AT quanshen viromeofwildratsrattusnorvegicuscapturedfarfrompigfarmsinjiangsuprovinceofchinarevealsnovelporcinecircovirustype2dpcv2dsequences AT jialiu viromeofwildratsrattusnorvegicuscapturedfarfrompigfarmsinjiangsuprovinceofchinarevealsnovelporcinecircovirustype2dpcv2dsequences AT qingzhang viromeofwildratsrattusnorvegicuscapturedfarfrompigfarmsinjiangsuprovinceofchinarevealsnovelporcinecircovirustype2dpcv2dsequences AT xiaoma viromeofwildratsrattusnorvegicuscapturedfarfrompigfarmsinjiangsuprovinceofchinarevealsnovelporcinecircovirustype2dpcv2dsequences AT wenzhang viromeofwildratsrattusnorvegicuscapturedfarfrompigfarmsinjiangsuprovinceofchinarevealsnovelporcinecircovirustype2dpcv2dsequences AT tonglingshan viromeofwildratsrattusnorvegicuscapturedfarfrompigfarmsinjiangsuprovinceofchinarevealsnovelporcinecircovirustype2dpcv2dsequences |